Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties

Yusuke Shimizu, Yusuke Kawanobe, Toshiisa Konishi, Nobuyuki Kanzawa, Michiyo Honda, Mamoru Aizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have previously synthesized silver-containing hydroxyapatite (Ag-HAp) powders by an ultrasonic spray-pyrolysis (USSP) technique. On the other hand, we have successfully fabricated novel calcium-phosphate cements (CPCs) composed of mainly |3-tricalcium phosphate (|3-TCP) phase with anti-washout property (hereafter, |3-TCP cement), which was set on the basis of chelate-bonding ability of inositol phosphate (IP6). In this study, we developed novel CPCs with both anti-bacterial and anti-washout properties by adding the Ag-HAp powder into the above |3-TCP cements, and examined their anti-bacterial property and cytotoxicity. The Ag-HAp powders with Ag contents of 0, 2, and 5 mol% as a nominal composition were synthesized by an USSP technique. The raw powder for β-TCP cement was prepared by ball-milling the commercially-available |3-TCP powder in the IP6 solution. The Ag-HAp/β-TCP powders were prepared by mixing Ag-HAp powder and β-TCP cement powder at a ratio of 25:75 in mass. The Ag-HAp/|3-TCP cement was fabricated by mixing the above-mentioned Ag-HAp/β-TCP powder and 2.5 mass% Na2HP04solution at a powder/liquid ratio of 1/0.3 [g/cm3 ]. The anti-bacterial property of resulting cements was evaluated using Staphylococcus aureus by biofilm formation test. The Ag-HAp/β-TCP cements containing 2 and 5 mol% Ag showed strong anti-bacterial property among examined specimens. Furthermore, the cytotoxicity of Ag+ ion eluted from these cements was also examined using osteoblastic MC3T3-E1 cells and Transwell® kit. The relative cell viability cultured on each Ag-containing cement specimen was over 80 %, compared with the control (polystyrene plate). These results demonstrate that the present Ag-HAp/β-TCP cements containing 2 mol% Ag are promising one of the candidates as CPCs with both anti-bacterial property and biocompatibility.

Original languageEnglish
Title of host publicationBioceramics 26
PublisherTrans Tech Publications Ltd
Pages107-112
Number of pages6
Volume631
ISBN (Print)9783038352822
DOIs
Publication statusPublished - 2015
Event26th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2014 - Barcelona, Spain
Duration: Nov 6 2014Nov 8 2014

Publication series

NameKey Engineering Materials
Volume631
ISSN (Print)10139826

Other

Other26th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2014
CountrySpain
CityBarcelona
Period11/6/1411/8/14

Fingerprint

Calcium phosphate
Biocompatibility
Silver
Cements
Powders
Spray pyrolysis
Cytotoxicity
calcium phosphate
Phosphates
Ultrasonics
Inositol Phosphates
Polystyrenes
Ball milling
Biofilms
Durapatite
Hydroxyapatite
Cells

Keywords

  • Anti-bacterial property
  • Calcium-phosphate cement
  • Chelate-setting cement
  • Cytotoxicity
  • Inositol phosphate
  • Silver-containing hydroxyapatite

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Shimizu, Y., Kawanobe, Y., Konishi, T., Kanzawa, N., Honda, M., & Aizawa, M. (2015). Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties. In Bioceramics 26 (Vol. 631, pp. 107-112). (Key Engineering Materials; Vol. 631). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net.KEM.631.107

Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties. / Shimizu, Yusuke; Kawanobe, Yusuke; Konishi, Toshiisa; Kanzawa, Nobuyuki; Honda, Michiyo; Aizawa, Mamoru.

