Biliary bacteria in living related liver transplant recipients: Microbiology and rapid detection system using flow cytometry

Takashi Saito, Kazuyoshi Senda, Shunji Takakura, Naoko Fujihara, Toyoichiro Kudo, Yoshitsugu Iinuma, Tetsuya Kiuchi, Mitsune Tanimoto, Satoshi Ichiyama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Since cholangitis is a major complication in living related liver transplant (LRLT) recipients, rapid detection of biliary bacteria is necessary for the management of patients. We have developed a screening method for the detection of biliary bacteria using flow cytometry (FCM). Two hundred and seventy eight bile samples were obtained from 50 patients with biliary drainage tubes after LRLT at Kyoto University Hospital between July and September 2001. Of the 278 samples, 165 (59.3%) were culture-positive. The most common isolates were Enterococcus species, Pseudomonas species, Staphylococcus species, Klebsiella species, and Candida species. As the original FCM system was inadequate for specifically detecting bacteria in bile samples, we established the most appropriate gate and cut-off value from the particle distribution represented on scattergram of the forward-scattered light and fluorescent light intensity. The 3% cut-off value was most preferably related to the culture results. The FCM system detected biliary bacteria with a sensitivity of 93.9%, specificity of 81.4%, positive predictive value of 88.1%, negative predictive value of 90.2%, false-positive rate of 7.6%, false-negative rate of 3.6%, and percent agreement of 88.9% between FCM and culture. Therefore, FCM can be a useful method in clinical laboratories for the rapid screening for biliary bacteria in LRLT recipients.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalClinical Chemistry and Laboratory Medicine
Volume41
Issue number2
DOIs
Publication statusPublished - 2003

Fingerprint

Microbiology
Transplants
Flow cytometry
Liver
Bacteria
Flow Cytometry
Bile
Screening
Clinical laboratories
Light
Cholangitis
Klebsiella
Candida
Enterococcus
Biliary Tract
Pseudomonas
Staphylococcus
Drainage
Transplant Recipients
Sensitivity and Specificity

Keywords

  • Biliary bacteria
  • Flow cytometry
  • Liver transplantation

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Biliary bacteria in living related liver transplant recipients : Microbiology and rapid detection system using flow cytometry. / Saito, Takashi; Senda, Kazuyoshi; Takakura, Shunji; Fujihara, Naoko; Kudo, Toyoichiro; Iinuma, Yoshitsugu; Kiuchi, Tetsuya; Tanimoto, Mitsune; Ichiyama, Satoshi.

In: Clinical Chemistry and Laboratory Medicine, Vol. 41, No. 2, 2003, p. 159-163.

Research output: Contribution to journalArticle

Saito, T, Senda, K, Takakura, S, Fujihara, N, Kudo, T, Iinuma, Y, Kiuchi, T, Tanimoto, M & Ichiyama, S 2003, 'Biliary bacteria in living related liver transplant recipients: Microbiology and rapid detection system using flow cytometry', Clinical Chemistry and Laboratory Medicine, vol. 41, no. 2, pp. 159-163. https://doi.org/10.1515/CCLM.2003.026
Saito, Takashi ; Senda, Kazuyoshi ; Takakura, Shunji ; Fujihara, Naoko ; Kudo, Toyoichiro ; Iinuma, Yoshitsugu ; Kiuchi, Tetsuya ; Tanimoto, Mitsune ; Ichiyama, Satoshi. / Biliary bacteria in living related liver transplant recipients : Microbiology and rapid detection system using flow cytometry. In: Clinical Chemistry and Laboratory Medicine. 2003 ; Vol. 41, No. 2. pp. 159-163.
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