Biochemical changes in the central nervous system of rats exposed to 1-bromopropane for seven days

Hailan Wang, Gaku Ichihara, Hidenori Ito, Kanefusa Kato, Junzoh Kitoh, Tetsuya Yamada, Xiaozhong Yu, Seiji Tsuboi, Yoshinori Moriyama, Rie Sakatani, Eiji Shibata, Michihiro Kamijima, Seiichiro Itohara, Yasuhiro Takeuchi

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Abstract

1-Bromopropane is used widely as an alternative to ozone-depleting solvents. The neurotoxic effects of this agent have been described in humans and experimental animals. Here we investigated the underlying mechanisms of the neurotoxic effects of 1-bromopropane by examining the initial biochemical changes in the central nervous system. Four groups of 9 Wistar male rats each were exposed to 200, 400, or 800 ppm 1-bromopropane or only fresh air, 8 h per day for 7 days. At the end of the experiment, the cerebrum, cerebellum, brain stem and lumbar enlargement of the spinal cord were dissected out from each rat (n = 8) for biochemical analyses. Morphological examinations of the nervous system were performed in the remaining rat of each group. 1-Bromopropane dose-dependently decreased neurospecific γ-enolase, total glutathione, and nonprotein sulfhydryl groups in the cerebrum and cerebellum. Creatine kinase activity decreased dose-dependently in the brain and spinal cord. Histopathological examination showed swelling of preterminal axons in gracile nucleus and degeneration of myelin in peripheral nerves. Our results of low levels of γ-enolase suggested that 1-bromopropane might primarily cause functional or cellular loss of neurons in the cerebrum and cerebellum. Glutathione depletion or modification to functional proteins containing a sulfhydryl base as a critical site might be the underlying mechanism of 1-bromopropane neurotoxicity.

Original languageEnglish
Pages (from-to)114-120
Number of pages7
JournalToxicological Sciences
Volume67
Issue number1
DOIs
Publication statusPublished - 2002

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Neurology
Rats
Central Nervous System
Cerebrum
Cerebellum
Phosphopyruvate Hydratase
Glutathione
Brain
Spinal Cord
Ozone
Creatine Kinase
Myelin Sheath
Peripheral Nerves
Nervous System
Brain Stem
Neurons
Axons
Swelling
1-bromopropane
Wistar Rats

Keywords

  • γ-enolase
  • 1-bromopropane
  • Creatine kinase
  • Glutathione
  • Neurotoxicity
  • Peripheral nerve

ASJC Scopus subject areas

  • Toxicology

Cite this

Wang, H., Ichihara, G., Ito, H., Kato, K., Kitoh, J., Yamada, T., ... Takeuchi, Y. (2002). Biochemical changes in the central nervous system of rats exposed to 1-bromopropane for seven days. Toxicological Sciences, 67(1), 114-120. https://doi.org/10.1093/toxsci/67.1.114

Biochemical changes in the central nervous system of rats exposed to 1-bromopropane for seven days. / Wang, Hailan; Ichihara, Gaku; Ito, Hidenori; Kato, Kanefusa; Kitoh, Junzoh; Yamada, Tetsuya; Yu, Xiaozhong; Tsuboi, Seiji; Moriyama, Yoshinori; Sakatani, Rie; Shibata, Eiji; Kamijima, Michihiro; Itohara, Seiichiro; Takeuchi, Yasuhiro.

In: Toxicological Sciences, Vol. 67, No. 1, 2002, p. 114-120.

Research output: Contribution to journalArticle

Wang, H, Ichihara, G, Ito, H, Kato, K, Kitoh, J, Yamada, T, Yu, X, Tsuboi, S, Moriyama, Y, Sakatani, R, Shibata, E, Kamijima, M, Itohara, S & Takeuchi, Y 2002, 'Biochemical changes in the central nervous system of rats exposed to 1-bromopropane for seven days', Toxicological Sciences, vol. 67, no. 1, pp. 114-120. https://doi.org/10.1093/toxsci/67.1.114
Wang, Hailan ; Ichihara, Gaku ; Ito, Hidenori ; Kato, Kanefusa ; Kitoh, Junzoh ; Yamada, Tetsuya ; Yu, Xiaozhong ; Tsuboi, Seiji ; Moriyama, Yoshinori ; Sakatani, Rie ; Shibata, Eiji ; Kamijima, Michihiro ; Itohara, Seiichiro ; Takeuchi, Yasuhiro. / Biochemical changes in the central nervous system of rats exposed to 1-bromopropane for seven days. In: Toxicological Sciences. 2002 ; Vol. 67, No. 1. pp. 114-120.
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