Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes

Naoki Wada, Shin'Ichiro Kajiyama, Yukio Akiyama, Shigeki Kawakami, Daisuke No, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Naoko Nose, Go Suzuki, Yasuhiko Mukai, Kiichi Fukui

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Transformation with large DNA molecules enables multiple genes to be introduced into plants simultaneously to produce transgenic plants with complex phenotypes. In this study, a large DNA fragment (ca. 100 kb) containing a set of Aegilops tauschii hardness genes was introduced into rice plants using a novel transformation method, called bioactive beads-mediated transformation. Nine transgenic rice plants were obtained and the presence of transgenes in the rice genome was confirmed by PCR and FISH analyses. The results suggested that multiple transgenes were successfully integrated in all transgenic plants. The expression of one of the transgenes, puroindoline b, was confirmed at the mRNA and protein levels in the T2 generation. Our study clearly demonstrates that the bioactive bead method is capable of producing transgenic rice plants carrying large DNA fragments. This method will facilitate the production of useful transgenic plants by introducing multiple genes simultaneously.

Original languageEnglish
Pages (from-to)759-768
Number of pages10
JournalPlant Cell Reports
Volume28
Issue number5
DOIs
Publication statusPublished - May 2009
Externally publishedYes

Keywords

  • Bioactive beads-mediated transformation
  • Hardness genes
  • Plant transformation
  • Rice
  • Transformation with large DNA fragment

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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