Abstract
A dichloromethane (DCM)-degrading bacterium, Ralstonia metallidurans PD11 NBRC 101272, was immobilized in a polyvinyl alcohol (PVA) gel to use in a bioreactor for DCM treatment. After 4-month incubation of PVA gel beads with R. metallidurans PD11 and DCM in a mineral salt medium, the cells were tightly packed in the mesh of the gel. Forty beads of the gel in 10 ml of a batch system model showed effective activity degrading 500 and 1,000 mg l-1 DCM within 2 and 3 h, respectively. Although reduction of pH due to accumulation of chloride ion liberated from DCM decreased the activity, it was recovered by adjustment to neutral pH. The activity of the immobilized cells was not affected by addition of nutrients which were preferentially utilized by R. metallidurans PD11, unlike the activity of the free-living cells. A continuous flow system with a column was more effective for rapid degradation of DCM. Thus, the PVA gel-immobilized cell of R. metallidurans PD11 is thought to be a prospective candidate to develop the bioreactor.
| Original language | English |
|---|---|
| Pages (from-to) | 625-630 |
| Number of pages | 6 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 70 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2006 |
Fingerprint
ASJC Scopus subject areas
- Biotechnology
- Microbiology
- Bioengineering
- Microbiology (medical)
Cite this
Biodegradation of dichloromethane by the polyvinyl alcohol-immobilized methylotrophic bacterium Ralstonia metallidurans PD11. / Miyake-Nakayama, Chizuko; Ikatsu, Hisayoshi; Kashihara, Minoru; Tanaka, Masako; Arita, Michiko; Miyoshi, Shin-ichi; Shinoda, Sumio.
In: Applied Microbiology and Biotechnology, Vol. 70, No. 5, 05.2006, p. 625-630.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biodegradation of dichloromethane by the polyvinyl alcohol-immobilized methylotrophic bacterium Ralstonia metallidurans PD11
AU - Miyake-Nakayama, Chizuko
AU - Ikatsu, Hisayoshi
AU - Kashihara, Minoru
AU - Tanaka, Masako
AU - Arita, Michiko
AU - Miyoshi, Shin-ichi
AU - Shinoda, Sumio
PY - 2006/5
Y1 - 2006/5
N2 - A dichloromethane (DCM)-degrading bacterium, Ralstonia metallidurans PD11 NBRC 101272, was immobilized in a polyvinyl alcohol (PVA) gel to use in a bioreactor for DCM treatment. After 4-month incubation of PVA gel beads with R. metallidurans PD11 and DCM in a mineral salt medium, the cells were tightly packed in the mesh of the gel. Forty beads of the gel in 10 ml of a batch system model showed effective activity degrading 500 and 1,000 mg l-1 DCM within 2 and 3 h, respectively. Although reduction of pH due to accumulation of chloride ion liberated from DCM decreased the activity, it was recovered by adjustment to neutral pH. The activity of the immobilized cells was not affected by addition of nutrients which were preferentially utilized by R. metallidurans PD11, unlike the activity of the free-living cells. A continuous flow system with a column was more effective for rapid degradation of DCM. Thus, the PVA gel-immobilized cell of R. metallidurans PD11 is thought to be a prospective candidate to develop the bioreactor.
AB - A dichloromethane (DCM)-degrading bacterium, Ralstonia metallidurans PD11 NBRC 101272, was immobilized in a polyvinyl alcohol (PVA) gel to use in a bioreactor for DCM treatment. After 4-month incubation of PVA gel beads with R. metallidurans PD11 and DCM in a mineral salt medium, the cells were tightly packed in the mesh of the gel. Forty beads of the gel in 10 ml of a batch system model showed effective activity degrading 500 and 1,000 mg l-1 DCM within 2 and 3 h, respectively. Although reduction of pH due to accumulation of chloride ion liberated from DCM decreased the activity, it was recovered by adjustment to neutral pH. The activity of the immobilized cells was not affected by addition of nutrients which were preferentially utilized by R. metallidurans PD11, unlike the activity of the free-living cells. A continuous flow system with a column was more effective for rapid degradation of DCM. Thus, the PVA gel-immobilized cell of R. metallidurans PD11 is thought to be a prospective candidate to develop the bioreactor.
UR - http://www.scopus.com/inward/record.url?scp=33645715636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645715636&partnerID=8YFLogxK
U2 - 10.1007/s00253-005-0194-4
DO - 10.1007/s00253-005-0194-4
M3 - Article
C2 - 16249875
AN - SCOPUS:33645715636
VL - 70
SP - 625
EP - 630
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -