Biological activity of recombinant bovine interferon τ using an Autographa californica nuclear polyhedrosis virus expression system

Hitomi Takahashi, Shigeki Inumaru, Masashi Takahashi, Satoko Watanabe, Kosuke Iga, Yuichi Yokomizo, Masaya Geshi, Akira Okano, Kiyoshi Okuda

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7 Citations (Scopus)

Abstract

Bovine interferon (bIFN) τ which plays a key role in maternal-fetal recognition of pregnancy, was expressed by an Autographa californica nuclear polyhedrosis virus expression system. cDNA coding bIFNτ was derived from cultured trophoblast cells. The recombinant (r) bIFNτ had high antiviral activity (1 × 108 IU/mg) and the molecular weight of rbIFNτ was estimated to be 23 kDa by Western blotting analysis. We investigated the biological effect of rbIFNτ on prostaglandin (PG) p synthesis in cultured bovine endometrial epithelial cells in the presence or absence of oxytocin (OT, 100 nM). rbIFNτ suppressed basal and OT-induced PGF production in a dose-dependent manner (1-1,000 ng/ml). These results showed that biologically active rbIFNτ was produced in the baculovirus expression system, and that rbIFNτ had the ability to suppress the synthesis of PGF from bovine endometrial epithelial cells.

Original languageEnglish
Pages (from-to)433-440
Number of pages8
JournalJournal of Reproduction and Development
Volume49
Issue number6
DOIs
Publication statusPublished - Dec 1 2003

Keywords

  • Cow
  • Endometrial epithelial cells
  • Interferon (IFN) τ
  • PGF2α

ASJC Scopus subject areas

  • Animal Science and Zoology

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    Takahashi, H., Inumaru, S., Takahashi, M., Watanabe, S., Iga, K., Yokomizo, Y., Geshi, M., Okano, A., & Okuda, K. (2003). Biological activity of recombinant bovine interferon τ using an Autographa californica nuclear polyhedrosis virus expression system. Journal of Reproduction and Development, 49(6), 433-440. https://doi.org/10.1262/jrd.49.433