Bone regeneration potential of stem cells derived from periodontal ligament or gingival tissue sources encapsulated in RGD-modified alginate scaffold

Alireza Moshaverinia, Chider Chen, Xingtian Xu, Kentaro Akiyama, Sahar Ansari, Homayoun H. Zadeh, Songtao Shi

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) provide an advantageous alternative therapeutic option for bone regeneration in comparison to current treatment modalities. However, delivering MSCs to the defect site while maintaining a high MSC survival rate is still a critical challenge in MSC-mediated bone regeneration. Here, we tested the bone regeneration capacity of periodontal ligament stem cells (PDLSCs) and gingival mesenchymal stem cells (GMSCs) encapsulated in a novel RGD-(arginine-glycine-aspartic acid tripeptide) coupled alginate microencapsulation system in vitro and in vivo. Five-millimeter- diameter critical-size calvarial defects were created in immunocompromised mice and PDLSCs and GMSCs encapsulated in RGD-modified alginate microspheres were transplanted into the defect sites. New bone formation was assessed using microcomputed tomography and histological analyses 8 weeks after transplantation. Results confirmed that our microencapsulation system significantly enhanced MSC viability and osteogenic differentiation in vitro compared with non-RGD-containing alginate hydrogel microspheres with larger diameters. Results confirmed that PDLSCs were able to repair the calvarial defects by promoting the formation of mineralized tissue, while GMSCs showed significantly lower osteogenic differentiation capability. Further, results revealed that RGD-coupled alginate scaffold facilitated the differentiation of oral MSCs toward an osteoblast lineage in vitro and in vivo, as assessed by expression of osteogenic markers Runx2, ALP, and osteocalcin. In conclusion, these results for the first time demonstrated that MSCs derived from orofacial tissue encapsulated in RGD-modified alginate scaffold show promise for craniofacial bone regeneration. This treatment modality has many potential dental and orthopedic applications.

Original languageEnglish
Pages (from-to)611-621
Number of pages11
JournalTissue Engineering - Part A
Volume20
Issue number3-4
DOIs
Publication statusPublished - Feb 1 2014
Externally publishedYes

Fingerprint

Periodontal Ligament
Bone Regeneration
Ligaments
Alginate
Stem cells
Mesenchymal Stromal Cells
Scaffolds
Bone
Stem Cells
Tissue
Drug Compounding
Microencapsulation
Microspheres
Defects
Cell Survival
alginic acid
X-Ray Microtomography
Hydrogel
Osteocalcin
Osteoblasts

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Bone regeneration potential of stem cells derived from periodontal ligament or gingival tissue sources encapsulated in RGD-modified alginate scaffold. / Moshaverinia, Alireza; Chen, Chider; Xu, Xingtian; Akiyama, Kentaro; Ansari, Sahar; Zadeh, Homayoun H.; Shi, Songtao.

In: Tissue Engineering - Part A, Vol. 20, No. 3-4, 01.02.2014, p. 611-621.

Research output: Contribution to journalArticle

Moshaverinia, Alireza ; Chen, Chider ; Xu, Xingtian ; Akiyama, Kentaro ; Ansari, Sahar ; Zadeh, Homayoun H. ; Shi, Songtao. / Bone regeneration potential of stem cells derived from periodontal ligament or gingival tissue sources encapsulated in RGD-modified alginate scaffold. In: Tissue Engineering - Part A. 2014 ; Vol. 20, No. 3-4. pp. 611-621.
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