Bisphenol A stimulates NO synthesis through a non-genomic estrogen receptor-mediated mechanism in mouse endothelial cells

Soichi Noguchi, Mikiya Nakatsuka, Kazuo Asagiri, Toshihiro Habara, Masayo Takata, Hideki Konishi, Takafumi Kudo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Biological actions of bisphenol A (BPA), an environmental chemical, have not been fully elucidated. We studied effect of BPA on nitric oxide (NO) synthesis in the murine endothelial cell line, MSS31. BPA (1-100 μM) increased nitrite/nitrate, a stable metabolites of NO, levels in culture medium of MSS31. However, Western blotting showed that the level of endothelial NO synthase protein was not increased by 16 h of treatment with BPA (10 μM). ICI 182,780 (10 μM), an estrogen receptor (ER) antagonist, suppressed BPA-induced NO synthesis while actinomycin D (1 μg/ml), a transcription inhibitor, or cycloheximide (40 μM), a protein synthesis inhibitor, exhibited no effect on BPA-induced NO synthesis. These results indicate that BPA stimulates NO synthesis through a non-genomic ER-mediated mechanism. Short-term effects of BPA on NO synthesis were weak but similar to 17β-estradiol.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalToxicology Letters
Volume135
Issue number1-2
DOIs
Publication statusPublished - Sep 5 2002

Fingerprint

Endothelial cells
Estrogen Receptors
Nitric Oxide
Endothelial Cells
Protein Synthesis Inhibitors
bisphenol A
Nitric Oxide Synthase Type III
Transcription
Cycloheximide
Metabolites
Nitrites
Nitrates
Culture Media
Estradiol
Western Blotting
Cell Line

Keywords

  • Bisphenol A
  • Endothelial cell
  • Estrogen
  • Nitric oxide
  • Xenoestrogen

ASJC Scopus subject areas

  • Toxicology

Cite this

Bisphenol A stimulates NO synthesis through a non-genomic estrogen receptor-mediated mechanism in mouse endothelial cells. / Noguchi, Soichi; Nakatsuka, Mikiya; Asagiri, Kazuo; Habara, Toshihiro; Takata, Masayo; Konishi, Hideki; Kudo, Takafumi.

In: Toxicology Letters, Vol. 135, No. 1-2, 05.09.2002, p. 95-101.

Research output: Contribution to journalArticle

Noguchi, Soichi ; Nakatsuka, Mikiya ; Asagiri, Kazuo ; Habara, Toshihiro ; Takata, Masayo ; Konishi, Hideki ; Kudo, Takafumi. / Bisphenol A stimulates NO synthesis through a non-genomic estrogen receptor-mediated mechanism in mouse endothelial cells. In: Toxicology Letters. 2002 ; Vol. 135, No. 1-2. pp. 95-101.
@article{d0fc3eecfcd54c17ae0bf1c9b63adf24,
title = "Bisphenol A stimulates NO synthesis through a non-genomic estrogen receptor-mediated mechanism in mouse endothelial cells",
abstract = "Biological actions of bisphenol A (BPA), an environmental chemical, have not been fully elucidated. We studied effect of BPA on nitric oxide (NO) synthesis in the murine endothelial cell line, MSS31. BPA (1-100 μM) increased nitrite/nitrate, a stable metabolites of NO, levels in culture medium of MSS31. However, Western blotting showed that the level of endothelial NO synthase protein was not increased by 16 h of treatment with BPA (10 μM). ICI 182,780 (10 μM), an estrogen receptor (ER) antagonist, suppressed BPA-induced NO synthesis while actinomycin D (1 μg/ml), a transcription inhibitor, or cycloheximide (40 μM), a protein synthesis inhibitor, exhibited no effect on BPA-induced NO synthesis. These results indicate that BPA stimulates NO synthesis through a non-genomic ER-mediated mechanism. Short-term effects of BPA on NO synthesis were weak but similar to 17β-estradiol.",
keywords = "Bisphenol A, Endothelial cell, Estrogen, Nitric oxide, Xenoestrogen",
author = "Soichi Noguchi and Mikiya Nakatsuka and Kazuo Asagiri and Toshihiro Habara and Masayo Takata and Hideki Konishi and Takafumi Kudo",
year = "2002",
month = "9",
day = "5",
doi = "10.1016/S0378-4274(02)00252-7",
language = "English",
volume = "135",
pages = "95--101",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier BV",
number = "1-2",

}

TY - JOUR

T1 - Bisphenol A stimulates NO synthesis through a non-genomic estrogen receptor-mediated mechanism in mouse endothelial cells

AU - Noguchi, Soichi

AU - Nakatsuka, Mikiya

AU - Asagiri, Kazuo

AU - Habara, Toshihiro

AU - Takata, Masayo

AU - Konishi, Hideki

AU - Kudo, Takafumi

PY - 2002/9/5

Y1 - 2002/9/5

N2 - Biological actions of bisphenol A (BPA), an environmental chemical, have not been fully elucidated. We studied effect of BPA on nitric oxide (NO) synthesis in the murine endothelial cell line, MSS31. BPA (1-100 μM) increased nitrite/nitrate, a stable metabolites of NO, levels in culture medium of MSS31. However, Western blotting showed that the level of endothelial NO synthase protein was not increased by 16 h of treatment with BPA (10 μM). ICI 182,780 (10 μM), an estrogen receptor (ER) antagonist, suppressed BPA-induced NO synthesis while actinomycin D (1 μg/ml), a transcription inhibitor, or cycloheximide (40 μM), a protein synthesis inhibitor, exhibited no effect on BPA-induced NO synthesis. These results indicate that BPA stimulates NO synthesis through a non-genomic ER-mediated mechanism. Short-term effects of BPA on NO synthesis were weak but similar to 17β-estradiol.

AB - Biological actions of bisphenol A (BPA), an environmental chemical, have not been fully elucidated. We studied effect of BPA on nitric oxide (NO) synthesis in the murine endothelial cell line, MSS31. BPA (1-100 μM) increased nitrite/nitrate, a stable metabolites of NO, levels in culture medium of MSS31. However, Western blotting showed that the level of endothelial NO synthase protein was not increased by 16 h of treatment with BPA (10 μM). ICI 182,780 (10 μM), an estrogen receptor (ER) antagonist, suppressed BPA-induced NO synthesis while actinomycin D (1 μg/ml), a transcription inhibitor, or cycloheximide (40 μM), a protein synthesis inhibitor, exhibited no effect on BPA-induced NO synthesis. These results indicate that BPA stimulates NO synthesis through a non-genomic ER-mediated mechanism. Short-term effects of BPA on NO synthesis were weak but similar to 17β-estradiol.

KW - Bisphenol A

KW - Endothelial cell

KW - Estrogen

KW - Nitric oxide

KW - Xenoestrogen

UR - http://www.scopus.com/inward/record.url?scp=0037026712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037026712&partnerID=8YFLogxK

U2 - 10.1016/S0378-4274(02)00252-7

DO - 10.1016/S0378-4274(02)00252-7

M3 - Article

VL - 135

SP - 95

EP - 101

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

IS - 1-2

ER -