Biochemical characterization of photosystem I-associated light-harvesting complexes i and II isolated from state 2 cells of chlamydomonas reinhardtii

Hiroko Takahashi, Akira Okamuro, Jun Minagawa, Yuichiro Takahashi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Two photosystems, PSI and PSII, drive electron transfer in series for oxygenic photosynthesis using light energy. To balance the activity of the two photosystems under varying light conditions, mobile antenna complexes, light-harvesting complex IIs (LHCIIs), shuttle between the two photosystems during state transitions. PSI forms a complex consisting of PSI core and its peripheral light-harvesting complex (LHCI) in plants and algae. In a previous study, we isolated a PSI-LHCI-LHCII supercomplex containing both LHCI and LHCII from state 2 cells of Chlamydomonas reinhardtii. In the present study, we isolated a PSI-LHCI-LHCII supercomplex associating with more LHCII complexes under a further optimized protocol. We determined its antenna size by three independent methods and revealed that the associated LHCIIs increased the antenna size by about 70 Chls and transferred light energy to the PSI core. Uniform labeling of total cellular proteins with 14C indicated that the PSI-LHCI-LHCII supercomplex contains 1.85 copies of LhcbM5 and CP29 and 1.29 copies of CP26. PSI-LHCI-LHCII also stably bound 0.4 copy of ferredoxin-NADP+ oxidoreductase (FNR) that catalyzes light-induced electron transfer from PSI to NADP+ in the presence of ferredoxin. We discuss the possible organization of these LHCIIs in the PSI-LHCI-LHCII supercomplex.

Original languageEnglish
Pages (from-to)1437-1449
Number of pages13
JournalPlant and Cell Physiology
Volume55
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Photosystem I Protein Complex
Chlamydomonas reinhardtii
light harvesting complex
photosystem I
Light
cells
antennae
algae
electron transfer
ferredoxin-NADP reductase
Light-Harvesting Protein Complexes
ferredoxins
Ferredoxin-NADP Reductase
Electrons
energy
Ferredoxins
Photosynthesis
NADP

Keywords

  • Antenna complex
  • Isotope labeling
  • Phosphorylation
  • Photosystem I
  • State transitions

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology
  • Medicine(all)

Cite this

Biochemical characterization of photosystem I-associated light-harvesting complexes i and II isolated from state 2 cells of chlamydomonas reinhardtii. / Takahashi, Hiroko; Okamuro, Akira; Minagawa, Jun; Takahashi, Yuichiro.

In: Plant and Cell Physiology, Vol. 55, No. 8, 2014, p. 1437-1449.

Research output: Contribution to journalArticle

@article{f65596d88ce34d43855d5d05543a9a5c,
title = "Biochemical characterization of photosystem I-associated light-harvesting complexes i and II isolated from state 2 cells of chlamydomonas reinhardtii",
abstract = "Two photosystems, PSI and PSII, drive electron transfer in series for oxygenic photosynthesis using light energy. To balance the activity of the two photosystems under varying light conditions, mobile antenna complexes, light-harvesting complex IIs (LHCIIs), shuttle between the two photosystems during state transitions. PSI forms a complex consisting of PSI core and its peripheral light-harvesting complex (LHCI) in plants and algae. In a previous study, we isolated a PSI-LHCI-LHCII supercomplex containing both LHCI and LHCII from state 2 cells of Chlamydomonas reinhardtii. In the present study, we isolated a PSI-LHCI-LHCII supercomplex associating with more LHCII complexes under a further optimized protocol. We determined its antenna size by three independent methods and revealed that the associated LHCIIs increased the antenna size by about 70 Chls and transferred light energy to the PSI core. Uniform labeling of total cellular proteins with 14C indicated that the PSI-LHCI-LHCII supercomplex contains 1.85 copies of LhcbM5 and CP29 and 1.29 copies of CP26. PSI-LHCI-LHCII also stably bound 0.4 copy of ferredoxin-NADP+ oxidoreductase (FNR) that catalyzes light-induced electron transfer from PSI to NADP+ in the presence of ferredoxin. We discuss the possible organization of these LHCIIs in the PSI-LHCI-LHCII supercomplex.",
keywords = "Antenna complex, Isotope labeling, Phosphorylation, Photosystem I, State transitions",
author = "Hiroko Takahashi and Akira Okamuro and Jun Minagawa and Yuichiro Takahashi",
year = "2014",
doi = "10.1093/pcp/pcu071",
language = "English",
volume = "55",
pages = "1437--1449",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "8",

