Bral1: Its role in diffusion barrier formation and conduction velocity in the CNS

Yoko Bekku, Lýdia Vargová, Yoshinobu Goto, Ivan Vorísek, Lesia Dmytrenko, Masahiro Narasaki, Aiji Ohtsuka, Reinhard Fässler, Yoshifumi Ninomiya, Eva Syková, Toshitaka Oohashi

Research output: Contribution to journalArticle

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Abstract

At the nodes of Ranvier, excitable axon membranes are exposed directly to the extracellular fluid. Cations are accumulated and depleted in the local extracellular nodal region during action potential propagation, but the impact of the extranodal micromilieu on signal propagation still remains unclear. Brain-specific hyaluronan-binding link protein, Bral1, colocalizes and forms complexes with negatively charged extracellular matrix (ECM) proteins, such as versican V2 and brevican, at the nodes of Ranvier in the myelinated white matter. The link protein family, including Bral1, appears to be the linchpin of these hyaluronan-bound ECM complexes. Here we report that the hyaluronan-associated ECM no longer shows a nodal pattern and that CNS nerve conduction is markedly decreased in Bral1-deficient mice even though there were no differences between wild-type and mutant mice in the clustering or transition of ion channels at the nodes or in the tissue morphology around the nodes of Ranvier. However, changes in the extracellular space diffusion parameters, measured by the real-time iontophoretic method and diffusion-weighted magnetic resonance imaging (MRI), suggest a reduction in the diffusion hindrances in the white matter of mutant mice. These findings provide a better understanding of the mechanisms underlying the accumulation of cations due to diffusion barriers around the nodes during saltatory conduction, which further implies the importance of the Bral1-based extramilieu for neuronal conductivity.

Original languageEnglish
Pages (from-to)3113-3123
Number of pages11
JournalJournal of Neuroscience
Volume30
Issue number8
DOIs
Publication statusPublished - Feb 24 2010

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Ranvier's Nodes
Hyaluronic Acid
Extracellular Matrix
Brevican
Cations
Versicans
CD44 Antigens
Diffusion Magnetic Resonance Imaging
Extracellular Matrix Proteins
Neural Conduction
Extracellular Fluid
Extracellular Space
Ion Channels
Action Potentials
Cluster Analysis
Axons
Membranes
Brain
White Matter
link protein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bral1 : Its role in diffusion barrier formation and conduction velocity in the CNS. / Bekku, Yoko; Vargová, Lýdia; Goto, Yoshinobu; Vorísek, Ivan; Dmytrenko, Lesia; Narasaki, Masahiro; Ohtsuka, Aiji; Fässler, Reinhard; Ninomiya, Yoshifumi; Syková, Eva; Oohashi, Toshitaka.

In: Journal of Neuroscience, Vol. 30, No. 8, 24.02.2010, p. 3113-3123.

Research output: Contribution to journalArticle

Bekku, Y, Vargová, L, Goto, Y, Vorísek, I, Dmytrenko, L, Narasaki, M, Ohtsuka, A, Fässler, R, Ninomiya, Y, Syková, E & Oohashi, T 2010, 'Bral1: Its role in diffusion barrier formation and conduction velocity in the CNS', Journal of Neuroscience, vol. 30, no. 8, pp. 3113-3123. https://doi.org/10.1523/JNEUROSCI.5598-09.2010
Bekku Y, Vargová L, Goto Y, Vorísek I, Dmytrenko L, Narasaki M et al. Bral1: Its role in diffusion barrier formation and conduction velocity in the CNS. Journal of Neuroscience. 2010 Feb 24;30(8):3113-3123. https://doi.org/10.1523/JNEUROSCI.5598-09.2010
Bekku, Yoko ; Vargová, Lýdia ; Goto, Yoshinobu ; Vorísek, Ivan ; Dmytrenko, Lesia ; Narasaki, Masahiro ; Ohtsuka, Aiji ; Fässler, Reinhard ; Ninomiya, Yoshifumi ; Syková, Eva ; Oohashi, Toshitaka. / Bral1 : Its role in diffusion barrier formation and conduction velocity in the CNS. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 8. pp. 3113-3123.
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