Bile acids are new products of a marine bacterium, Myroides sp. strain SM1

Suppasil Maneerat, Teruhiko Nitoda, Hiroshi Kanzaki, Fusako Kawai

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Strain SM1 was isolated as a biosurfactant-producing microorganism from seawater and presumptively identified as Myroides sp., based on morphology, biochemical characteristics and 16S rDNA sequence. The strain produced surface-active compounds in marine broth, which were purified, using emulsification activity for n-hexadecane as an indicator. The purified compounds were identified by thin-layer chromatography, 1H- and 13C-NMR spectra and fast atom bombardment mass spectrometry as cholic acid, deoxycholic acid and their glycine conjugates. Type strains of the genus Myroides, M. odoratus JCM7458 and M. odoramitimus JCM7460, also produced these compounds. Myroides sp. strain SM1 possessed a biosynthetic route to cholic acid from cholesterol. Thus, bile acids were found as new products of prokaryotic cells, genus Myroides.

Original languageEnglish
Pages (from-to)679-683
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume67
Issue number5
DOIs
Publication statusPublished - Jun 2005

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Cholic Acid
Bile Acids and Salts
Bacteria
Prokaryotic Cells
Fast Atom Bombardment Mass Spectrometry
Deoxycholic Acid
Acids
Seawater
Thin Layer Chromatography
Ribosomal DNA
Glycine
Cholesterol
Thin layer chromatography
Emulsification
Microorganisms
Mass spectrometry
Amino acids
Nuclear magnetic resonance
Atoms
n-hexadecane

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

Bile acids are new products of a marine bacterium, Myroides sp. strain SM1. / Maneerat, Suppasil; Nitoda, Teruhiko; Kanzaki, Hiroshi; Kawai, Fusako.

In: Applied Microbiology and Biotechnology, Vol. 67, No. 5, 06.2005, p. 679-683.

Research output: Contribution to journalArticle

Maneerat, Suppasil ; Nitoda, Teruhiko ; Kanzaki, Hiroshi ; Kawai, Fusako. / Bile acids are new products of a marine bacterium, Myroides sp. strain SM1. In: Applied Microbiology and Biotechnology. 2005 ; Vol. 67, No. 5. pp. 679-683.
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