Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei

H. Ichikawa, T. Yabuuchi, H. W. Jin, R. Terayama, T. Yamaai, T. Deguchi, Hiroshi Kamioka, T. Takano-Yamamoto, T. Sugimoto

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat trigeminal ganglion (TG). The immunoreactivity (IR) was detected in 46% of TG neurons. These neurons were mostly small- or medium-sized (range, 149.7-1246.3 μm2; mean ± SD = 373.4 ± 151.6 μm2). A double immunofluorescence method also revealed that 54% of BDNF-immunoreactive (IR) neurons were immunoreactive for calcitonin-gene-related peptide. In addition, 93% of BDNF-IR TG neurons contained vanilloid receptor subtype 1. However, the co-expression of BDNF and vanilloid receptor 1-like receptor was very rare (less than 1%). In the trigeminal sensory nuclei, laminae II of the medullary dorsal horn was abundant in presumed BDNF-IR axon terminals. Such profiles were also detected in the dorsolateral part of the subnucleus oralis. The retrograde tracing and immunohistochemical methods demonstrated that BDNF-IR was common among cutaneous TG neurons (47%) but not tooth pulp TG neurons (13%). The present study indicates that BDNF-IR TG neurons have unmyelinated axons and project to the superficial medullary dorsal horn. It is likely that BDNF-containing neurons in both the trigeminal and spinal sensory systems have similarities in morphology and function. However, the content of BDNF in TG neurons probably depends on their peripheral targets. BDNF seems to convey nociceptive cutaneous input to the trigeminal sensory nuclei.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalBrain Research
Volume1081
Issue number1
DOIs
Publication statusPublished - Apr 7 2006

Fingerprint

Trigeminal Nuclei
Trigeminal Ganglion
Brain-Derived Neurotrophic Factor
Sensory Receptor Cells
Neurons
Substantia Gelatinosa
trkB Receptor
TRPV Cation Channels
Skin
Calcitonin Gene-Related Peptide
Presynaptic Terminals
Fluorescent Antibody Technique
Axons
Tooth
Immunohistochemistry

Keywords

  • Brain-derived neurotrophic factor
  • Calcitonin-gene-related peptide
  • Skin
  • Tooth pulp
  • Trigeminal ganglion
  • VR1
  • VRL-1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei. / Ichikawa, H.; Yabuuchi, T.; Jin, H. W.; Terayama, R.; Yamaai, T.; Deguchi, T.; Kamioka, Hiroshi; Takano-Yamamoto, T.; Sugimoto, T.

In: Brain Research, Vol. 1081, No. 1, 07.04.2006, p. 113-118.

Research output: Contribution to journalArticle

Ichikawa, H, Yabuuchi, T, Jin, HW, Terayama, R, Yamaai, T, Deguchi, T, Kamioka, H, Takano-Yamamoto, T & Sugimoto, T 2006, 'Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei', Brain Research, vol. 1081, no. 1, pp. 113-118. https://doi.org/10.1016/j.brainres.2006.01.027
Ichikawa, H. ; Yabuuchi, T. ; Jin, H. W. ; Terayama, R. ; Yamaai, T. ; Deguchi, T. ; Kamioka, Hiroshi ; Takano-Yamamoto, T. ; Sugimoto, T. / Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei. In: Brain Research. 2006 ; Vol. 1081, No. 1. pp. 113-118.
@article{4f86b33ff0914b6191b3af3f11dd9eff,
title = "Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei",
abstract = "Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat trigeminal ganglion (TG). The immunoreactivity (IR) was detected in 46{\%} of TG neurons. These neurons were mostly small- or medium-sized (range, 149.7-1246.3 μm2; mean ± SD = 373.4 ± 151.6 μm2). A double immunofluorescence method also revealed that 54{\%} of BDNF-immunoreactive (IR) neurons were immunoreactive for calcitonin-gene-related peptide. In addition, 93{\%} of BDNF-IR TG neurons contained vanilloid receptor subtype 1. However, the co-expression of BDNF and vanilloid receptor 1-like receptor was very rare (less than 1{\%}). In the trigeminal sensory nuclei, laminae II of the medullary dorsal horn was abundant in presumed BDNF-IR axon terminals. Such profiles were also detected in the dorsolateral part of the subnucleus oralis. The retrograde tracing and immunohistochemical methods demonstrated that BDNF-IR was common among cutaneous TG neurons (47{\%}) but not tooth pulp TG neurons (13{\%}). The present study indicates that BDNF-IR TG neurons have unmyelinated axons and project to the superficial medullary dorsal horn. It is likely that BDNF-containing neurons in both the trigeminal and spinal sensory systems have similarities in morphology and function. However, the content of BDNF in TG neurons probably depends on their peripheral targets. BDNF seems to convey nociceptive cutaneous input to the trigeminal sensory nuclei.",
keywords = "Brain-derived neurotrophic factor, Calcitonin-gene-related peptide, Skin, Tooth pulp, Trigeminal ganglion, VR1, VRL-1",
author = "H. Ichikawa and T. Yabuuchi and Jin, {H. W.} and R. Terayama and T. Yamaai and T. Deguchi and Hiroshi Kamioka and T. Takano-Yamamoto and T. Sugimoto",
year = "2006",
month = "4",
day = "7",
doi = "10.1016/j.brainres.2006.01.027",
language = "English",
volume = "1081",
pages = "113--118",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei

