Brain-derived neurotrophic factor-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia; co-expression with other neurochemical substances

H. Ichikawa, R. Terayama, T. Yamaai, Z. Yan, T. Sugimoto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat vagal and glossopharyngeal sensory ganglia. In the jugular, petrosal and nodose ganglia, 56.1 ± 5.5%, 52.4 ± 9.4% and 80.0 ± 3.0% of sensory neurons, respectively, were immunoreactive for BDNF. These neurons were small- to medium-sized and observed throughout the ganglia. In the solitary tract nucleus, the neuropil showed BDNF immunoreactivity. A double immunofluorescence method demonstrated that BDNF-immunoreactive neurons were also immunoreactive for calcitonin gene-related peptide (CGRP), P2X3 receptor, the capsaicin receptor (VR1) or vanilloid receptor 1-like receptor (VRL-1) in the jugular (CGRP, 43.5%; P2X3 receptor, 51.1%; VR1, 71.7%; VRL-1, 0.5%), petrosal (CGRP, 33.2%; P2X3 receptor, 58.4%; VR1, 54.2%; VRL-1, 23.3%) and nodose ganglia (CGRP, 1.8%; P2X3 receptor, 49.1%; VR1, 70.7%; VRL-1, 11.5%). The co-expression with tyrosine hydroxylase was also detected in the petrosal (2.9%) and nodose ganglia (2.2%). However, BDNF-immunoreactive neurons were devoid of parvalbumin in these ganglia. The present findings suggest that BDNF-containing vagal and glossopharyngeal sensory neurons have nociceptive and chemoreceptive functions.

Original languageEnglish
Pages (from-to)93-99
Number of pages7
JournalBrain Research
Volume1155
Issue number1
DOIs
Publication statusPublished - Jun 25 2007

Fingerprint

Sensory Ganglia
Brain-Derived Neurotrophic Factor
Purinergic P2X3 Receptors
Nodose Ganglion
Neurons
Calcitonin Gene-Related Peptide
Sensory Receptor Cells
Ganglia
Neck
Calcitonin Gene-Related Peptide Receptors
TRPV Cation Channels
Parvalbumins
Solitary Nucleus
Neuropil
Tyrosine 3-Monooxygenase
Fluorescent Antibody Technique
Immunohistochemistry

Keywords

  • Brain-derived neurotrophic factor
  • Immunohistochemistry
  • Jugular ganglion
  • Nodose ganglion
  • Petrosal ganglion
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Brain-derived neurotrophic factor-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia; co-expression with other neurochemical substances. / Ichikawa, H.; Terayama, R.; Yamaai, T.; Yan, Z.; Sugimoto, T.

In: Brain Research, Vol. 1155, No. 1, 25.06.2007, p. 93-99.

Research output: Contribution to journalArticle

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abstract = "Immunohistochemistry for brain-derived neurotrophic factor (BDNF) was performed on the rat vagal and glossopharyngeal sensory ganglia. In the jugular, petrosal and nodose ganglia, 56.1 ± 5.5{\%}, 52.4 ± 9.4{\%} and 80.0 ± 3.0{\%} of sensory neurons, respectively, were immunoreactive for BDNF. These neurons were small- to medium-sized and observed throughout the ganglia. In the solitary tract nucleus, the neuropil showed BDNF immunoreactivity. A double immunofluorescence method demonstrated that BDNF-immunoreactive neurons were also immunoreactive for calcitonin gene-related peptide (CGRP), P2X3 receptor, the capsaicin receptor (VR1) or vanilloid receptor 1-like receptor (VRL-1) in the jugular (CGRP, 43.5{\%}; P2X3 receptor, 51.1{\%}; VR1, 71.7{\%}; VRL-1, 0.5{\%}), petrosal (CGRP, 33.2{\%}; P2X3 receptor, 58.4{\%}; VR1, 54.2{\%}; VRL-1, 23.3{\%}) and nodose ganglia (CGRP, 1.8{\%}; P2X3 receptor, 49.1{\%}; VR1, 70.7{\%}; VRL-1, 11.5{\%}). The co-expression with tyrosine hydroxylase was also detected in the petrosal (2.9{\%}) and nodose ganglia (2.2{\%}). However, BDNF-immunoreactive neurons were devoid of parvalbumin in these ganglia. The present findings suggest that BDNF-containing vagal and glossopharyngeal sensory neurons have nociceptive and chemoreceptive functions.",
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