Abstract
Aims: To investigate the effects of inoculating Lactobacillus casei or Lacobacillus buchneri on the production of biogenic amines (BA) in silage. Methods and Results: Wilted festulolium (Lolium perenne × Festuca pratensis), whole crop maize or a total mixed ration, consisting of wet brewer grains, lucerne hay, cracked maize, sugarbeet pulp, soyabean meal and molasses, was ensiled with or without the inoculation of either L. casei (>10 6 CFU g-1) or L. buchneri (>106 CFU g -1). Silages were opened after 60 days of storage, and the concentrations of histamine, tyramine, putrescine and cadaverine were determined. The inoculation of L. casei decreased all the BA regardless of the silage type. The effects of L. buchneri varied between the three silages; the tyramine and putrescine were increased in maize but were lowered in festulolium. Histamine was reduced in festulolium and the by-products, whereas no change was found in the maize silage. None of the inoculant strains produced the four BA in a synthetic medium, accounting for the actual ensiling except for tyramine and putrescine in maize. Conclusions: Wide variation would be found in the production of BA owing to the ensiling materials. The inoculation of L. casei can lower the BA concentration, while the effects of L. buchneri may vary considerably. The screening of BA-producing activity may help to reduce the risk of BA contamination in inoculated silage. Significance and Impact of the Study: Strains of decarboxylase-negative L. buchneri can enhance the aerobic stability of silage without a concern regarding the large production of putrefactive BA.
| Original language | English |
|---|---|
| Pages (from-to) | 325-332 |
| Number of pages | 8 |
| Journal | Journal of Applied Microbiology |
| Volume | 103 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2007 |
Fingerprint
Keywords
- Biogenic amines
- By-products
- Grass
- Lactic acid bacteria
- Maize
- Silage
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Applied Microbiology and Biotechnology
- Biotechnology
- Microbiology
Cite this
Biogenic amine production in grass, maize and total mixed ration silages inoculated with Lactobacillus casei or Lactobacillus buchneri. / Nishino, Naoki; Hattori, H.; Wada, H.; Touno, E.
In: Journal of Applied Microbiology, Vol. 103, No. 2, 08.2007, p. 325-332.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biogenic amine production in grass, maize and total mixed ration silages inoculated with Lactobacillus casei or Lactobacillus buchneri
AU - Nishino, Naoki
AU - Hattori, H.
AU - Wada, H.
AU - Touno, E.
PY - 2007/8
Y1 - 2007/8
N2 - Aims: To investigate the effects of inoculating Lactobacillus casei or Lacobacillus buchneri on the production of biogenic amines (BA) in silage. Methods and Results: Wilted festulolium (Lolium perenne × Festuca pratensis), whole crop maize or a total mixed ration, consisting of wet brewer grains, lucerne hay, cracked maize, sugarbeet pulp, soyabean meal and molasses, was ensiled with or without the inoculation of either L. casei (>10 6 CFU g-1) or L. buchneri (>106 CFU g -1). Silages were opened after 60 days of storage, and the concentrations of histamine, tyramine, putrescine and cadaverine were determined. The inoculation of L. casei decreased all the BA regardless of the silage type. The effects of L. buchneri varied between the three silages; the tyramine and putrescine were increased in maize but were lowered in festulolium. Histamine was reduced in festulolium and the by-products, whereas no change was found in the maize silage. None of the inoculant strains produced the four BA in a synthetic medium, accounting for the actual ensiling except for tyramine and putrescine in maize. Conclusions: Wide variation would be found in the production of BA owing to the ensiling materials. The inoculation of L. casei can lower the BA concentration, while the effects of L. buchneri may vary considerably. The screening of BA-producing activity may help to reduce the risk of BA contamination in inoculated silage. Significance and Impact of the Study: Strains of decarboxylase-negative L. buchneri can enhance the aerobic stability of silage without a concern regarding the large production of putrefactive BA.
AB - Aims: To investigate the effects of inoculating Lactobacillus casei or Lacobacillus buchneri on the production of biogenic amines (BA) in silage. Methods and Results: Wilted festulolium (Lolium perenne × Festuca pratensis), whole crop maize or a total mixed ration, consisting of wet brewer grains, lucerne hay, cracked maize, sugarbeet pulp, soyabean meal and molasses, was ensiled with or without the inoculation of either L. casei (>10 6 CFU g-1) or L. buchneri (>106 CFU g -1). Silages were opened after 60 days of storage, and the concentrations of histamine, tyramine, putrescine and cadaverine were determined. The inoculation of L. casei decreased all the BA regardless of the silage type. The effects of L. buchneri varied between the three silages; the tyramine and putrescine were increased in maize but were lowered in festulolium. Histamine was reduced in festulolium and the by-products, whereas no change was found in the maize silage. None of the inoculant strains produced the four BA in a synthetic medium, accounting for the actual ensiling except for tyramine and putrescine in maize. Conclusions: Wide variation would be found in the production of BA owing to the ensiling materials. The inoculation of L. casei can lower the BA concentration, while the effects of L. buchneri may vary considerably. The screening of BA-producing activity may help to reduce the risk of BA contamination in inoculated silage. Significance and Impact of the Study: Strains of decarboxylase-negative L. buchneri can enhance the aerobic stability of silage without a concern regarding the large production of putrefactive BA.
KW - Biogenic amines
KW - By-products
KW - Grass
KW - Lactic acid bacteria
KW - Maize
KW - Silage
UR - http://www.scopus.com/inward/record.url?scp=34447509919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34447509919&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2672.2006.03244.x
DO - 10.1111/j.1365-2672.2006.03244.x
M3 - Article
C2 - 17650192
AN - SCOPUS:34447509919
VL - 103
SP - 325
EP - 332
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
SN - 1364-5072
IS - 2
ER -