Biochemical properties of the major outer membrane proteins of Porphyromonas gingivalis.

Susumu Kokeguchi, M. Miyamoto, H. Ohyama, H. Hongyo, Masaharu Takigawa, H. Kurihara, Y. Murayama, K. Kato

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Abstract

The major outer membrane proteins (MOMP) of 53 and 67 kDa were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in outer membrane enriched fractions of Porphyromonas gingivalis FDC 381 and ATCC 33277, respectively. The MOMP were purified by anion exchange and gel filtration chromatography after extraction with Zwittergent 3-14. Their reactivity to monoclonal antibodies and their amino acid composition were analysed. The N-terminal amino acid sequences of twenty residues of both purified MOMP were determined and compared with those previously reported for MOMP of other periodontopathic bacteria.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalMicrobios
Volume77
Issue number313
Publication statusPublished - 1994

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ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Kokeguchi, S., Miyamoto, M., Ohyama, H., Hongyo, H., Takigawa, M., Kurihara, H., Murayama, Y., & Kato, K. (1994). Biochemical properties of the major outer membrane proteins of Porphyromonas gingivalis. Microbios, 77(313), 247-252.