Biochemical properties of the major outer membrane proteins of Porphyromonas gingivalis.

Susumu Kokeguchi, M. Miyamoto, H. Ohyama, H. Hongyo, Masaharu Takigawa, H. Kurihara, Y. Murayama, K. Kato

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The major outer membrane proteins (MOMP) of 53 and 67 kDa were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in outer membrane enriched fractions of Porphyromonas gingivalis FDC 381 and ATCC 33277, respectively. The MOMP were purified by anion exchange and gel filtration chromatography after extraction with Zwittergent 3-14. Their reactivity to monoclonal antibodies and their amino acid composition were analysed. The N-terminal amino acid sequences of twenty residues of both purified MOMP were determined and compared with those previously reported for MOMP of other periodontopathic bacteria.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalMicrobios
Volume77
Issue number313
Publication statusPublished - 1994

Fingerprint

Porphyromonas gingivalis
Membrane Proteins
zwittergent 3-14
Sodium Dodecyl Sulfate
Gel Chromatography
Anions
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Monoclonal Antibodies
Bacteria
Amino Acids
Membranes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Kokeguchi, S., Miyamoto, M., Ohyama, H., Hongyo, H., Takigawa, M., Kurihara, H., ... Kato, K. (1994). Biochemical properties of the major outer membrane proteins of Porphyromonas gingivalis. Microbios, 77(313), 247-252.

Biochemical properties of the major outer membrane proteins of Porphyromonas gingivalis. / Kokeguchi, Susumu; Miyamoto, M.; Ohyama, H.; Hongyo, H.; Takigawa, Masaharu; Kurihara, H.; Murayama, Y.; Kato, K.

In: Microbios, Vol. 77, No. 313, 1994, p. 247-252.

Research output: Contribution to journalArticle

Kokeguchi, S, Miyamoto, M, Ohyama, H, Hongyo, H, Takigawa, M, Kurihara, H, Murayama, Y & Kato, K 1994, 'Biochemical properties of the major outer membrane proteins of Porphyromonas gingivalis.', Microbios, vol. 77, no. 313, pp. 247-252.
Kokeguchi, Susumu ; Miyamoto, M. ; Ohyama, H. ; Hongyo, H. ; Takigawa, Masaharu ; Kurihara, H. ; Murayama, Y. ; Kato, K. / Biochemical properties of the major outer membrane proteins of Porphyromonas gingivalis. In: Microbios. 1994 ; Vol. 77, No. 313. pp. 247-252.
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