Bonding effectiveness and interfacial characterization of a HEMA/TEGDMA-free three-step etch&rinse adhesive

Atsushi Mine, Jan De Munck, Kirsten L. Van Landuyt, André Poitevin, Takuo Kuboki, Yasuhiro Yoshida, Kazuomi Suzuki, Paul Lambrechts, Bart Van Meerbeek

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives: Small and hydrophilic monomers as HEMA and TEGDMA can easily penetrate human tissues. For biocompatibility issues it is therefore better to avoid such monomers in dental adhesive formulations. The purpose of this study was (1) to determine the micro-tensile bond strength (μTBS) to enamel/dentin of a HEMA/TEGDMA-free three-step etch&rinse adhesive (cmf Adhesive System, Saremco), and (2) to characterize it's interfacial interaction with enamel/dentin using transmission electron microscopy (TEM). Methods: Twelve intact freshly extracted human third molars were used for μTBS. The three-step etch&rinse adhesive OptiBond FL (Kerr) served as control adhesive. After bonding procedures and subsequent composite build-up (Z100, 3M ESPE), specimens were sectioned to sticks and trimmed at the interface to a cylindrical hour-glass shape. TEM section of non-demineralized and demineralized sections were prepared by ultra-microtomy and examined. Results: The μTBS of the cmf Adhesive System to enamel was not statistically significantly different from that of the control (OptiBond FL) adhesive (p = 0.3512); the μTBS to dentin was however significantly lower (p <0.0001). TEM showed a tight interface at enamel for both adhesives. A relatively thick, completely demineralized and acid-resistant hybrid layer was formed at dentin. Some degree of spot- and cluster-like nano-leakage was observed with cmf Adhesive System, but did not differ in extent and form in comparison to that observed with the OptiBond FL control. Conclusions: The new three-step adhesive cmf Adhesive System showed indications of good bonding effectiveness, certainly to enamel and only significantly lower to dentin, when compared to the gold-standard adhesive. The latter may be related to the adapted HEMA/TEGDMA-free formulation and the somewhat lower mechanical properties.

Original languageEnglish
Pages (from-to)767-773
Number of pages7
JournalJournal of Dentistry
Volume36
Issue number10
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Adhesives
Dentin
Dental Enamel
Tensile Strength
Transmission Electron Microscopy
Microtomy
Dental Cements
Third Molar
hydroxyethyl methacrylate
triethylene glycol dimethacrylate
Gold
Glass
Acids
OptiBond FL

Keywords

  • Adhesion
  • Allergy
  • Bond strength
  • Dentin
  • Enamel
  • Etch&rinse
  • HEMA-free
  • TEGDMA-free
  • TEM
  • Three-step adhesive

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Bonding effectiveness and interfacial characterization of a HEMA/TEGDMA-free three-step etch&rinse adhesive. / Mine, Atsushi; De Munck, Jan; Van Landuyt, Kirsten L.; Poitevin, André; Kuboki, Takuo; Yoshida, Yasuhiro; Suzuki, Kazuomi; Lambrechts, Paul; Van Meerbeek, Bart.

In: Journal of Dentistry, Vol. 36, No. 10, 10.2008, p. 767-773.

Research output: Contribution to journalArticle

Mine, A, De Munck, J, Van Landuyt, KL, Poitevin, A, Kuboki, T, Yoshida, Y, Suzuki, K, Lambrechts, P & Van Meerbeek, B 2008, 'Bonding effectiveness and interfacial characterization of a HEMA/TEGDMA-free three-step etch&rinse adhesive', Journal of Dentistry, vol. 36, no. 10, pp. 767-773. https://doi.org/10.1016/j.jdent.2008.05.012
Mine, Atsushi ; De Munck, Jan ; Van Landuyt, Kirsten L. ; Poitevin, André ; Kuboki, Takuo ; Yoshida, Yasuhiro ; Suzuki, Kazuomi ; Lambrechts, Paul ; Van Meerbeek, Bart. / Bonding effectiveness and interfacial characterization of a HEMA/TEGDMA-free three-step etch&rinse adhesive. In: Journal of Dentistry. 2008 ; Vol. 36, No. 10. pp. 767-773.
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abstract = "Objectives: Small and hydrophilic monomers as HEMA and TEGDMA can easily penetrate human tissues. For biocompatibility issues it is therefore better to avoid such monomers in dental adhesive formulations. The purpose of this study was (1) to determine the micro-tensile bond strength (μTBS) to enamel/dentin of a HEMA/TEGDMA-free three-step etch&rinse adhesive (cmf Adhesive System, Saremco), and (2) to characterize it's interfacial interaction with enamel/dentin using transmission electron microscopy (TEM). Methods: Twelve intact freshly extracted human third molars were used for μTBS. The three-step etch&rinse adhesive OptiBond FL (Kerr) served as control adhesive. After bonding procedures and subsequent composite build-up (Z100, 3M ESPE), specimens were sectioned to sticks and trimmed at the interface to a cylindrical hour-glass shape. TEM section of non-demineralized and demineralized sections were prepared by ultra-microtomy and examined. Results: The μTBS of the cmf Adhesive System to enamel was not statistically significantly different from that of the control (OptiBond FL) adhesive (p = 0.3512); the μTBS to dentin was however significantly lower (p <0.0001). TEM showed a tight interface at enamel for both adhesives. A relatively thick, completely demineralized and acid-resistant hybrid layer was formed at dentin. Some degree of spot- and cluster-like nano-leakage was observed with cmf Adhesive System, but did not differ in extent and form in comparison to that observed with the OptiBond FL control. Conclusions: The new three-step adhesive cmf Adhesive System showed indications of good bonding effectiveness, certainly to enamel and only significantly lower to dentin, when compared to the gold-standard adhesive. The latter may be related to the adapted HEMA/TEGDMA-free formulation and the somewhat lower mechanical properties.",
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AU - Poitevin, André

