Biogenic amines, caffeine and tonic immobility in Tribolium castaneum

Yusuke Nishi, Ken Sasaki, Takahisa Miyatake

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Biogenic amines are physiologically neuroactive substances that affect behavioural and physiological traits in invertebrates. In the present study, the effects of dopamine, octopamine, tyramine and serotonin on tonic immobility, or death-feigning, were investigated in Tribolium castaneum. These amines were injected into the abdomens of beetles artificially selected for long or short duration of tonic immobility. In beetles of the long strains, the durations of tonic immobility were shortened by injection of dopamine, octopamine and tyramine, and the effects of these amines were dose-dependent. On the other hand, serotonin injection did not affect the duration of tonic immobility. In the short-strain beetles that rarely feign death, no significant effects of the amines were found on the duration of tonic immobility. Brain expression levels of octopamine, tyramine and serotonin did not differ between long- and short-strain beetles, in contrast to the higher dopamine levels in short strains previously reported. Caffeine decreased the duration of death-feigning in both oral absorption and injection experiments. It is known that caffeine activates dopamine. Therefore, the present results suggest that the duration of tonic immobility is affected by dopamine via the dopamine receptor in T. castaneum.

Original languageEnglish
Pages (from-to)622-628
Number of pages7
JournalJournal of Insect Physiology
Volume56
Issue number6
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Tribolium
Biogenic Amines
Tribolium castaneum
biogenic amines
caffeine
Caffeine
Beetles
Octopamine
Dopamine
dopamine
Tyramine
Tonic Immobility Response
octopamine
duration
tyramine
Amines
Serotonin
amines
serotonin
Coleoptera

Keywords

  • Caffeine
  • Death-feigning
  • Dopamine
  • Red flour beetle
  • Thanatosis

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Biogenic amines, caffeine and tonic immobility in Tribolium castaneum. / Nishi, Yusuke; Sasaki, Ken; Miyatake, Takahisa.

In: Journal of Insect Physiology, Vol. 56, No. 6, 06.2010, p. 622-628.

Research output: Contribution to journalArticle

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