AU - Ichikawa, H.

AU - Yabuuchi, T.

AU - Jin, H. W.

AU - Terayama, R.

AU - Yamaai, T.

AU - Deguchi, T.

AU - Kamioka, Hiroshi

AU - Takano-Yamamoto, T.

AU - Sugimoto, T.

PY - 2006/4/7

Y1 - 2006/4/7

N2 - Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat trigeminal ganglion (TG). The immunoreactivity (IR) was detected in 46% of TG neurons. These neurons were mostly small- or medium-sized (range, 149.7-1246.3 μm2; mean ± SD = 373.4 ± 151.6 μm2). A double immunofluorescence method also revealed that 54% of BDNF-immunoreactive (IR) neurons were immunoreactive for calcitonin-gene-related peptide. In addition, 93% of BDNF-IR TG neurons contained vanilloid receptor subtype 1. However, the co-expression of BDNF and vanilloid receptor 1-like receptor was very rare (less than 1%). In the trigeminal sensory nuclei, laminae II of the medullary dorsal horn was abundant in presumed BDNF-IR axon terminals. Such profiles were also detected in the dorsolateral part of the subnucleus oralis. The retrograde tracing and immunohistochemical methods demonstrated that BDNF-IR was common among cutaneous TG neurons (47%) but not tooth pulp TG neurons (13%). The present study indicates that BDNF-IR TG neurons have unmyelinated axons and project to the superficial medullary dorsal horn. It is likely that BDNF-containing neurons in both the trigeminal and spinal sensory systems have similarities in morphology and function. However, the content of BDNF in TG neurons probably depends on their peripheral targets. BDNF seems to convey nociceptive cutaneous input to the trigeminal sensory nuclei.

AB - Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat trigeminal ganglion (TG). The immunoreactivity (IR) was detected in 46% of TG neurons. These neurons were mostly small- or medium-sized (range, 149.7-1246.3 μm2; mean ± SD = 373.4 ± 151.6 μm2). A double immunofluorescence method also revealed that 54% of BDNF-immunoreactive (IR) neurons were immunoreactive for calcitonin-gene-related peptide. In addition, 93% of BDNF-IR TG neurons contained vanilloid receptor subtype 1. However, the co-expression of BDNF and vanilloid receptor 1-like receptor was very rare (less than 1%). In the trigeminal sensory nuclei, laminae II of the medullary dorsal horn was abundant in presumed BDNF-IR axon terminals. Such profiles were also detected in the dorsolateral part of the subnucleus oralis. The retrograde tracing and immunohistochemical methods demonstrated that BDNF-IR was common among cutaneous TG neurons (47%) but not tooth pulp TG neurons (13%). The present study indicates that BDNF-IR TG neurons have unmyelinated axons and project to the superficial medullary dorsal horn. It is likely that BDNF-containing neurons in both the trigeminal and spinal sensory systems have similarities in morphology and function. However, the content of BDNF in TG neurons probably depends on their peripheral targets. BDNF seems to convey nociceptive cutaneous input to the trigeminal sensory nuclei.

KW - Brain-derived neurotrophic factor

KW - Calcitonin-gene-related peptide

KW - Skin

KW - Tooth pulp

KW - Trigeminal ganglion

KW - VR1

KW - VRL-1

UR - http://www.scopus.com/inward/record.url?scp=33645734353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645734353&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2006.01.027

DO - 10.1016/j.brainres.2006.01.027

M3 - Article

C2 - 16510129

AN - SCOPUS:33645734353

VL - 1081

SP - 113

EP - 118

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -