Bioprecipitation of copper from a leaching solution by a moderately thermophilic iron-oxidizing bacterium

T. Sugio, K. Matsumoto, M. Takai, S. Wakasa, T. Sogawa, Kazuo Kamimura

Research output: Contribution to journalArticle

Abstract

A moderately thermophilic iron-oxidizing bacterium strain TI-1 produced 74 μmol H2S extracellularly when grown at 45°C for 15 days in 20 mL of ferrous sulfate-5AAG medium (pH 4.0) containing 1.0 % elemental sulfur and 0.31 % L-glutamic acid. Precipitation of copper from a leaching solution by the H2S produced by strain TI-1 was investigated. A leaching solution containg Cu2+ was prepared by the cultivation of Thiobacillus ferrooxidans AP19-3 on a copper concentrate (5.0 %)-medium (pH 2.5) for 15 days. When strain TI-1 was grown for 8 days in a ferrous sulfate-5AAG medium (pH 4.0) containing 1.0 % elemental sulfur, 0.31 % L-glutamic acid and 5 mM Cu2+ from a leaching solution, the Cu2+ in the medium was completely precipitated by the H2S produced by strain TI-1. Growth of strain TI-1 in ferrous sulfate-5AAG medium was not inhibited by 10 mM Cu2+. A moderately thermophilic sulfate-reducing bacterium Desulfotomaculum nigrificans IFO 136987 produced 45 μmol H2S extracellularly when grown at 45°C for 15 days in 20 ml of lactate (0.35 %)-medium. The maximum productivity of H2S by D. nigrificans was observed in the medium with an initial pH of 7.2. The bacterium did not produce H2S in the lactatemedium with a pH less than 6.0. Growth of D. nigrificans in the lactate-medium was completely inhibited by 0.56 mM Cu2+. These results suggest that strain TI-1 is superior to D. nigrificans in the ability to precipitate copper from the acidic leaching solution containing Cu2+.

Original languageEnglish
Pages (from-to)789-797
Number of pages9
JournalProcess Metallurgy
Volume9
Issue numberC
DOIs
Publication statusPublished - 1999

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Leaching
Bacteria
leaching
Iron
copper
Copper
iron
bacterium
sulfate
sulfur
thermophilic bacterium
Sulfur
acid
sulfate-reducing bacterium
Acids
Precipitates
productivity
Productivity
Sulfates

ASJC Scopus subject areas

  • Industrial and Manufacturing Engineering
  • Geotechnical Engineering and Engineering Geology

Cite this

Bioprecipitation of copper from a leaching solution by a moderately thermophilic iron-oxidizing bacterium. / Sugio, T.; Matsumoto, K.; Takai, M.; Wakasa, S.; Sogawa, T.; Kamimura, Kazuo.

In: Process Metallurgy, Vol. 9, No. C, 1999, p. 789-797.

Research output: Contribution to journalArticle

Sugio, T. ; Matsumoto, K. ; Takai, M. ; Wakasa, S. ; Sogawa, T. ; Kamimura, Kazuo. / Bioprecipitation of copper from a leaching solution by a moderately thermophilic iron-oxidizing bacterium. In: Process Metallurgy. 1999 ; Vol. 9, No. C. pp. 789-797.
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abstract = "A moderately thermophilic iron-oxidizing bacterium strain TI-1 produced 74 μmol H2S extracellularly when grown at 45°C for 15 days in 20 mL of ferrous sulfate-5AAG medium (pH 4.0) containing 1.0 {\%} elemental sulfur and 0.31 {\%} L-glutamic acid. Precipitation of copper from a leaching solution by the H2S produced by strain TI-1 was investigated. A leaching solution containg Cu2+ was prepared by the cultivation of Thiobacillus ferrooxidans AP19-3 on a copper concentrate (5.0 {\%})-medium (pH 2.5) for 15 days. When strain TI-1 was grown for 8 days in a ferrous sulfate-5AAG medium (pH 4.0) containing 1.0 {\%} elemental sulfur, 0.31 {\%} L-glutamic acid and 5 mM Cu2+ from a leaching solution, the Cu2+ in the medium was completely precipitated by the H2S produced by strain TI-1. Growth of strain TI-1 in ferrous sulfate-5AAG medium was not inhibited by 10 mM Cu2+. A moderately thermophilic sulfate-reducing bacterium Desulfotomaculum nigrificans IFO 136987 produced 45 μmol H2S extracellularly when grown at 45°C for 15 days in 20 ml of lactate (0.35 {\%})-medium. The maximum productivity of H2S by D. nigrificans was observed in the medium with an initial pH of 7.2. The bacterium did not produce H2S in the lactatemedium with a pH less than 6.0. Growth of D. nigrificans in the lactate-medium was completely inhibited by 0.56 mM Cu2+. These results suggest that strain TI-1 is superior to D. nigrificans in the ability to precipitate copper from the acidic leaching solution containing Cu2+.",
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