Biosorption of metal elements by exopolymer nanofibrils excreted from Leptothrix cells

Tatsuki Kunoh, Makoto Nakanishi, Yoshihiro Kusano, Atsushi Itadani, Kota Ando, Syuji Matsumoto, Katsunori Tamura, Hitoshi Kunoh, Jun Takada

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Leptothrix species, aquatic Fe-oxidizing bacteria, excrete nano-scaled exopolymer fibrils. Once excreted, the fibrils weave together and coalesce to form extracellular, microtubular, immature sheaths encasing catenulate cells of Leptothrix. The immature sheaths, composed of aggregated nanofibrils with a homogeneous-looking matrix, attract and bind aqueous-phase inorganics, especially Fe, P, and Si, to form seemingly solid, mature sheaths of a hybrid organic–inorganic nature. To verify our assumption that the organic skeleton of the sheaths might sorb a broad range of other metallic and nonmetallic elements, we examined the sorption potential of chemically and enzymatically prepared protein-free organic sheath remnants for 47 available elements. The sheath remnants were found by XRF to sorb each of the 47 elements, although their sorption degree varied among the elements: >35% atomic percentages for Ti, Y, Zr, Ru, Rh, Ag, and Au. Electron microscopy, energy dispersive x-ray spectroscopy, electron and x-ray diffractions, and Fourier transform infrared spectroscopy analyses of sheath remnants that had sorbed Ag, Cu, and Pt revealed that (i) the sheath remnants comprised a 5–10 nm thick aggregation of fibrils, (ii) the test elements were distributed almost homogeneously throughout the fibrillar aggregate, (iii) the nanofibril matrix sorbing the elements was nearly amorphous, and (iv) these elements plausibly were bound to the matrix by ionic binding, especially via OH. The present results show that the constitutive protein-free exopolymer nanofibrils of the sheaths can contribute to creating novel filtering materials for recovering and recycling useful and/or hazardous elements from the environment.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalWater Research
Volume122
DOIs
Publication statusPublished - Oct 1 2017

Keywords

  • Amorphous organic–inorganic complex
  • Biosorption
  • Exopolymer fibrils
  • Leptothrix
  • Sheath materials
  • Sorbed metal elements

ASJC Scopus subject areas

  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

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    Kunoh, T., Nakanishi, M., Kusano, Y., Itadani, A., Ando, K., Matsumoto, S., Tamura, K., Kunoh, H., & Takada, J. (2017). Biosorption of metal elements by exopolymer nanofibrils excreted from Leptothrix cells. Water Research, 122, 139-147. https://doi.org/10.1016/j.watres.2017.05.003