Both focal adhesion kinase and c-Ras are required for the enhanced matrix metalloproteinase 9 secretion by fibronectin in ovarian cancer cells

Kiyosumi Shibata, Fumitaka Kikkawa, Akihiro Nawa, Aye Aye Thant, Keiji Naruse, Shigehiko Mizutani, Michinari Hamaguchi

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Cell adhesion to the extracellular matrix appears to trigger a cascade of intracellular signalings. We have shown previously that treatment of ovarian cancer cells with peritoneal conditioned medium or purified fibronectin (FN) activated matrix metalloproteinase 9 secretion and, thereby, cancer cell invasion. By use of antisense oligonucleotides to focal adhesion kinase (FAK) and a dominant-negative mutant of ras (S17Nras), we found that both FAK and c-Ras were required for the activation of matrix metalloproteinase 9 secretion by FN. In addition, both antisense oligonucleotides to FAK and S17Nras inhibited mitogen-activated protein kinase activation by FN treatment, suggesting the involvement of mitogenactivated protein kinase in the FN-dependent signaling.

Original languageEnglish
Pages (from-to)900-903
Number of pages4
JournalCancer Research
Volume58
Issue number5
Publication statusPublished - Mar 1 1998

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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