Biofilm Formation by a Fimbriae-Deficient Mutant of Actinobacillus actinomycetemcomitans

Tetsuyoshi Inoue, Ryuji Shingaki, Norio Sogawa, Chiharu Aoki Sogawa, Jun Ichi Asaumi, Susumu Kokeguchi, Kazuhiro Fukui

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Actinobacillus actinomycetemcomitans strain 310-TR produces fimbriae and forms a tight biofilm in broth cultures, without turbid growth. The fimbriae-deficient mutant 310-DF, constructed in this study, was grown as a relatively fragile biofilm at the bottom of a culture vessel. Scanning electron microscopy revealed that on glass coverslips, 310-TR formed tight and spherical microcolonies, while 310-DF produced looser ones. These findings suggest that fimbriae are not essential for the surface-adherent growth but are required for enhancing cell-to-surface and cell-to-cell interactions to stabilize the biofilm. Treatment of the 310-DF biofilm with either sodium metaperiodate or DNase resulted in significant desorption of cells from glass surfaces, indicating that both carbohydrate residues and DNA molecules present on the cell surface are also involved in the biofilm formation.

Original languageEnglish
Pages (from-to)877-881
Number of pages5
JournalMICROBIOLOGY and IMMUNOLOGY
Volume47
Issue number11
DOIs
Publication statusPublished - 2003

Keywords

  • Adherence
  • Biofilm
  • Fimbriae
  • Microcolony formation

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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