Abstract
Biological actions of bisphenol A (BPA), an environmental chemical, have not been fully elucidated. We studied effect of BPA on nitric oxide (NO) synthesis in the murine endothelial cell line, MSS31. BPA (1-100 μM) increased nitrite/nitrate, a stable metabolites of NO, levels in culture medium of MSS31. However, Western blotting showed that the level of endothelial NO synthase protein was not increased by 16 h of treatment with BPA (10 μM). ICI 182,780 (10 μM), an estrogen receptor (ER) antagonist, suppressed BPA-induced NO synthesis while actinomycin D (1 μg/ml), a transcription inhibitor, or cycloheximide (40 μM), a protein synthesis inhibitor, exhibited no effect on BPA-induced NO synthesis. These results indicate that BPA stimulates NO synthesis through a non-genomic ER-mediated mechanism. Short-term effects of BPA on NO synthesis were weak but similar to 17β-estradiol.
| Original language | English |
|---|---|
| Pages (from-to) | 95-101 |
| Number of pages | 7 |
| Journal | Toxicology Letters |
| Volume | 135 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Sep 5 2002 |
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Keywords
- Bisphenol A
- Endothelial cell
- Estrogen
- Nitric oxide
- Xenoestrogen
ASJC Scopus subject areas
- Toxicology
Cite this
Bisphenol A stimulates NO synthesis through a non-genomic estrogen receptor-mediated mechanism in mouse endothelial cells. / Noguchi, Soichi; Nakatsuka, Mikiya; Asagiri, Kazuo; Habara, Toshihiro; Takata, Masayo; Konishi, Hideki; Kudo, Takafumi.
In: Toxicology Letters, Vol. 135, No. 1-2, 05.09.2002, p. 95-101.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bisphenol A stimulates NO synthesis through a non-genomic estrogen receptor-mediated mechanism in mouse endothelial cells
AU - Noguchi, Soichi
AU - Nakatsuka, Mikiya
AU - Asagiri, Kazuo
AU - Habara, Toshihiro
AU - Takata, Masayo
AU - Konishi, Hideki
AU - Kudo, Takafumi
PY - 2002/9/5
Y1 - 2002/9/5
N2 - Biological actions of bisphenol A (BPA), an environmental chemical, have not been fully elucidated. We studied effect of BPA on nitric oxide (NO) synthesis in the murine endothelial cell line, MSS31. BPA (1-100 μM) increased nitrite/nitrate, a stable metabolites of NO, levels in culture medium of MSS31. However, Western blotting showed that the level of endothelial NO synthase protein was not increased by 16 h of treatment with BPA (10 μM). ICI 182,780 (10 μM), an estrogen receptor (ER) antagonist, suppressed BPA-induced NO synthesis while actinomycin D (1 μg/ml), a transcription inhibitor, or cycloheximide (40 μM), a protein synthesis inhibitor, exhibited no effect on BPA-induced NO synthesis. These results indicate that BPA stimulates NO synthesis through a non-genomic ER-mediated mechanism. Short-term effects of BPA on NO synthesis were weak but similar to 17β-estradiol.
AB - Biological actions of bisphenol A (BPA), an environmental chemical, have not been fully elucidated. We studied effect of BPA on nitric oxide (NO) synthesis in the murine endothelial cell line, MSS31. BPA (1-100 μM) increased nitrite/nitrate, a stable metabolites of NO, levels in culture medium of MSS31. However, Western blotting showed that the level of endothelial NO synthase protein was not increased by 16 h of treatment with BPA (10 μM). ICI 182,780 (10 μM), an estrogen receptor (ER) antagonist, suppressed BPA-induced NO synthesis while actinomycin D (1 μg/ml), a transcription inhibitor, or cycloheximide (40 μM), a protein synthesis inhibitor, exhibited no effect on BPA-induced NO synthesis. These results indicate that BPA stimulates NO synthesis through a non-genomic ER-mediated mechanism. Short-term effects of BPA on NO synthesis were weak but similar to 17β-estradiol.
KW - Bisphenol A
KW - Endothelial cell
KW - Estrogen
KW - Nitric oxide
KW - Xenoestrogen
UR - http://www.scopus.com/inward/record.url?scp=0037026712&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037026712&partnerID=8YFLogxK
U2 - 10.1016/S0378-4274(02)00252-7
DO - 10.1016/S0378-4274(02)00252-7
M3 - Article
VL - 135
SP - 95
EP - 101
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 1-2
ER -