Boar seminal plasma or hen's egg yolk decrease the in-vitro chemotactic and phagocytotic activities of neutrophils when co-incubated with boar or bull sperm

J. C. Li, S. Yamaguchi, Hiroaki Funahashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The objective was to determine the effects of boar seminal plasma and hen's egg yolk on chemotaxis and phagocytosis of porcine and bovine polymorphonuclear neutrophils (PMNs) in vitro. Chemotactic activity of PMNs was determined following culture for 90 min in a blind well chamber. Phagocytosis was assayed after co-culture of PMNs with sperm for 60 min. In the presence of ≥ 5% boar seminal plasma, chemotactic activity of PMNs was reduced (P <0.05) in both pigs (from 1126.1 to 934.2-1009.1 cells/mm 2) and in cows (from 1067.1 to 768.9-800.0 cells/mm 2). Furthermore, ≥ 5% boar seminal plasma reduced (P <0.05) leukocyte phagocytosis in pigs (26.2-32.1%) and cows (27.2-30.0%) compared to controls (41.7 and 42.1%, respectively). Although 20% hen's egg yolk increased (P <0.05) chemotactic activity of PMNs in pigs (from 790.4 to 1006.1 cells/mm 2) and cows (from 789.9 to 953.5 cells/mm 2), egg yolk increased (P <0.05) phagocytotic activity of porcine PMNs (from 24.3 to 33.8%), but not the activity of bovine PMNs (15.1 vs 15.8% in controls). Boar seminal plasma and caffeine reduced (P <0.05) the egg yolk-induced increase in chemotaxis in both species (from 988.6 to 795.2 or 813.2 cells/mm 2 in pigs and from 953.5 to 779.4 or 833.8 cells/mm 2 in cows), and phagocytotic activities of PMN (from 33.8% to 15.2 or 13.3%) only in pigs (but not in cows; 11.2-15.1%). In conclusion, hen's egg yolk increased chemotactic activity of PMNs in both pigs and cows, whereas egg yolk increased only phagocytosis of PMNs in pigs, but not in cows. Even in the presence of egg yolk, boar seminal plasma and caffeine significantly reduced chemotactic activity of PMNs in pigs and cows, and phagocytotic activity of porcine PMNs.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalTheriogenology
Volume77
Issue number1
DOIs
Publication statusPublished - Jan 1 2012

Fingerprint

Egg Yolk
seminal plasma
Semen
boars
egg yolk
Spermatozoa
neutrophils
hens
bulls
Neutrophils
spermatozoa
Swine
swine
cows
phagocytosis
Phagocytosis
chemotaxis
cells
Chemotaxis
caffeine

Keywords

  • Boar seminal plasma
  • Cow
  • Egg yolk
  • Pig
  • PMN
  • Spermatozoa

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Equine
  • Food Animals
  • Small Animals

Cite this

@article{33da4f8811b74c9c95a3424a6fa1b2bb,
title = "Boar seminal plasma or hen's egg yolk decrease the in-vitro chemotactic and phagocytotic activities of neutrophils when co-incubated with boar or bull sperm",
abstract = "The objective was to determine the effects of boar seminal plasma and hen's egg yolk on chemotaxis and phagocytosis of porcine and bovine polymorphonuclear neutrophils (PMNs) in vitro. Chemotactic activity of PMNs was determined following culture for 90 min in a blind well chamber. Phagocytosis was assayed after co-culture of PMNs with sperm for 60 min. In the presence of ≥ 5{\%} boar seminal plasma, chemotactic activity of PMNs was reduced (P <0.05) in both pigs (from 1126.1 to 934.2-1009.1 cells/mm 2) and in cows (from 1067.1 to 768.9-800.0 cells/mm 2). Furthermore, ≥ 5{\%} boar seminal plasma reduced (P <0.05) leukocyte phagocytosis in pigs (26.2-32.1{\%}) and cows (27.2-30.0{\%}) compared to controls (41.7 and 42.1{\%}, respectively). Although 20{\%} hen's egg yolk increased (P <0.05) chemotactic activity of PMNs in pigs (from 790.4 to 1006.1 cells/mm 2) and cows (from 789.9 to 953.5 cells/mm 2), egg yolk increased (P <0.05) phagocytotic activity of porcine PMNs (from 24.3 to 33.8{\%}), but not the activity of bovine PMNs (15.1 vs 15.8{\%} in controls). Boar seminal plasma and caffeine reduced (P <0.05) the egg yolk-induced increase in chemotaxis in both species (from 988.6 to 795.2 or 813.2 cells/mm 2 in pigs and from 953.5 to 779.4 or 833.8 cells/mm 2 in cows), and phagocytotic activities of PMN (from 33.8{\%} to 15.2 or 13.3{\%}) only in pigs (but not in cows; 11.2-15.1{\%}). In conclusion, hen's egg yolk increased chemotactic activity of PMNs in both pigs and cows, whereas egg yolk increased only phagocytosis of PMNs in pigs, but not in cows. Even in the presence of egg yolk, boar seminal plasma and caffeine significantly reduced chemotactic activity of PMNs in pigs and cows, and phagocytotic activity of porcine PMNs.",
keywords = "Boar seminal plasma, Cow, Egg yolk, Pig, PMN, Spermatozoa",
author = "Li, {J. C.} and S. Yamaguchi and Hiroaki Funahashi",
year = "2012",
month = "1",
day = "1",
doi = "10.1016/j.theriogenology.2011.07.018",
language = "English",
volume = "77",
pages = "73--80",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Boar seminal plasma or hen's egg yolk decrease the in-vitro chemotactic and phagocytotic activities of neutrophils when co-incubated with boar or bull sperm

AU - Li, J. C.

