Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants

Kazuyoshi Goto, Toshio Kodama, Hirotaka Hiyoshi, Kaori Izutsu, Kwon Sam Park, Rikard Dryselius, Yukihiro Akeda, Takeshi Honda, Tetsuya Iida

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Vibrio parahaemolyticus, a bacterial pathogen, causes human gastroenteritis. A type III secretion system (T3SS2) encoded in pathogenicity island (Vp-PAI) is the main contributor to enterotoxicity and expression of Vp-PAI encoded genes is regulated by two transcriptional regulators, VtrA and VtrB. However, a host-derived inducer for the Vp-PAI genes has not been identified. Here, we demonstrate that bile induces production of T3SS2-related proteins under osmotic conditions equivalent to those in the intestinal lumen. We also show that bile induces vtrA-mediated vtrB transcription. Transcriptome analysis of bile-responsive genes revealed that bile strongly induces expression of Vp-PAI genes in a vtrA-dependent manner. The inducing activity of bile was diminished by treatment with bile acid sequestrant cholestyramine. Finally, we demonstrate an in vivo protective effect of cholestyramine on enterotoxicity and show that similar protection is observed in infection with a different type of V. parahaemolyticus or with non-O1/non-O139 V. cholerae strains of vibrios carrying the same kind of T3SS. In summary, these results provide an insight into how bacteria, through the ingenious action of Vp-PAI genes, can take advantage of an otherwise hostile host environment. The results also reveal a new therapeutic potential for widely used bile acid sequestrants in enteric bacterial infections. Copyright:

Original languageEnglish
Article numbere13365
JournalPLoS One
Volume5
Issue number10
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Polyamideimides
Vibrio parahaemolyticus
bile acids
bile
Bile Acids and Salts
Gene expression
Bile
Virulence
virulence
Genes
Gene Expression
gene expression
therapeutics
cholestyramine
Cholestyramine Resin
type III secretion system
genes
pathogenicity islands
Genomic Islands
Therapeutics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants. / Goto, Kazuyoshi; Kodama, Toshio; Hiyoshi, Hirotaka; Izutsu, Kaori; Park, Kwon Sam; Dryselius, Rikard; Akeda, Yukihiro; Honda, Takeshi; Iida, Tetsuya.

In: PLoS One, Vol. 5, No. 10, e13365, 2010.

Research output: Contribution to journalArticle

Goto, Kazuyoshi ; Kodama, Toshio ; Hiyoshi, Hirotaka ; Izutsu, Kaori ; Park, Kwon Sam ; Dryselius, Rikard ; Akeda, Yukihiro ; Honda, Takeshi ; Iida, Tetsuya. / Bile acid-induced virulence gene expression of Vibrio parahaemolyticus reveals a novel therapeutic potential for bile acid sequestrants. In: PLoS One. 2010 ; Vol. 5, No. 10.
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