Biological control of crown gall of grapevine, rose, and tomato by nonpathogenic Agrobacterium vitis strain VAR03-1

A. Kawaguchi, K. Inoue, Yuki Ichinose

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30 Citations (Scopus)

Abstract

A nonpathogenic strain of Agrobacterium vitis VAR03-1 was tested as a biological control agent for crown gall of grapevine (Vitis vinifera). When roots of grapevine, rose (Rose multiflora), and tomato (Lycopersicon esculentum) were soaked in a cell suspension of antagonists before planting in soil infested with tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens, respectively, treatment with VAR03-1 significantly reduced the number of plants with tumors and disease severity in the three plant species. The inhibitory effects of treatment with VAR03-1 and the nonpathogenic A. rhizogenes strain K84 on crown gall of rose and tomato were almost identical, and the inhibitory effect of VAR03-1 on grapevine was superior to that of K84. Moreover, VAR03-1 greatly controlled crown gall of grapevine due to tumorigenic A. vitis in the field. VAR03-1 established populations averaging 106 colony forming units (CFU)/g of root in the rhizosphere of grapevine and persisted on roots for 2 years. VAR03-1 was bacteriocinogenic, producing a halo of inhibition against those three species of Agrobacterium. This is the first report that a nonpathogenic strain, VAR03-1, can effectively control crown gall caused by tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens.

Original languageEnglish
Pages (from-to)1218-1225
Number of pages8
JournalPhytopathology
Volume98
Issue number11
DOIs
Publication statusPublished - Nov 2008

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Rhizobium vitis
crown galls
biological control
Rosa
tomatoes
avirulent strains
Rosa multiflora
Rhizobium rhizogenes
Agrobacterium
Vitis vinifera
Solanum lycopersicum var. lycopersicum
disease severity
cell suspension culture
biological control agents
antagonists
rhizosphere
planting
neoplasms
soil

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Biological control of crown gall of grapevine, rose, and tomato by nonpathogenic Agrobacterium vitis strain VAR03-1. / Kawaguchi, A.; Inoue, K.; Ichinose, Yuki.

In: Phytopathology, Vol. 98, No. 11, 11.2008, p. 1218-1225.

Research output: Contribution to journalArticle

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