Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription

Hideki Nakagoshi, Takahiro Nagase, Chie Kanei-Ishii, Yoshio Ueno, Shunsuke Ishii

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequence. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.

Original languageEnglish
Pages (from-to)3479-3483
Number of pages5
JournalJournal of Biological Chemistry
Volume265
Issue number6
Publication statusPublished - Feb 25 1990
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins c-myb
Simian virus 40
Transcription
Viruses
myb Genes
Tandem Repeat Sequences
T-cells
Nuclear Proteins
Transfection
Carrier Proteins
Plasmids
T-Lymphocytes
Cell Line
DNA
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription. / Nakagoshi, Hideki; Nagase, Takahiro; Kanei-Ishii, Chie; Ueno, Yoshio; Ishii, Shunsuke.

In: Journal of Biological Chemistry, Vol. 265, No. 6, 25.02.1990, p. 3479-3483.

Research output: Contribution to journalArticle

Nakagoshi, Hideki ; Nagase, Takahiro ; Kanei-Ishii, Chie ; Ueno, Yoshio ; Ishii, Shunsuke. / Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 6. pp. 3479-3483.
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