Blue light exposure and nutrient conditions influence the expression of genes involved in simultaneous hyphal knot formation in Coprinopsis cinerea

Yuichi Sakamoto, Shiho Sato, Miyuki Ito, Yuki Ando, Kiyoshi Nakahori, Hajime Muraguchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Light and nutrients are crucial environmental factors influencing fungal sexual reproduction. Blue light induces simultaneous hyphal knot formation in Coprinopsis cinerea mycelia grown on low-glucose media but not in mycelia grown on high-glucose media. Many hyphal knots are visible in the arc near the edge of the colony one day after 15 min of blue light stimulation. These findings collectively suggest that blue light accelerates hyphal knot induction in nutrient-limited conditions. Transcriptome analysis revealed that gene expression after light exposure is divided into at least two major stages. In the first stage, genes coding for fasciclin (fas1), cyclopropane-fatty-acyl-phospholipid synthases (cfs1 and cfs2), and putative lipid exporter (nod1) are highly expressed after 1 h of light exposure in the mycelial region where the hyphal knot will be developed. These genes are upregulated by blue light and not influenced by glucose condition and mating. These results suggest that although some of the genes are critical for induction of the hyphal knots, they are not sufficient for hyphal knot development. In the second gene expression stage, genes encoding galectins (cgl1-3), farnesyl cysteine-carboxyl methyltransferases, mating pheromone-containing protein, nucleus protein (ich1), and laccase (lcc1) are specifically upregulated at 10–16 h after blue light exposure when the mycelia are cultivated on low-glucose media. These genes might be involved in the architecture of hyphal knots or signal transduction for further fruiting body development. These results contribute to the understanding of the effect of environmental factors on sexual reproduction in basidiomycetous fungi

Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalMicrobiological Research
Volume217
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Gene Expression
Light
Food
Mycelium
Glucose
Genes
Reproduction
Galectin 3
Laccase
Pheromones
Gene Expression Profiling
Signal Transduction
Phospholipids
Proteins
Fungi
Lipids

Keywords

  • Basidiomycete
  • Blue light
  • Coprinopsis cinerea
  • Fruiting body
  • Hyphal knot
  • Nutrient

ASJC Scopus subject areas

  • Microbiology

Cite this

Blue light exposure and nutrient conditions influence the expression of genes involved in simultaneous hyphal knot formation in Coprinopsis cinerea. / Sakamoto, Yuichi; Sato, Shiho; Ito, Miyuki; Ando, Yuki; Nakahori, Kiyoshi; Muraguchi, Hajime.

In: Microbiological Research, Vol. 217, 01.12.2018, p. 81-90.

Research output: Contribution to journalArticle

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