Biliary excretion of polystyrene microspheres depends on the type of receptor-mediated uptake in rat liver

Kentaro Furumoto, Ken Ichi Ogawara, Minoru Yoshida, Yoshinobu Takakura, Mitsuru Hashida, Kazutaka Higaki, Toshikiro Kimura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Hepatic uptake and biliary excretion of fluorescein isothiocyanate-labeled polystyrene microspheres with a particle size of 50 nm (MS-50) were studied in rats. Liver perfusion studies revealed that not only apo-E-mediated but also asialoglycoprotein receptor-mediated uptake is involved in the mechanism of the serum protein-dependent uptake of MS-50 in the liver. The uptake of MS-50 mediated by apo-E contributes more to the total uptake of MS-50 by the hepatocytes than that via asialoglycoprotein receptor in the presence of serum in the perfusate. Furthermore, it was found that MS-50 is substantially excreted into the bile by transcytosis. The extent of exocytosis of MS-50 taken up by the hepatocytes was much higher after MS-50 was endocytosed via asialoglycoprotein receptor than after taken up via the process mediated by apo-E. On the basis of these results, a possible regulation of the intracellular sorting of ligands, depending on the receptor-mediated uptake mechanism, was inferred.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalBiochimica et Biophysica Acta - General Subjects
Volume1526
Issue number2
DOIs
Publication statusPublished - May 3 2001

Keywords

  • Apo-E
  • Asialoglycoprotein receptor
  • Hepato-biliary transport
  • Intracellular trafficking
  • Polystyrene microsphere

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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