Binding of the c-myb proto-oncogene product to the simian virus 40 enhancer stimulates transcription

H. Nakagoshi, T. Nagase, C. Kanei-Ishii, Y. Ueno, S. Ishii

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)

Abstract

The proto-oncogene c-myb encodes a nuclear protein which binds to DNA. Here we find that bacterially synthesized c-myb protein binds to one site of the simian virus 40 enhancer. The c-myb protein purified from the human T-cell line, Molt4, was also shown to recognize the same sequene. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of a c-myb-binding sequence were shown to function as a c-myb-dependent enhancer. These results indicate the c-myb protein is a simian virus 40 enhancer-binding protein that can positively regulate transcription.

Original languageEnglish
Pages (from-to)3479-3483
Number of pages5
JournalJournal of Biological Chemistry
Volume265
Issue number6
Publication statusPublished - Mar 23 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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