Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems

Toshitaka Oohashi, Satoshi Hirakawa, Yoko Bekku, Uwe Rauch, Dieter R. Zimmermann, Wei Dong Su, Aiji Ohtsuka, Takuro Murakami, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

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Abstract

Bral1, a brain-specific hyaluronan-binding protein, has been cloned recently. To gain insight into the role of Bral1, we generated a specific antibody against this protein. We have examined the detailed localization pattern of Bral1 protein and compared it with that of other members of the lectican proteoglycan family, such as brevican and versican, with which Bral1 is predicted to interact. The immunoreactivity of Bral1 antibody was predominantly observed in myelinated fiber tracts in the adult brain and could be detected at P20 in the white matter of the developing cerebellum, suggesting that expression starts when axonal myelination takes place. Furthermore, immunostaining demonstrated that Bral1 colocalized with the versican V2 isoform at the nodes of Ranvier. The present data suggest that Bral1 may play a pivotal role in the formation of the hyaluronan-associated matrix in the CNS that facilitates neuronal conduction by forming an ion diffusion barrier at the nodes.

Original languageEnglish
Pages (from-to)43-57
Number of pages15
JournalMolecular and Cellular Neuroscience
Volume19
Issue number1
DOIs
Publication statusPublished - 2002

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Versicans
Ranvier's Nodes
Brevican
Protein Isoforms
Central Nervous System
CD44 Antigens
Antibodies
Brain
Proteoglycans
Hyaluronic Acid
Cerebellum
Proteins
Ions
link protein

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems. / Oohashi, Toshitaka; Hirakawa, Satoshi; Bekku, Yoko; Rauch, Uwe; Zimmermann, Dieter R.; Su, Wei Dong; Ohtsuka, Aiji; Murakami, Takuro; Ninomiya, Yoshifumi.

In: Molecular and Cellular Neuroscience, Vol. 19, No. 1, 2002, p. 43-57.

Research output: Contribution to journalArticle

Oohashi, Toshitaka ; Hirakawa, Satoshi ; Bekku, Yoko ; Rauch, Uwe ; Zimmermann, Dieter R. ; Su, Wei Dong ; Ohtsuka, Aiji ; Murakami, Takuro ; Ninomiya, Yoshifumi. / Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems. In: Molecular and Cellular Neuroscience. 2002 ; Vol. 19, No. 1. pp. 43-57.
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AU - Hirakawa, Satoshi

AU - Bekku, Yoko

AU - Rauch, Uwe

AU - Zimmermann, Dieter R.

AU - Su, Wei Dong

AU - Ohtsuka, Aiji

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