TY - JOUR
T1 - blaOXA-731, a new chromosome-encoded blaOXA-48-like variant in Shewanella sp. from the aquatic environment in Myanmar
AU - Shanmugakani, Rathina Kumar
AU - Sugawara, Yo
AU - Akeda, Yukihiro
AU - Hagiya, Hideharu
AU - Sakamoto, Noriko
AU - Aye, Mya Mya
AU - Myint, Thuzar
AU - Hamada, Shigeyuki
AU - Tomono, Kazunori
N1 - Funding Information:
We thank Dr. Takuya Kodera and Dr. Mitsuo Kawase from Tohoku Bio-Array, Sendai, Japan for their support in the manufacturing of the dipsticks and related reagents. We acknowledge the NGS core facility of Genome Information Research Centre at the Research Institute for Microbial Diseases, Osaka University, Osaka, Japan for their support in DNA sequencing. We thank Yumi Sasaki and Kazuhiro Maeda from Osaka University for their technical assistance on antimicrobial susceptibility testing. We appreciate Isao Nishi and Aki Ueda from Osaka University for their help in performing MALDI-TOF MS analysis. This work was supported by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) from the Ministry of Education, Culture, Sport, Science and Technology in Japan and the Japan Agency for Medical Research and Development (AMED).
Funding Information:
We thank Dr. Takuya Kodera and Dr. Mitsuo Kawase from Tohoku Bio‐Array, Sendai, Japan for their support in the manufacturing of the dipsticks and related reagents. We acknowledge the NGS core facility of Genome Information Research Centre at the Research Institute for Microbial Diseases, Osaka University, Osaka, Japan for their support in DNA sequencing. We thank Yumi Sasaki and Kazuhiro Maeda from Osaka University for their technical assistance on antimicrobial susceptibility testing. We appreciate Isao Nishi and Aki Ueda from Osaka University for their help in performing MALDI‐TOF MS analysis. This work was supported by the Japan Initiative for Global Research Network on Infectious Diseases (J‐GRID) from the Ministry of Education, Culture, Sport, Science and Technology in Japan and the Japan Agency for Medical Research and Development (AMED).
Publisher Copyright:
© 2020 Society for Applied Microbiology and John Wiley & Sons Ltd
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Shewanella sp., the progenitors of blaOXA-48-like genes are increasingly reported with the possession of different blaOXA-48-like variants. This study aims to characterize blaOXA-731, a new variant of a blaOXA-48-like gene identified in Shewanella sp. isolated from the aquatic environment in Myanmar. Phylogenetic analysis of the blaOXA-731 sequence with other blaOXA-48-like variants showed that it has the highest nucleotide identity of 86.09% with blaOXA-48. However, the active site motifs in OXA-731 were 100% identical to that in OXA-48. Whole-genome sequencing analysis showed that blaOXA-731 is not surrounded by any mobile genetic elements. The genetic context of blaOXA-731 was found as similar to other blaOXA-48-like genes previously identified in Shewanella sp. S1 nuclease pulsed-field gel electrophoresis followed by Southern blotting confirmed the location of blaOXA-731 in the chromosome of the Shewanella genome. Cloning and expression studies showed that OXA-731 has β-lactamase activity similar to OXA-48 and OXA-181, but it has no significant carbapenemase activity. Our results showed the significance of blaOXA-48-like-carrying Shewanella sp. in the spreading of blaOXA-48-like genes in the community.
AB - Shewanella sp., the progenitors of blaOXA-48-like genes are increasingly reported with the possession of different blaOXA-48-like variants. This study aims to characterize blaOXA-731, a new variant of a blaOXA-48-like gene identified in Shewanella sp. isolated from the aquatic environment in Myanmar. Phylogenetic analysis of the blaOXA-731 sequence with other blaOXA-48-like variants showed that it has the highest nucleotide identity of 86.09% with blaOXA-48. However, the active site motifs in OXA-731 were 100% identical to that in OXA-48. Whole-genome sequencing analysis showed that blaOXA-731 is not surrounded by any mobile genetic elements. The genetic context of blaOXA-731 was found as similar to other blaOXA-48-like genes previously identified in Shewanella sp. S1 nuclease pulsed-field gel electrophoresis followed by Southern blotting confirmed the location of blaOXA-731 in the chromosome of the Shewanella genome. Cloning and expression studies showed that OXA-731 has β-lactamase activity similar to OXA-48 and OXA-181, but it has no significant carbapenemase activity. Our results showed the significance of blaOXA-48-like-carrying Shewanella sp. in the spreading of blaOXA-48-like genes in the community.
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U2 - 10.1111/1758-2229.12875
DO - 10.1111/1758-2229.12875
M3 - Article
C2 - 32776437
AN - SCOPUS:85090310000
SN - 1758-2229
VL - 12
SP - 548
EP - 554
JO - Environmental Microbiology Reports
JF - Environmental Microbiology Reports
IS - 5
ER -