AU - Yamaguchi, S.

AU - Funahashi, Hiroaki

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The objective was to determine the effects of boar seminal plasma and hen's egg yolk on chemotaxis and phagocytosis of porcine and bovine polymorphonuclear neutrophils (PMNs) in vitro. Chemotactic activity of PMNs was determined following culture for 90 min in a blind well chamber. Phagocytosis was assayed after co-culture of PMNs with sperm for 60 min. In the presence of ≥ 5% boar seminal plasma, chemotactic activity of PMNs was reduced (P <0.05) in both pigs (from 1126.1 to 934.2-1009.1 cells/mm 2) and in cows (from 1067.1 to 768.9-800.0 cells/mm 2). Furthermore, ≥ 5% boar seminal plasma reduced (P <0.05) leukocyte phagocytosis in pigs (26.2-32.1%) and cows (27.2-30.0%) compared to controls (41.7 and 42.1%, respectively). Although 20% hen's egg yolk increased (P <0.05) chemotactic activity of PMNs in pigs (from 790.4 to 1006.1 cells/mm 2) and cows (from 789.9 to 953.5 cells/mm 2), egg yolk increased (P <0.05) phagocytotic activity of porcine PMNs (from 24.3 to 33.8%), but not the activity of bovine PMNs (15.1 vs 15.8% in controls). Boar seminal plasma and caffeine reduced (P <0.05) the egg yolk-induced increase in chemotaxis in both species (from 988.6 to 795.2 or 813.2 cells/mm 2 in pigs and from 953.5 to 779.4 or 833.8 cells/mm 2 in cows), and phagocytotic activities of PMN (from 33.8% to 15.2 or 13.3%) only in pigs (but not in cows; 11.2-15.1%). In conclusion, hen's egg yolk increased chemotactic activity of PMNs in both pigs and cows, whereas egg yolk increased only phagocytosis of PMNs in pigs, but not in cows. Even in the presence of egg yolk, boar seminal plasma and caffeine significantly reduced chemotactic activity of PMNs in pigs and cows, and phagocytotic activity of porcine PMNs.

AB - The objective was to determine the effects of boar seminal plasma and hen's egg yolk on chemotaxis and phagocytosis of porcine and bovine polymorphonuclear neutrophils (PMNs) in vitro. Chemotactic activity of PMNs was determined following culture for 90 min in a blind well chamber. Phagocytosis was assayed after co-culture of PMNs with sperm for 60 min. In the presence of ≥ 5% boar seminal plasma, chemotactic activity of PMNs was reduced (P <0.05) in both pigs (from 1126.1 to 934.2-1009.1 cells/mm 2) and in cows (from 1067.1 to 768.9-800.0 cells/mm 2). Furthermore, ≥ 5% boar seminal plasma reduced (P <0.05) leukocyte phagocytosis in pigs (26.2-32.1%) and cows (27.2-30.0%) compared to controls (41.7 and 42.1%, respectively). Although 20% hen's egg yolk increased (P <0.05) chemotactic activity of PMNs in pigs (from 790.4 to 1006.1 cells/mm 2) and cows (from 789.9 to 953.5 cells/mm 2), egg yolk increased (P <0.05) phagocytotic activity of porcine PMNs (from 24.3 to 33.8%), but not the activity of bovine PMNs (15.1 vs 15.8% in controls). Boar seminal plasma and caffeine reduced (P <0.05) the egg yolk-induced increase in chemotaxis in both species (from 988.6 to 795.2 or 813.2 cells/mm 2 in pigs and from 953.5 to 779.4 or 833.8 cells/mm 2 in cows), and phagocytotic activities of PMN (from 33.8% to 15.2 or 13.3%) only in pigs (but not in cows; 11.2-15.1%). In conclusion, hen's egg yolk increased chemotactic activity of PMNs in both pigs and cows, whereas egg yolk increased only phagocytosis of PMNs in pigs, but not in cows. Even in the presence of egg yolk, boar seminal plasma and caffeine significantly reduced chemotactic activity of PMNs in pigs and cows, and phagocytotic activity of porcine PMNs.

KW - Boar seminal plasma

KW - Cow

KW - Egg yolk

KW - Pig

KW - PMN

KW - Spermatozoa

UR - http://www.scopus.com/inward/record.url?scp=83055181337&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=83055181337&partnerID=8YFLogxK

U2 - 10.1016/j.theriogenology.2011.07.018

DO - 10.1016/j.theriogenology.2011.07.018

M3 - Article

C2 - 21872309

AN - SCOPUS:83055181337

VL - 77

SP - 73

EP - 80

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

IS - 1

ER -