Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane

Shunji Hitotsubashi, Yoshio Fujii, Keinosuke Okamoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.

Original languageEnglish
Pages (from-to)297-302
Number of pages6
JournalFEMS Microbiology Letters
Volume122
Issue number3
Publication statusPublished - Oct 1 1994
Externally publishedYes

Fingerprint

Enterotoxins
Carrier Proteins
Escherichia coli
Membranes
Proteins
Durapatite
Protein Binding
Intestines
Gel Chromatography
Chromatography
Gels
Cell Membrane
heat stable toxin (E coli)

Keywords

  • Escherichia coli
  • Heat-stable enterotoxin
  • Membrane protein
  • Receptor

ASJC Scopus subject areas

  • Microbiology
  • Medicine(all)
  • Molecular Biology
  • Genetics

Cite this

Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane. / Hitotsubashi, Shunji; Fujii, Yoshio; Okamoto, Keinosuke.

In: FEMS Microbiology Letters, Vol. 122, No. 3, 01.10.1994, p. 297-302.

Research output: Contribution to journalArticle

@article{08097b27a04e4dfe882eb3db4aa80b33,
title = "Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane",
abstract = "The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.",
keywords = "Escherichia coli, Heat-stable enterotoxin, Membrane protein, Receptor",
author = "Shunji Hitotsubashi and Yoshio Fujii and Keinosuke Okamoto",
year = "1994",
month = "10",
day = "1",
language = "English",
volume = "122",
pages = "297--302",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Binding protein for Escherichia coli heat-stable enterotoxin II in mouse intestinal membrane

AU - Hitotsubashi, Shunji

AU - Fujii, Yoshio

AU - Okamoto, Keinosuke

PY - 1994/10/1

Y1 - 1994/10/1

N2 - The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.

AB - The protein binding Escherichia coli heat-stable enterotoxin II (STII) was isolated from cell membranes of mouse intestine. The binding of 125I-labeled STII to the proteins was inhibited by unlabeled STII, showing that it is specific. Proteins cross-linked with 125I-STII were purified by column chromatography on hydroxyapatite and TSK gel. Analyses of the purified protein by SDS-polyacrylamide gel electrophorosis and gel filtration showed that the molecular mass was 25 kDa.

KW - Escherichia coli

KW - Heat-stable enterotoxin

KW - Membrane protein

KW - Receptor

UR - http://www.scopus.com/inward/record.url?scp=0027930124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027930124&partnerID=8YFLogxK

M3 - Article

C2 - 7988871

AN - SCOPUS:0027930124

VL - 122

SP - 297

EP - 302

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 3

ER -