Abstract
Although chlorpromazine was shown to greatly inhibit a Ca2+-mediated cell death at favorable concentrations (10-6 ∼ 10-5 M), it caused a drastic decrease in cell viability at higher concentrations (10-4 ∼ 10-3 M) in a human neuroblastoma cell line. The toxic effect of chlorpromazine also occurred in Ca2+-free medium and was not parallel to the amount of thiobarbituric acid-reactive substances produced. These results indicate that chlorpromazine has biphasic effects on cell viability according to the concentrations added, i.e. a protective effect against cell damage caused by Ca2+, and a direct toxic effect independent of extracellular Ca2+ or of lipid peroxidation.
| Original language | English |
|---|---|
| Pages (from-to) | 335-339 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 71 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Nov 21 1986 |
| Externally published | Yes |
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Keywords
- Ca toxicity
- Cell viability
- Chlorpromazine
- Lipid peroxidation
- Neuroblastoma cell line
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Biphasic effects of chlorpromazine on cell viability in a neuroblastoma cell line. / Abe, Koji; Sekizawa, Tsuyoshi; Kogure, Kyuya.
In: Neuroscience Letters, Vol. 71, No. 3, 21.11.1986, p. 335-339.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biphasic effects of chlorpromazine on cell viability in a neuroblastoma cell line
AU - Abe, Koji
AU - Sekizawa, Tsuyoshi
AU - Kogure, Kyuya
PY - 1986/11/21
Y1 - 1986/11/21
N2 - Although chlorpromazine was shown to greatly inhibit a Ca2+-mediated cell death at favorable concentrations (10-6 ∼ 10-5 M), it caused a drastic decrease in cell viability at higher concentrations (10-4 ∼ 10-3 M) in a human neuroblastoma cell line. The toxic effect of chlorpromazine also occurred in Ca2+-free medium and was not parallel to the amount of thiobarbituric acid-reactive substances produced. These results indicate that chlorpromazine has biphasic effects on cell viability according to the concentrations added, i.e. a protective effect against cell damage caused by Ca2+, and a direct toxic effect independent of extracellular Ca2+ or of lipid peroxidation.
AB - Although chlorpromazine was shown to greatly inhibit a Ca2+-mediated cell death at favorable concentrations (10-6 ∼ 10-5 M), it caused a drastic decrease in cell viability at higher concentrations (10-4 ∼ 10-3 M) in a human neuroblastoma cell line. The toxic effect of chlorpromazine also occurred in Ca2+-free medium and was not parallel to the amount of thiobarbituric acid-reactive substances produced. These results indicate that chlorpromazine has biphasic effects on cell viability according to the concentrations added, i.e. a protective effect against cell damage caused by Ca2+, and a direct toxic effect independent of extracellular Ca2+ or of lipid peroxidation.
KW - Ca toxicity
KW - Cell viability
KW - Chlorpromazine
KW - Lipid peroxidation
KW - Neuroblastoma cell line
UR - http://www.scopus.com/inward/record.url?scp=0022977892&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022977892&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(86)90643-9
DO - 10.1016/0304-3940(86)90643-9
M3 - Article
C2 - 3796890
AN - SCOPUS:0022977892
VL - 71
SP - 335
EP - 339
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 3
ER -