Bifemelane hydrochloride protects against cytotoxicity of hydrogen peroxide on cultured rat neuroblastoma cell line

Ikuko Miyazaki, Emi Iwata-Ichikawa, Masato Asanuma, Motoyuki Iida, Norio Ogawa

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Free radicals are involved in neuronal damage. Bifemelane hydrochloride has been reported to protect neural tissues against ischemic damage and age- related neurodegeneration. We examined the protective effects of bifemelane HCl and the relation between its effectiveness and free radical formation in hydrogen peroxide (H2O2)-induced cytotoxicity using cultured rat neuroblastoma cell line (B50). Cytotoxicity was examined by using the lactate dehydrogenase (LDH) assay and cell viability by the WST-1 assay H2O2 reduced the survival of B50 cells in a dose-dependent manner, and treatment of these cells with 75 μM or 100 μM H2O2 reduced their viability by 50% relative to the control group. B50 cells were treated with 5 or 10 μM bifemelane for 2 days followed by treatment with 75 μM or 100 μM H2O2. H2O2 cytotoxicity was reduced by pretreatment with bifemelane. We also examined the effect of bifemelane on lipid peroxide formation in B50 cells using thiobarbituric acid reactive substances assay. Pretreatment of B50 cells with 10 μM bifemelane for 2 days reduced lipid peroxide formation to approximately 54% of the control group. Our results suggest that bifemelane hydrochloride provides a protective effect against H2O2 cytotoxicity partly due to its anti-oxidative properties.

Original languageEnglish
Pages (from-to)857-860
Number of pages4
JournalNeurochemical Research
Issue number7
Publication statusPublished - 1999



  • Bifemelane hydrochloride
  • Cell culture
  • Hydrogen peroxide
  • Neuroprotection
  • Oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

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