Bio-Nanocapsule-Liposome Conjugates for In Vivo Pinpoint Drug and Gene Delivery

Takeshi Kasuya, Joohee Jung, Rie Kinoshita, Yasumasa Goh, Takashi Matsuzaki, Masumi Iijima, Nobuo Yoshimoto, Katsuyuki Tanizawa, Shun'ichi Kuroda

Research output: Contribution to journalReview articlepeer-review

28 Citations (Scopus)

Abstract

A bio-nanocapsule (BNC) is an ~ 50-nm hepatitis B virus (HBV) subviral particle comprising HBV envelope L proteins and a lipid bilayer, and is synthesized in recombinant Saccharomyces cerevisiae. When BNCs are administered intravenously in a mouse xenograft model, they can accumulate specifically in human liver-derived tissues and enter cells efficiently by the HBV-derived human liver-specific infection machinery, localized at the outer-membrane pre-S region of the L protein. BNC specificity for the human liver can be altered to other tissues by substituting the pre-S region using targeting molecules (e.g., antibodies, lectins, cytokines). BNCs can spontaneously form complexes with liposomes (LPs) by the membrane fusogenic activity of the pre-S region. LPs containing various therapeutic materials (e.g., chemicals, proteins, DNA, RNA) can therefore be covered with BNCs to form an ~ 150-nm BNC-LP conjugate. BNC-LP conjugates injected intravenously can deliver incorporated materials to target tissues specifically and efficiently by utilizing the HBV-derived infection machinery. The stability of BNC-LP conjugates in the blood circulation is similar to that of PEGylated LPs. In this chapter, we describe the preparation and in vivo application of BNC-LP conjugates, and the potential of BNC-LP conjugates as in vivo pinpoint drug delivery systems.

Original languageEnglish
Pages (from-to)147-166
Number of pages20
JournalMethods in Enzymology
Volume464
Issue numberC
DOIs
Publication statusPublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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