Bio-catalyzer α·ρ no. 11 (bio-normalizer) supplementation: Effect on oxidative stress to isolated rat hearts

N. Haramaki, L. Marcocci, R. D'Anna, L. J. Yan, Hirotsugu Kobuchi, L. Packer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bio-Catalyzer α·ρ No. 11 (Bio-Normalizer), a natural health food product prepared by yeast fermentation of medicinal plants, has been recently reported to posses antioxidant properties. To better define its antioxidant action, we investigated the effects of orally supplemented Bio-Normalizer on oxidative damage in the rat heart. Hearts were isolated from control or Bio-Normalizer supplemented animals and 1) exposed to ischemia-reperfusion using the Langendorff technique, or 2) homogenized and exposed to peroxyl radicals generated from (2,2'-azobis (2,4-dimethylvaleronitrile) (AMVN). During reperfusion following 40 minutes of ischemia, leakage of lactate dehydrogenase from hearts isolated from Bio-Normalizer supplemented rats was significantly lower than from hearts of control animals. Furthermore, lower levels of AMVN-induced accumulation of thiobarbituric acid reactive substances and of protein carbonyl derivatives were measured in homogenates prepared from hearts isolated from Bio-Normalizer supplemented rats than in samples from control animals. Our findings confirm an antioxidant action of Bio-Normalizer and show that it protects the heart against ischemia-reperfusion induced damage.

Original languageEnglish
Pages (from-to)1263-1268
Number of pages6
JournalBiochemistry and Molecular Biology International
Volume36
Issue number6
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Oxidative stress
Rats
Oxidative Stress
Animals
Ischemia
Antioxidants
Reperfusion
Thiobarbituric Acid Reactive Substances
Medicinal Plants
Reperfusion Injury
L-Lactate Dehydrogenase
Yeast
Fermentation
Yeasts
Health
Derivatives
Food
2,2'-azobis(2,4-dimethylvaleronitrile)
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Bio-catalyzer α·ρ no. 11 (bio-normalizer) supplementation : Effect on oxidative stress to isolated rat hearts. / Haramaki, N.; Marcocci, L.; D'Anna, R.; Yan, L. J.; Kobuchi, Hirotsugu; Packer, L.

In: Biochemistry and Molecular Biology International, Vol. 36, No. 6, 1995, p. 1263-1268.

Research output: Contribution to journalArticle

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