Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system

Hitomi Takahashi; Makoto Tsunazaki; Takashi Hamano; Masashi Takahashi; Kiyoshi Okuda; Shigeki Inumaru; Akira Okano; Masaya Geshi; Makoto Hirako

doi:10.1292/jvms.12-0403

Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system

Hitomi Takahashi, Makoto Tsunazaki, Takashi Hamano, Masashi Takahashi, Kiyoshi Okuda, Shigeki Inumaru, Akira Okano, Masaya Geshi, Makoto Hirako

Research output: Contribution to journal › Comment/debate › peer-review

2 Citations (Scopus)

Abstract

Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to suppress PGF2α production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same bioactivity as native IFNτ.

Original language	English
Pages (from-to)	447-451
Number of pages	5
Journal	Journal of Veterinary Medical Science
Volume	76
Issue number	3
DOIs	https://doi.org/10.1292/jvms.12-0403
Publication status	Published - 2014
Externally published	Yes

Keywords

Bioactivity
Interferon τ
Maternal-fetal recognition
Recombinant
Silkworm baculovirus

ASJC Scopus subject areas

veterinary(all)

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10.1292/jvms.12-0403

Cite this

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title = "Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system",

abstract = "Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to suppress PGF2α production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same bioactivity as native IFNτ.",

keywords = "Bioactivity, Interferon τ, Maternal-fetal recognition, Recombinant, Silkworm baculovirus",

author = "Hitomi Takahashi and Makoto Tsunazaki and Takashi Hamano and Masashi Takahashi and Kiyoshi Okuda and Shigeki Inumaru and Akira Okano and Masaya Geshi and Makoto Hirako",

year = "2014",

doi = "10.1292/jvms.12-0403",

language = "English",

volume = "76",

pages = "447--451",

journal = "Journal of Veterinary Medical Science",

issn = "0916-7250",

publisher = "Japanese Society of Veterinary Science",

number = "3",

}

TY - JOUR

T1 - Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system

AU - Takahashi, Hitomi

AU - Tsunazaki, Makoto

AU - Hamano, Takashi

AU - Takahashi, Masashi

AU - Okuda, Kiyoshi

AU - Inumaru, Shigeki

AU - Okano, Akira

AU - Geshi, Masaya

AU - Hirako, Makoto

PY - 2014

Y1 - 2014

N2 - Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to suppress PGF2α production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same bioactivity as native IFNτ.

AB - Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to suppress PGF2α production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same bioactivity as native IFNτ.

KW - Bioactivity

KW - Interferon τ

KW - Maternal-fetal recognition

KW - Recombinant

KW - Silkworm baculovirus

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U2 - 10.1292/jvms.12-0403

DO - 10.1292/jvms.12-0403

M3 - Comment/debate

C2 - 24212505

AN - SCOPUS:84897535398

SN - 0916-7250

VL - 76

SP - 447

EP - 451

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

IS - 3

ER -

Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system

Abstract

Keywords

ASJC Scopus subject areas

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