Binding of botulinum type B neurotoxin to Chinese hamster ovary cells transfected with rat synaptotagmin II cDNA

Tei Ichi Nishiki, Yoshimi Tokuyama, Yoichi Kamata, Yasuo Nemoto, Akira Yoshida, Mariko Sekiguchi, Masami Takahashi, Shunji Kozaki

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79 Citations (Scopus)

Abstract

We have previously identified synaptotagmin, a synaptic vesicle membrane protein from rat brain, as a binding protein for Clostridium botulinum type B neurotoxin. In this report, rat synaptotagmin II was expressed by transfection in Chinese hamster ovary cells and interaction with the neurotoxin was studied. In stable transfectants, the NH2-terminal region of synaptotagmin was exposed to the extracellular medium. Synaptotagmin-expressing cells were shown to possess an extremely low binding activity for the radioiodinated toxin. However, toxin-binding was markedly increased to cells which had been treated with gangliosides G(T1b) or G(D1a). In synapses, the intravesicular NH2-terminus of synaptotagmin becomes exposed at the cell surface after following exocytosis. These findings suggest that the NH2-terminal domain of synaptotagmin II forms the binding site for type B neurotoxin by associating with specific gangliosides in presynaptic plasma membranes.

Original languageEnglish
Pages (from-to)105-108
Number of pages4
JournalNeuroscience Letters
Volume208
Issue number2
DOIs
Publication statusPublished - Apr 19 1996

Keywords

  • Chinese hamster ovary cells
  • botulinum neurotoxin
  • ganglioside
  • receptor
  • stable transfection
  • synaptotagmin

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Nishiki, T. I., Tokuyama, Y., Kamata, Y., Nemoto, Y., Yoshida, A., Sekiguchi, M., Takahashi, M., & Kozaki, S. (1996). Binding of botulinum type B neurotoxin to Chinese hamster ovary cells transfected with rat synaptotagmin II cDNA. Neuroscience Letters, 208(2), 105-108. https://doi.org/10.1016/0304-3940(96)12557-X