Bifidobacteria can protect from enteropathogenic infection through production of acetate

Shinji Fukuda, Hidehiro Toh, Koji Hase, Kenshiro Oshima, Yumiko Nakanishi, Kazutoshi Yoshimura, Toru Tobe, Julie M. Clarke, David L. Topping, Tohru Suzuki, Todd D. Taylor, Kikuji Itoh, Jun Kikuchi, Hidetoshi Morita, Masahira Hattori, Hiroshi Ohno

Research output: Contribution to journalArticle

901 Citations (Scopus)

Abstract

The human gut is colonized with a wide variety of microorganisms, including species, such as those belonging to the bacterial genus Bifidobacterium, that have beneficial effects on human physiology and pathology. Among the most distinctive benefits of bifidobacteria are modulation of host defence responses and protection against infectious diseases. Nevertheless, the molecular mechanisms underlying these effects have barely been elucidated. To investigate these mechanisms, we used mice associated with certain bifidobacterial strains and a simplified model of lethal infection with enterohaemorrhagic Escherichia coli O157:H7, together with an integrated ĝ€̃ omics- approach. Here we show that genes encoding an ATP-binding-cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting mice against death induced by E. coli O157:H7. We found that this effect can be attributed, at least in part, to increased production of acetate and that translocation of the E. coli O157:H7 Shiga toxin from the gut lumen to the blood was inhibited. We propose that acetate produced by protective bifidobacteria improves intestinal defence mediated by epithelial cells and thereby protects the host against lethal infection.

Original languageEnglish
Pages (from-to)543-549
Number of pages7
JournalNature
Volume469
Issue number7331
DOIs
Publication statusPublished - Jan 27 2011
Externally publishedYes

Fingerprint

Bifidobacterium
Escherichia coli O157
Acetates
Infection
Shiga Toxin
Enterohemorrhagic Escherichia coli
ATP-Binding Cassette Transporters
Communicable Diseases
Epithelial Cells
Carbohydrates
Pathology
Genes

ASJC Scopus subject areas

  • General

Cite this

Fukuda, S., Toh, H., Hase, K., Oshima, K., Nakanishi, Y., Yoshimura, K., ... Ohno, H. (2011). Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature, 469(7331), 543-549. https://doi.org/10.1038/nature09646

Bifidobacteria can protect from enteropathogenic infection through production of acetate. / Fukuda, Shinji; Toh, Hidehiro; Hase, Koji; Oshima, Kenshiro; Nakanishi, Yumiko; Yoshimura, Kazutoshi; Tobe, Toru; Clarke, Julie M.; Topping, David L.; Suzuki, Tohru; Taylor, Todd D.; Itoh, Kikuji; Kikuchi, Jun; Morita, Hidetoshi; Hattori, Masahira; Ohno, Hiroshi.

In: Nature, Vol. 469, No. 7331, 27.01.2011, p. 543-549.

Research output: Contribution to journalArticle

Fukuda, S, Toh, H, Hase, K, Oshima, K, Nakanishi, Y, Yoshimura, K, Tobe, T, Clarke, JM, Topping, DL, Suzuki, T, Taylor, TD, Itoh, K, Kikuchi, J, Morita, H, Hattori, M & Ohno, H 2011, 'Bifidobacteria can protect from enteropathogenic infection through production of acetate', Nature, vol. 469, no. 7331, pp. 543-549. https://doi.org/10.1038/nature09646
Fukuda S, Toh H, Hase K, Oshima K, Nakanishi Y, Yoshimura K et al. Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature. 2011 Jan 27;469(7331):543-549. https://doi.org/10.1038/nature09646
Fukuda, Shinji ; Toh, Hidehiro ; Hase, Koji ; Oshima, Kenshiro ; Nakanishi, Yumiko ; Yoshimura, Kazutoshi ; Tobe, Toru ; Clarke, Julie M. ; Topping, David L. ; Suzuki, Tohru ; Taylor, Todd D. ; Itoh, Kikuji ; Kikuchi, Jun ; Morita, Hidetoshi ; Hattori, Masahira ; Ohno, Hiroshi. / Bifidobacteria can protect from enteropathogenic infection through production of acetate. In: Nature. 2011 ; Vol. 469, No. 7331. pp. 543-549.
@article{237fe53bc584448a950b9ba96b9e5ac0,
title = "Bifidobacteria can protect from enteropathogenic infection through production of acetate",
abstract = "The human gut is colonized with a wide variety of microorganisms, including species, such as those belonging to the bacterial genus Bifidobacterium, that have beneficial effects on human physiology and pathology. Among the most distinctive benefits of bifidobacteria are modulation of host defence responses and protection against infectious diseases. Nevertheless, the molecular mechanisms underlying these effects have barely been elucidated. To investigate these mechanisms, we used mice associated with certain bifidobacterial strains and a simplified model of lethal infection with enterohaemorrhagic Escherichia coli O157:H7, together with an integrated ĝ€̃ omics- approach. Here we show that genes encoding an ATP-binding-cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting mice against death induced by E. coli O157:H7. We found that this effect can be attributed, at least in part, to increased production of acetate and that translocation of the E. coli O157:H7 Shiga toxin from the gut lumen to the blood was inhibited. We propose that acetate produced by protective bifidobacteria improves intestinal defence mediated by epithelial cells and thereby protects the host against lethal infection.",
author = "Shinji Fukuda and Hidehiro Toh and Koji Hase and Kenshiro Oshima and Yumiko Nakanishi and Kazutoshi Yoshimura and Toru Tobe and Clarke, {Julie M.} and Topping, {David L.} and Tohru Suzuki and Taylor, {Todd D.} and Kikuji Itoh and Jun Kikuchi and Hidetoshi Morita and Masahira Hattori and Hiroshi Ohno",
year = "2011",
month = "1",
day = "27",
doi = "10.1038/nature09646",
language = "English",
volume = "469",
pages = "543--549",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7331",

