Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane

Shunji Hitotsubashi, Yoshio Fujii, Keinosuke Okamoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.

Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalFEMS Microbiology Letters
Volume122
Issue number3
DOIs
Publication statusPublished - Oct 1 1994
Externally publishedYes

Keywords

  • Escherichia coli
  • Heat-stable enterotoxin
  • Membrane protein
  • Receptor

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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