Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system

Hitomi Takahashi, Makoto Tsunazaki, Takashi Hamano, Masashi Takahashi, Kiyoshi Okuda, Shigeki Inumaru, Akira Okano, Masaya Geshi, Makoto Hirako

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vitro development of bovine embryos. Bm-rbIFNτ and OT were shown to suppress PGF2α production in a dose-dependent manner. When in vitro produced morula stage embryos were cultured for 72 hr in modified CR1aa medium supplemented with or without rbIFNτ, Bm-rbIFNτ (10 ng/ml) significantly promoted development to the expanded blastocyst stage. In conclusion, Bm-rbIFNτ was suggested to have the same bioactivity as native IFNτ.

Original languageEnglish
Pages (from-to)447-451
Number of pages5
JournalJournal of Veterinary Medical Science
Volume76
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Bombyx
Baculoviridae
interferons
Bombyx mori
silkworms
Interferons
bioactive properties
Gene Expression
gene expression
cattle
embryo (animal)
Dinoprost
Bombyx mori nucleopolyhedrovirus
oxytocin
morula
blastocyst
prostaglandins
Nucleopolyhedrovirus
Morula
epithelial cells

Keywords

  • Bioactivity
  • Interferon τ
  • Maternal-fetal recognition
  • Recombinant
  • Silkworm baculovirus

ASJC Scopus subject areas

  • veterinary(all)
  • Medicine(all)

Cite this

Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system. / Takahashi, Hitomi; Tsunazaki, Makoto; Hamano, Takashi; Takahashi, Masashi; Okuda, Kiyoshi; Inumaru, Shigeki; Okano, Akira; Geshi, Masaya; Hirako, Makoto.

In: Journal of Veterinary Medical Science, Vol. 76, No. 3, 2014, p. 447-451.

Research output: Contribution to journalArticle

Takahashi, H, Tsunazaki, M, Hamano, T, Takahashi, M, Okuda, K, Inumaru, S, Okano, A, Geshi, M & Hirako, M 2014, 'Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system', Journal of Veterinary Medical Science, vol. 76, no. 3, pp. 447-451. https://doi.org/10.1292/jvms.12-0403
Takahashi, Hitomi ; Tsunazaki, Makoto ; Hamano, Takashi ; Takahashi, Masashi ; Okuda, Kiyoshi ; Inumaru, Shigeki ; Okano, Akira ; Geshi, Masaya ; Hirako, Makoto. / Biological activity of recombinant bovine interferon τ produced by a silkworm- baculovirus gene expression system. In: Journal of Veterinary Medical Science. 2014 ; Vol. 76, No. 3. pp. 447-451.
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