AU - Kuboki, Takuo

AU - Yoshida, Yasuhiro

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N2 - Objectives: Small and hydrophilic monomers as HEMA and TEGDMA can easily penetrate human tissues. For biocompatibility issues it is therefore better to avoid such monomers in dental adhesive formulations. The purpose of this study was (1) to determine the micro-tensile bond strength (μTBS) to enamel/dentin of a HEMA/TEGDMA-free three-step etch&rinse adhesive (cmf Adhesive System, Saremco), and (2) to characterize it's interfacial interaction with enamel/dentin using transmission electron microscopy (TEM). Methods: Twelve intact freshly extracted human third molars were used for μTBS. The three-step etch&rinse adhesive OptiBond FL (Kerr) served as control adhesive. After bonding procedures and subsequent composite build-up (Z100, 3M ESPE), specimens were sectioned to sticks and trimmed at the interface to a cylindrical hour-glass shape. TEM section of non-demineralized and demineralized sections were prepared by ultra-microtomy and examined. Results: The μTBS of the cmf Adhesive System to enamel was not statistically significantly different from that of the control (OptiBond FL) adhesive (p = 0.3512); the μTBS to dentin was however significantly lower (p <0.0001). TEM showed a tight interface at enamel for both adhesives. A relatively thick, completely demineralized and acid-resistant hybrid layer was formed at dentin. Some degree of spot- and cluster-like nano-leakage was observed with cmf Adhesive System, but did not differ in extent and form in comparison to that observed with the OptiBond FL control. Conclusions: The new three-step adhesive cmf Adhesive System showed indications of good bonding effectiveness, certainly to enamel and only significantly lower to dentin, when compared to the gold-standard adhesive. The latter may be related to the adapted HEMA/TEGDMA-free formulation and the somewhat lower mechanical properties.

AB - Objectives: Small and hydrophilic monomers as HEMA and TEGDMA can easily penetrate human tissues. For biocompatibility issues it is therefore better to avoid such monomers in dental adhesive formulations. The purpose of this study was (1) to determine the micro-tensile bond strength (μTBS) to enamel/dentin of a HEMA/TEGDMA-free three-step etch&rinse adhesive (cmf Adhesive System, Saremco), and (2) to characterize it's interfacial interaction with enamel/dentin using transmission electron microscopy (TEM). Methods: Twelve intact freshly extracted human third molars were used for μTBS. The three-step etch&rinse adhesive OptiBond FL (Kerr) served as control adhesive. After bonding procedures and subsequent composite build-up (Z100, 3M ESPE), specimens were sectioned to sticks and trimmed at the interface to a cylindrical hour-glass shape. TEM section of non-demineralized and demineralized sections were prepared by ultra-microtomy and examined. Results: The μTBS of the cmf Adhesive System to enamel was not statistically significantly different from that of the control (OptiBond FL) adhesive (p = 0.3512); the μTBS to dentin was however significantly lower (p <0.0001). TEM showed a tight interface at enamel for both adhesives. A relatively thick, completely demineralized and acid-resistant hybrid layer was formed at dentin. Some degree of spot- and cluster-like nano-leakage was observed with cmf Adhesive System, but did not differ in extent and form in comparison to that observed with the OptiBond FL control. Conclusions: The new three-step adhesive cmf Adhesive System showed indications of good bonding effectiveness, certainly to enamel and only significantly lower to dentin, when compared to the gold-standard adhesive. The latter may be related to the adapted HEMA/TEGDMA-free formulation and the somewhat lower mechanical properties.

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