Binding and functional properties of five extrinsic proteins in oxygen-evolving photosystem II from a marine centric diatom, Chaetoceros gracilis

Ryo Nagao, Akira Moriguchi, Tatsuya Tomo, Ayako Niikura, Saori Nakajima, Takehiro Suzuki, Akinori Okumura, Masako Iwai, Jian-Ren Shen, Masahiko Ikeuchi, Isao Enami

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Abstract

Oxygen-evolving photosystem II (PSII) isolated from a marine centric diatom, Chaetoceros gracilis, contains a novel extrinsic protein (Psb31) in addition to four red algal type extrinsic proteins of PsbO, PsbQ′, PsbV, and PsbU. In this study, the five extrinsic proteins were purified from alkaline Tris extracts of the diatom PSII by anion and cation exchange chromatographic columns at different pH values. Reconstitution experiments in various combinations with the purified extrinsic proteins showed that PsbO, PsbQ′, and Psb31 rebound directly to PSII in the absence of other extrinsic proteins, indicating that these extrinsic proteins have their own binding sites in PSII intrinsic proteins. On the other hand, PsbV and PsbU scarcely rebound to PSII alone, and their effective bindings required the presence of all of the other extrinsic proteins. Interestingly, PSII reconstituted with Psb31 alone considerably restored the oxygen evolving activity in the absence of PsbO, indicating that Psb31 serves as a substitute in part for PsbO in supporting oxygen evolution. A significant difference found between PSIIs reconstituted with Psb31 and with PsbO is that the oxygen evolving activity of the former is scarcely stimulated by Cl- and Ca2+ ions but that of the latter is largely stimulated by these ions, although rebinding of PsbV and PsbU activated oxygen evolution in the absence of Cl- and Ca2+ ions in both the former and latter PSIIs. Based on these results, we proposed a model for the association of the five extrinsic proteins with intrinsic proteins in diatom PSII and compared it with those in PSIIs from the other organisms.

Original languageEnglish
Pages (from-to)29191-29199
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number38
DOIs
Publication statusPublished - Sep 17 2010

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Diatoms
Photosystem II Protein Complex
Oxygen
Proteins
Ions
Algal Proteins
Anions
Cations
Binding Sites

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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Binding and functional properties of five extrinsic proteins in oxygen-evolving photosystem II from a marine centric diatom, Chaetoceros gracilis. / Nagao, Ryo; Moriguchi, Akira; Tomo, Tatsuya; Niikura, Ayako; Nakajima, Saori; Suzuki, Takehiro; Okumura, Akinori; Iwai, Masako; Shen, Jian-Ren; Ikeuchi, Masahiko; Enami, Isao.

In: Journal of Biological Chemistry, Vol. 285, No. 38, 17.09.2010, p. 29191-29199.

Research output: Contribution to journalArticle

Nagao, Ryo ; Moriguchi, Akira ; Tomo, Tatsuya ; Niikura, Ayako ; Nakajima, Saori ; Suzuki, Takehiro ; Okumura, Akinori ; Iwai, Masako ; Shen, Jian-Ren ; Ikeuchi, Masahiko ; Enami, Isao. / Binding and functional properties of five extrinsic proteins in oxygen-evolving photosystem II from a marine centric diatom, Chaetoceros gracilis. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 38. pp. 29191-29199.
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AU - Niikura, Ayako

AU - Nakajima, Saori

AU - Suzuki, Takehiro

AU - Okumura, Akinori

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AU - Shen, Jian-Ren

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AU - Enami, Isao

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