TY - JOUR
T1 - Binucleation of accessory gland lobe contributes to effective ejection of seminal fluid in drosophila melanogaster
AU - Taniguchi, Kiichiro
AU - Kokuryo, Akihiko
AU - Imano, Takao
AU - Nakagoshi, Hideki
AU - Adachi-Yamada, Takashi
N1 - Funding Information:
We thank Jean-René Huynh for supplying us with aurB2A43 and aurB35.33 fly lines, Toshiro Aigaki for the Sp-GFP fly line, the Bloomington Stock Center and Kyoto Stock Center for other fly stocks, Developmental Studies Hybridoma Bank at the University of Iowa for antibodies, Drosophila Genomics Resource Center for cDNA clones, and FlyC31 for pUASattB plasmid. We also thank Noriyuki Wakamatsu/ Kiyokazu Agata/ Akihiko Takashima for helping us with sandwich ELISA analysis, and Eurofins Genomics for DNA sequencings. This work was supported by JSPS KAKENHI (Grant-in-Aid for Young Scientists (B)) [22770196 to KT], (Grant-in-Aid for Scientific Research on Priority Areas) [20058021 to TAY] and (Grant-in-Aid for Scientific Research (C)) [20570202 to TAY].
Publisher Copyright:
© 2018 Zoological Society of Japan.
PY - 2018/10
Y1 - 2018/10
N2 - The adult male accessory gland in insects is an internal reproductive organ analogous to the mammalian prostate, and secretes various components in the seminal fluid. Products of the accessory gland in the fruit fly Drosophila melanogaster are known to control reproductive behaviors in mated females, such as food uptake, oviposition rate, and rejection of re-mating with other males, all of which increase male reproductive capacity. Production of larger amounts of accessory gland products is thus thought to result in higher male reproductive success. The epithelium of the Drosophila accessory gland lobe is composed of a unique population of binucleate cells. We previously predicted, based on measurements of cell size in mono/binucleate mosaic accessory glands, that binucleation results in a higher plasticity in cell shape, enabling more effective ejection of seminal fluid. However, the actual effect of binucleation on ejection of seminal fluid or reproductive capacity remained unclear, as we were unable to generate an organ with uniformly mononucleate cells. In the present study, we generated organs in which most of the epithelial cells are mononucleate by manipulating aurora B or fizzy-related to block binucleation. Mononucleation resulted in a less elastic accessory gland lobe, which decreased ejection volume and the oviposition of mated females; these effects were particularly pronounced over the long term. These results suggest that binucleation in accessory gland epithelial cells contributes to higher plasticity in the volume of this organ, and enhances male reproductive success through enabling ejection of larger amounts of seminal fluid.
AB - The adult male accessory gland in insects is an internal reproductive organ analogous to the mammalian prostate, and secretes various components in the seminal fluid. Products of the accessory gland in the fruit fly Drosophila melanogaster are known to control reproductive behaviors in mated females, such as food uptake, oviposition rate, and rejection of re-mating with other males, all of which increase male reproductive capacity. Production of larger amounts of accessory gland products is thus thought to result in higher male reproductive success. The epithelium of the Drosophila accessory gland lobe is composed of a unique population of binucleate cells. We previously predicted, based on measurements of cell size in mono/binucleate mosaic accessory glands, that binucleation results in a higher plasticity in cell shape, enabling more effective ejection of seminal fluid. However, the actual effect of binucleation on ejection of seminal fluid or reproductive capacity remained unclear, as we were unable to generate an organ with uniformly mononucleate cells. In the present study, we generated organs in which most of the epithelial cells are mononucleate by manipulating aurora B or fizzy-related to block binucleation. Mononucleation resulted in a less elastic accessory gland lobe, which decreased ejection volume and the oviposition of mated females; these effects were particularly pronounced over the long term. These results suggest that binucleation in accessory gland epithelial cells contributes to higher plasticity in the volume of this organ, and enhances male reproductive success through enabling ejection of larger amounts of seminal fluid.
KW - Drosophila melanogaster
KW - accessory gland protein
KW - binucleate state
KW - male accessory gland
KW - reproduction
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U2 - 10.2108/zs170188
DO - 10.2108/zs170188
M3 - Article
C2 - 30298781
AN - SCOPUS:85054618159
VL - 35
SP - 446
EP - 458
JO - Zoological Science
JF - Zoological Science
SN - 0289-0003
IS - 5
ER -