}

TY - JOUR

T1 - Bifidobacteria can protect from enteropathogenic infection through production of acetate

AU - Fukuda, Shinji

AU - Toh, Hidehiro

AU - Hase, Koji

AU - Oshima, Kenshiro

AU - Nakanishi, Yumiko

AU - Yoshimura, Kazutoshi

AU - Tobe, Toru

AU - Clarke, Julie M.

AU - Topping, David L.

AU - Suzuki, Tohru

AU - Taylor, Todd D.

AU - Itoh, Kikuji

AU - Kikuchi, Jun

AU - Morita, Hidetoshi

AU - Hattori, Masahira

AU - Ohno, Hiroshi

PY - 2011/1/27

Y1 - 2011/1/27

N2 - The human gut is colonized with a wide variety of microorganisms, including species, such as those belonging to the bacterial genus Bifidobacterium, that have beneficial effects on human physiology and pathology. Among the most distinctive benefits of bifidobacteria are modulation of host defence responses and protection against infectious diseases. Nevertheless, the molecular mechanisms underlying these effects have barely been elucidated. To investigate these mechanisms, we used mice associated with certain bifidobacterial strains and a simplified model of lethal infection with enterohaemorrhagic Escherichia coli O157:H7, together with an integrated ĝ€̃ omics- approach. Here we show that genes encoding an ATP-binding-cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting mice against death induced by E. coli O157:H7. We found that this effect can be attributed, at least in part, to increased production of acetate and that translocation of the E. coli O157:H7 Shiga toxin from the gut lumen to the blood was inhibited. We propose that acetate produced by protective bifidobacteria improves intestinal defence mediated by epithelial cells and thereby protects the host against lethal infection.

AB - The human gut is colonized with a wide variety of microorganisms, including species, such as those belonging to the bacterial genus Bifidobacterium, that have beneficial effects on human physiology and pathology. Among the most distinctive benefits of bifidobacteria are modulation of host defence responses and protection against infectious diseases. Nevertheless, the molecular mechanisms underlying these effects have barely been elucidated. To investigate these mechanisms, we used mice associated with certain bifidobacterial strains and a simplified model of lethal infection with enterohaemorrhagic Escherichia coli O157:H7, together with an integrated ĝ€̃ omics- approach. Here we show that genes encoding an ATP-binding-cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting mice against death induced by E. coli O157:H7. We found that this effect can be attributed, at least in part, to increased production of acetate and that translocation of the E. coli O157:H7 Shiga toxin from the gut lumen to the blood was inhibited. We propose that acetate produced by protective bifidobacteria improves intestinal defence mediated by epithelial cells and thereby protects the host against lethal infection.

UR - http://www.scopus.com/inward/record.url?scp=79251584066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251584066&partnerID=8YFLogxK

U2 - 10.1038/nature09646

DO - 10.1038/nature09646

M3 - Article

C2 - 21270894

AN - SCOPUS:79251584066

VL - 469

SP - 543

EP - 549

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7331

ER -