TY - JOUR
T1 - Bovine trabecular cells produce TIMP-1 and MMP-2 in response to mechanical stretching
AU - Okada, Yumiko
AU - Matsuo, Toshihiko
AU - Ohtsuki, Hiroshi
N1 - Funding Information:
The authors are grateful to Dr Nobuhiko Matsuo, Professor Emeritus in the Department of Ophthalmology, Okayama University Medical School, for his support, and Dr Shoichi Sawaguchi in the Department of Ophthalmology, Niigata University School of Medicine, for his advice on zymography. They also thank Yukinari Isomoto at the Central Research Laboratories at Okayama University Medical School for his help in densitometric analysis of zymography. This work is supported in part by a Grant-in-Aid (B2-09470380) from the Ministry of Education, Science, Sports, and Culture of the Japanese Government.
PY - 1998/3
Y1 - 1998/3
N2 - Bovine trabecular cells in growth phase were exposed to cyclic mechanical stretching of the bottom of a culture dish at a cycle of 30 seconds for 72 hours. The stretched cells produced significantly larger amounts of metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1) after 72 hours, compared with cells in nonstretched control. In contrast, TIMP-2 and MMP-9 levels were not influenced by mechanical stretching. Trabecular cells would modify extracellular matrix in response to such mechanical stimuli as bending of trabecular meshwork or aqueous flow by the production of TIMP-1 and MMP-2.
AB - Bovine trabecular cells in growth phase were exposed to cyclic mechanical stretching of the bottom of a culture dish at a cycle of 30 seconds for 72 hours. The stretched cells produced significantly larger amounts of metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1) after 72 hours, compared with cells in nonstretched control. In contrast, TIMP-2 and MMP-9 levels were not influenced by mechanical stretching. Trabecular cells would modify extracellular matrix in response to such mechanical stimuli as bending of trabecular meshwork or aqueous flow by the production of TIMP-1 and MMP-2.
KW - Cyclic mechanical stretching
KW - Matrix metalloproteinase-2 and 9 (MMP-2 and MMP-9)
KW - Tissue inhibitor of metalloproteinase-1 and 2 (TIMP-1 and TIMP-2)
KW - Trabecular cells
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U2 - 10.1016/S0021-5155(97)00129-9
DO - 10.1016/S0021-5155(97)00129-9
M3 - Article
C2 - 9587839
AN - SCOPUS:0032029157
SN - 0021-5155
VL - 42
SP - 90
EP - 94
JO - Japanese Journal of Ophthalmology
JF - Japanese Journal of Ophthalmology
IS - 2
ER -