Abstract
The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequene. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.
| Original language | English |
|---|---|
| Pages (from-to) | 3479-3483 |
| Number of pages | 5 |
| Journal | Journal of Biological Chemistry |
| Volume | 265 |
| Issue number | 6 |
| Publication status | Published - Mar 23 1990 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
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Cite this
Nakagoshi, H., Nagase, T., Kanei-Ishii, C., Ueno, Y., & Ishii, S. (1990). Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription. Journal of Biological Chemistry, 265(6), 3479-3483.