Abstract
The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequence. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.
| Original language | English |
|---|---|
| Pages (from-to) | 3479-3483 |
| Number of pages | 5 |
| Journal | Journal of Biological Chemistry |
| Volume | 265 |
| Issue number | 6 |
| Publication status | Published - Feb 25 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription. / Nakagoshi, Hideki; Nagase, Takahiro; Kanei-Ishii, Chie; Ueno, Yoshio; Ishii, Shunsuke.
In: Journal of Biological Chemistry, Vol. 265, No. 6, 25.02.1990, p. 3479-3483.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription
AU - Nakagoshi, Hideki
AU - Nagase, Takahiro
AU - Kanei-Ishii, Chie
AU - Ueno, Yoshio
AU - Ishii, Shunsuke
PY - 1990/2/25
Y1 - 1990/2/25
N2 - The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequence. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.
AB - The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequence. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025255885&partnerID=8YFLogxK
M3 - Article
VL - 265
SP - 3479
EP - 3483
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 6
ER -