blaOXA-731, a new chromosome-encoded blaOXA-48-like variant in Shewanella sp. from the aquatic environment in Myanmar

Rathina Kumar Shanmugakani, Yo Sugawara, Yukihiro Akeda, Hideharu Hagiya, Noriko Sakamoto, Mya Mya Aye, Thuzar Myint, Shigeyuki Hamada, Kazunori Tomono

Research output: Contribution to journalArticlepeer-review

Abstract

Shewanella sp., the progenitors of blaOXA-48-like genes are increasingly reported with the possession of different blaOXA-48-like variants. This study aims to characterize blaOXA-731, a new variant of a blaOXA-48-like gene identified in Shewanella sp. isolated from the aquatic environment in Myanmar. Phylogenetic analysis of the blaOXA-731 sequence with other blaOXA-48-like variants showed that it has the highest nucleotide identity of 86.09% with blaOXA-48. However, the active site motifs in OXA-731 were 100% identical to that in OXA-48. Whole-genome sequencing analysis showed that blaOXA-731 is not surrounded by any mobile genetic elements. The genetic context of blaOXA-731 was found as similar to other blaOXA-48-like genes previously identified in Shewanella sp. S1 nuclease pulsed-field gel electrophoresis followed by Southern blotting confirmed the location of blaOXA-731 in the chromosome of the Shewanella genome. Cloning and expression studies showed that OXA-731 has β-lactamase activity similar to OXA-48 and OXA-181, but it has no significant carbapenemase activity. Our results showed the significance of blaOXA-48-like-carrying Shewanella sp. in the spreading of blaOXA-48-like genes in the community.

Original languageEnglish
Pages (from-to)548-554
Number of pages7
JournalEnvironmental Microbiology Reports
Volume12
Issue number5
DOIs
Publication statusPublished - Oct 1 2020

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)

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