Bioceramics 26. Vol. 631 Trans Tech Publications Ltd, 2015. p. 107-112 (Key Engineering Materials; Vol. 631).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shimizu, Y, Kawanobe, Y, Konishi, T, Kanzawa, N, Honda, M & Aizawa, M 2015, Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties. in Bioceramics 26. vol. 631, Key Engineering Materials, vol. 631, Trans Tech Publications Ltd, pp. 107-112, 26th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2014, Barcelona, Spain, 11/6/14. https://doi.org/10.4028/www.scientific.net.KEM.631.107
Shimizu Y, Kawanobe Y, Konishi T, Kanzawa N, Honda M, Aizawa M. Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties. In Bioceramics 26. Vol. 631. Trans Tech Publications Ltd. 2015. p. 107-112. (Key Engineering Materials). https://doi.org/10.4028/www.scientific.net.KEM.631.107
Shimizu, Yusuke ; Kawanobe, Yusuke ; Konishi, Toshiisa ; Kanzawa, Nobuyuki ; Honda, Michiyo ; Aizawa, Mamoru. / Biocompatibility of silver-Containing calcium-Phosphate cements with anti-Bacterial properties. Bioceramics 26. Vol. 631 Trans Tech Publications Ltd, 2015. pp. 107-112 (Key Engineering Materials).
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abstract = "We have previously synthesized silver-containing hydroxyapatite (Ag-HAp) powders by an ultrasonic spray-pyrolysis (USSP) technique. On the other hand, we have successfully fabricated novel calcium-phosphate cements (CPCs) composed of mainly |3-tricalcium phosphate (|3-TCP) phase with anti-washout property (hereafter, |3-TCP cement), which was set on the basis of chelate-bonding ability of inositol phosphate (IP6). In this study, we developed novel CPCs with both anti-bacterial and anti-washout properties by adding the Ag-HAp powder into the above |3-TCP cements, and examined their anti-bacterial property and cytotoxicity. The Ag-HAp powders with Ag contents of 0, 2, and 5 mol{\%} as a nominal composition were synthesized by an USSP technique. The raw powder for β-TCP cement was prepared by ball-milling the commercially-available |3-TCP powder in the IP6 solution. The Ag-HAp/β-TCP powders were prepared by mixing Ag-HAp powder and β-TCP cement powder at a ratio of 25:75 in mass. The Ag-HAp/|3-TCP cement was fabricated by mixing the above-mentioned Ag-HAp/β-TCP powder and 2.5 mass{\%} Na2HP04solution at a powder/liquid ratio of 1/0.3 [g/cm3 ]. The anti-bacterial property of resulting cements was evaluated using Staphylococcus aureus by biofilm formation test. The Ag-HAp/β-TCP cements containing 2 and 5 mol{\%} Ag showed strong anti-bacterial property among examined specimens. Furthermore, the cytotoxicity of Ag+ ion eluted from these cements was also examined using osteoblastic MC3T3-E1 cells and Transwell{\circledR} kit. The relative cell viability cultured on each Ag-containing cement specimen was over 80 {\%}, compared with the control (polystyrene plate). These results demonstrate that the present Ag-HAp/β-TCP cements containing 2 mol{\%} Ag are promising one of the candidates as CPCs with both anti-bacterial property and biocompatibility.",
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N2 - We have previously synthesized silver-containing hydroxyapatite (Ag-HAp) powders by an ultrasonic spray-pyrolysis (USSP) technique. On the other hand, we have successfully fabricated novel calcium-phosphate cements (CPCs) composed of mainly |3-tricalcium phosphate (|3-TCP) phase with anti-washout property (hereafter, |3-TCP cement), which was set on the basis of chelate-bonding ability of inositol phosphate (IP6). In this study, we developed novel CPCs with both anti-bacterial and anti-washout properties by adding the Ag-HAp powder into the above |3-TCP cements, and examined their anti-bacterial property and cytotoxicity. The Ag-HAp powders with Ag contents of 0, 2, and 5 mol% as a nominal composition were synthesized by an USSP technique. The raw powder for β-TCP cement was prepared by ball-milling the commercially-available |3-TCP powder in the IP6 solution. The Ag-HAp/β-TCP powders were prepared by mixing Ag-HAp powder and β-TCP cement powder at a ratio of 25:75 in mass. The Ag-HAp/|3-TCP cement was fabricated by mixing the above-mentioned Ag-HAp/β-TCP powder and 2.5 mass% Na2HP04solution at a powder/liquid ratio of 1/0.3 [g/cm3 ]. The anti-bacterial property of resulting cements was evaluated using Staphylococcus aureus by biofilm formation test. The Ag-HAp/β-TCP cements containing 2 and 5 mol% Ag showed strong anti-bacterial property among examined specimens. Furthermore, the cytotoxicity of Ag+ ion eluted from these cements was also examined using osteoblastic MC3T3-E1 cells and Transwell® kit. The relative cell viability cultured on each Ag-containing cement specimen was over 80 %, compared with the control (polystyrene plate). These results demonstrate that the present Ag-HAp/β-TCP cements containing 2 mol% Ag are promising one of the candidates as CPCs with both anti-bacterial property and biocompatibility.

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KW - Cytotoxicity

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KW - Silver-containing hydroxyapatite

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