}

TY - JOUR

T1 - Biochemical characterization of photosystem I-associated light-harvesting complexes i and II isolated from state 2 cells of chlamydomonas reinhardtii

AU - Takahashi, Hiroko

AU - Okamuro, Akira

AU - Minagawa, Jun

AU - Takahashi, Yuichiro

PY - 2014

Y1 - 2014

N2 - Two photosystems, PSI and PSII, drive electron transfer in series for oxygenic photosynthesis using light energy. To balance the activity of the two photosystems under varying light conditions, mobile antenna complexes, light-harvesting complex IIs (LHCIIs), shuttle between the two photosystems during state transitions. PSI forms a complex consisting of PSI core and its peripheral light-harvesting complex (LHCI) in plants and algae. In a previous study, we isolated a PSI-LHCI-LHCII supercomplex containing both LHCI and LHCII from state 2 cells of Chlamydomonas reinhardtii. In the present study, we isolated a PSI-LHCI-LHCII supercomplex associating with more LHCII complexes under a further optimized protocol. We determined its antenna size by three independent methods and revealed that the associated LHCIIs increased the antenna size by about 70 Chls and transferred light energy to the PSI core. Uniform labeling of total cellular proteins with 14C indicated that the PSI-LHCI-LHCII supercomplex contains 1.85 copies of LhcbM5 and CP29 and 1.29 copies of CP26. PSI-LHCI-LHCII also stably bound 0.4 copy of ferredoxin-NADP+ oxidoreductase (FNR) that catalyzes light-induced electron transfer from PSI to NADP+ in the presence of ferredoxin. We discuss the possible organization of these LHCIIs in the PSI-LHCI-LHCII supercomplex.

AB - Two photosystems, PSI and PSII, drive electron transfer in series for oxygenic photosynthesis using light energy. To balance the activity of the two photosystems under varying light conditions, mobile antenna complexes, light-harvesting complex IIs (LHCIIs), shuttle between the two photosystems during state transitions. PSI forms a complex consisting of PSI core and its peripheral light-harvesting complex (LHCI) in plants and algae. In a previous study, we isolated a PSI-LHCI-LHCII supercomplex containing both LHCI and LHCII from state 2 cells of Chlamydomonas reinhardtii. In the present study, we isolated a PSI-LHCI-LHCII supercomplex associating with more LHCII complexes under a further optimized protocol. We determined its antenna size by three independent methods and revealed that the associated LHCIIs increased the antenna size by about 70 Chls and transferred light energy to the PSI core. Uniform labeling of total cellular proteins with 14C indicated that the PSI-LHCI-LHCII supercomplex contains 1.85 copies of LhcbM5 and CP29 and 1.29 copies of CP26. PSI-LHCI-LHCII also stably bound 0.4 copy of ferredoxin-NADP+ oxidoreductase (FNR) that catalyzes light-induced electron transfer from PSI to NADP+ in the presence of ferredoxin. We discuss the possible organization of these LHCIIs in the PSI-LHCI-LHCII supercomplex.

KW - Antenna complex

KW - Isotope labeling

KW - Phosphorylation

KW - Photosystem I

KW - State transitions

UR - http://www.scopus.com/inward/record.url?scp=84906050988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906050988&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcu071

DO - 10.1093/pcp/pcu071

M3 - Article

VL - 55

SP - 1437

EP - 1449

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 8

ER -