Both amacrine and bipolar cells release glutamate in the rat retina after ischemia/reperfusion insult in vitro

Yinghua Du, Kazuyuki Hirooka, Osamu Miyamoto, Toshifumi Itano, Masaaki Tokuda, Fumio Shiraga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: To investigate which cells in the inner nuclear layer release glutamate after exposure through the use of a model mimicking rat retina ischemia/reperfusion induced by glucose/oxygen deprivation in vitro. Methods: An in vitro retinal ischemia model was used to monitor the release of glutamate by staining with diaminobenzidine hydrochloride. Immunocytochemistry was used to identify the cells releasing glutamate during ischemic/reperfusion injury. Results: On immunocytochemistry, double-labeling of some amacrine and bipolar cells was observed, with somata being stained blue by GABA and two portions of the processes labeled brown due to glutamate reactivity. Some somata of amacrine cells were double-labeled with calbindin, while horizontal cells were single-labeled with calbindin. Conclusions: During ischemia/reperfusion injury in vitro, both amacrine and bipolar cells release glutamate. These results may be related to the patterns of apoptotic cell death seen in the inner retina.

Original languageEnglish
Pages (from-to)782-788
Number of pages7
JournalCurrent Eye Research
Volume33
Issue number9
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

Fingerprint

Amacrine Cells
Reperfusion
Retina
Glutamic Acid
Ischemia
Calbindins
Carisoprodol
Reperfusion Injury
Immunohistochemistry
gamma-Aminobutyric Acid
Cell Death
In Vitro Techniques
Staining and Labeling
Oxygen
Glucose

Keywords

  • Amacrine cell
  • Bipolar cell
  • GABA
  • Glutamate
  • Immunocytochemistry
  • Ischemia

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Both amacrine and bipolar cells release glutamate in the rat retina after ischemia/reperfusion insult in vitro. / Du, Yinghua; Hirooka, Kazuyuki; Miyamoto, Osamu; Itano, Toshifumi; Tokuda, Masaaki; Shiraga, Fumio.

In: Current Eye Research, Vol. 33, No. 9, 09.2008, p. 782-788.

Research output: Contribution to journalArticle

Du, Yinghua ; Hirooka, Kazuyuki ; Miyamoto, Osamu ; Itano, Toshifumi ; Tokuda, Masaaki ; Shiraga, Fumio. / Both amacrine and bipolar cells release glutamate in the rat retina after ischemia/reperfusion insult in vitro. In: Current Eye Research. 2008 ; Vol. 33, No. 9. pp. 782-788.
@article{36630b8c921047eaa2e7b0310d230df6,
title = "Both amacrine and bipolar cells release glutamate in the rat retina after ischemia/reperfusion insult in vitro",
abstract = "Purpose: To investigate which cells in the inner nuclear layer release glutamate after exposure through the use of a model mimicking rat retina ischemia/reperfusion induced by glucose/oxygen deprivation in vitro. Methods: An in vitro retinal ischemia model was used to monitor the release of glutamate by staining with diaminobenzidine hydrochloride. Immunocytochemistry was used to identify the cells releasing glutamate during ischemic/reperfusion injury. Results: On immunocytochemistry, double-labeling of some amacrine and bipolar cells was observed, with somata being stained blue by GABA and two portions of the processes labeled brown due to glutamate reactivity. Some somata of amacrine cells were double-labeled with calbindin, while horizontal cells were single-labeled with calbindin. Conclusions: During ischemia/reperfusion injury in vitro, both amacrine and bipolar cells release glutamate. These results may be related to the patterns of apoptotic cell death seen in the inner retina.",
keywords = "Amacrine cell, Bipolar cell, GABA, Glutamate, Immunocytochemistry, Ischemia",
author = "Yinghua Du and Kazuyuki Hirooka and Osamu Miyamoto and Toshifumi Itano and Masaaki Tokuda and Fumio Shiraga",
year = "2008",
month = "9",
doi = "10.1080/02713680802334600",
language = "English",
volume = "33",
pages = "782--788",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "9",

}

TY - JOUR

T1 - Both amacrine and bipolar cells release glutamate in the rat retina after ischemia/reperfusion insult in vitro

AU - Du, Yinghua

AU - Hirooka, Kazuyuki

AU - Miyamoto, Osamu

AU - Itano, Toshifumi

AU - Tokuda, Masaaki

AU - Shiraga, Fumio

PY - 2008/9

Y1 - 2008/9

N2 - Purpose: To investigate which cells in the inner nuclear layer release glutamate after exposure through the use of a model mimicking rat retina ischemia/reperfusion induced by glucose/oxygen deprivation in vitro. Methods: An in vitro retinal ischemia model was used to monitor the release of glutamate by staining with diaminobenzidine hydrochloride. Immunocytochemistry was used to identify the cells releasing glutamate during ischemic/reperfusion injury. Results: On immunocytochemistry, double-labeling of some amacrine and bipolar cells was observed, with somata being stained blue by GABA and two portions of the processes labeled brown due to glutamate reactivity. Some somata of amacrine cells were double-labeled with calbindin, while horizontal cells were single-labeled with calbindin. Conclusions: During ischemia/reperfusion injury in vitro, both amacrine and bipolar cells release glutamate. These results may be related to the patterns of apoptotic cell death seen in the inner retina.

AB - Purpose: To investigate which cells in the inner nuclear layer release glutamate after exposure through the use of a model mimicking rat retina ischemia/reperfusion induced by glucose/oxygen deprivation in vitro. Methods: An in vitro retinal ischemia model was used to monitor the release of glutamate by staining with diaminobenzidine hydrochloride. Immunocytochemistry was used to identify the cells releasing glutamate during ischemic/reperfusion injury. Results: On immunocytochemistry, double-labeling of some amacrine and bipolar cells was observed, with somata being stained blue by GABA and two portions of the processes labeled brown due to glutamate reactivity. Some somata of amacrine cells were double-labeled with calbindin, while horizontal cells were single-labeled with calbindin. Conclusions: During ischemia/reperfusion injury in vitro, both amacrine and bipolar cells release glutamate. These results may be related to the patterns of apoptotic cell death seen in the inner retina.

KW - Amacrine cell

KW - Bipolar cell

KW - GABA

KW - Glutamate

KW - Immunocytochemistry

KW - Ischemia

UR - http://www.scopus.com/inward/record.url?scp=51949096075&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51949096075&partnerID=8YFLogxK

U2 - 10.1080/02713680802334600

DO - 10.1080/02713680802334600

M3 - Article

C2 - 18798081

AN - SCOPUS:51949096075

VL - 33

SP - 782

EP - 788

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 9

ER -