Abstract
Biogenic amines are physiologically neuroactive substances that affect behavioural and physiological traits in invertebrates. In the present study, the effects of dopamine, octopamine, tyramine and serotonin on tonic immobility, or death-feigning, were investigated in Tribolium castaneum. These amines were injected into the abdomens of beetles artificially selected for long or short duration of tonic immobility. In beetles of the long strains, the durations of tonic immobility were shortened by injection of dopamine, octopamine and tyramine, and the effects of these amines were dose-dependent. On the other hand, serotonin injection did not affect the duration of tonic immobility. In the short-strain beetles that rarely feign death, no significant effects of the amines were found on the duration of tonic immobility. Brain expression levels of octopamine, tyramine and serotonin did not differ between long- and short-strain beetles, in contrast to the higher dopamine levels in short strains previously reported. Caffeine decreased the duration of death-feigning in both oral absorption and injection experiments. It is known that caffeine activates dopamine. Therefore, the present results suggest that the duration of tonic immobility is affected by dopamine via the dopamine receptor in T. castaneum.
| Original language | English |
|---|---|
| Pages (from-to) | 622-628 |
| Number of pages | 7 |
| Journal | Journal of Insect Physiology |
| Volume | 56 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2010 |
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Keywords
- Caffeine
- Death-feigning
- Dopamine
- Red flour beetle
- Thanatosis
ASJC Scopus subject areas
- Insect Science
- Physiology
Cite this
Biogenic amines, caffeine and tonic immobility in Tribolium castaneum. / Nishi, Yusuke; Sasaki, Ken; Miyatake, Takahisa.
In: Journal of Insect Physiology, Vol. 56, No. 6, 06.2010, p. 622-628.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biogenic amines, caffeine and tonic immobility in Tribolium castaneum
AU - Nishi, Yusuke
AU - Sasaki, Ken
AU - Miyatake, Takahisa
PY - 2010/6
Y1 - 2010/6
N2 - Biogenic amines are physiologically neuroactive substances that affect behavioural and physiological traits in invertebrates. In the present study, the effects of dopamine, octopamine, tyramine and serotonin on tonic immobility, or death-feigning, were investigated in Tribolium castaneum. These amines were injected into the abdomens of beetles artificially selected for long or short duration of tonic immobility. In beetles of the long strains, the durations of tonic immobility were shortened by injection of dopamine, octopamine and tyramine, and the effects of these amines were dose-dependent. On the other hand, serotonin injection did not affect the duration of tonic immobility. In the short-strain beetles that rarely feign death, no significant effects of the amines were found on the duration of tonic immobility. Brain expression levels of octopamine, tyramine and serotonin did not differ between long- and short-strain beetles, in contrast to the higher dopamine levels in short strains previously reported. Caffeine decreased the duration of death-feigning in both oral absorption and injection experiments. It is known that caffeine activates dopamine. Therefore, the present results suggest that the duration of tonic immobility is affected by dopamine via the dopamine receptor in T. castaneum.
AB - Biogenic amines are physiologically neuroactive substances that affect behavioural and physiological traits in invertebrates. In the present study, the effects of dopamine, octopamine, tyramine and serotonin on tonic immobility, or death-feigning, were investigated in Tribolium castaneum. These amines were injected into the abdomens of beetles artificially selected for long or short duration of tonic immobility. In beetles of the long strains, the durations of tonic immobility were shortened by injection of dopamine, octopamine and tyramine, and the effects of these amines were dose-dependent. On the other hand, serotonin injection did not affect the duration of tonic immobility. In the short-strain beetles that rarely feign death, no significant effects of the amines were found on the duration of tonic immobility. Brain expression levels of octopamine, tyramine and serotonin did not differ between long- and short-strain beetles, in contrast to the higher dopamine levels in short strains previously reported. Caffeine decreased the duration of death-feigning in both oral absorption and injection experiments. It is known that caffeine activates dopamine. Therefore, the present results suggest that the duration of tonic immobility is affected by dopamine via the dopamine receptor in T. castaneum.
KW - Caffeine
KW - Death-feigning
KW - Dopamine
KW - Red flour beetle
KW - Thanatosis
UR - http://www.scopus.com/inward/record.url?scp=77954861376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954861376&partnerID=8YFLogxK
U2 - 10.1016/j.jinsphys.2010.01.002
DO - 10.1016/j.jinsphys.2010.01.002
M3 - Article
VL - 56
SP - 622
EP - 628
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 6
ER -