Bicarbonate stimulates the electron donation from Mn2+ to P 680 + in isolated D1/D2/cytochrome b559 complex

S. K. Zharmukhamedov, Suleyman Allakhverdiev, T. N. Smolova, V. V. Klimov

Research output: Contribution to journalArticle

Abstract

Influence of bicarbonate on the efficiency of the electron donation from Mn2+ to P680+ in isolated D1/D2/cytochrome b559 complex was investigated. All the experiments were carried out in a medium depleted of HCO3-/CO2. Kinetics of photoinduced absorbance changes (ΔA) at different wavelengths and decrease of chlorophyll fluorescence yield (-ΔF) related to photoaccumulation of reduced pheophytin, the intermediary electron acceptor of photosystem II (PSII), in the presence of Mn2+ under anaerobic conditions were measured. Addition of bicarbonate (1 mM) increased the amplitude of these ΔA and -ΔF at least by a factor of 3. Measurements of the photoinduced ΔA, related to photooxidation of the primary electron donor of PSII, chlorophyll P680, were done in the presence of silicomolybdate as electron acceptor. These results show that the addition of 0.05 mM Mn2+ alone or jointly with 1 mM bicarbonate induces a 20% and 70%-decrease of the magnitude of the ΔA at 680 nm. The effect of Mn2+ (in the presence and absence of bicarbonate) was completely eliminated by the addition of 12 mM EDTA. All these bicarbonate effects were not observed if MgCl2 or formate were used instead of MnCl2 and bicarbonate, respectively. In the absence of Mn 2+, bicarbonate induced none of the mentioned above effects (increase of photoaccumulation of reduced pheophytin and decrease of photooxidation of P680). The presented data suggest that bicarbonate stimulates the electron donation from Mn2+ to D1/D2/cyt b559 reaction center evidently due to formation of easily oxidizable Mn-bicarbonate complexes.

Original languageEnglish
Pages (from-to)87-92
Number of pages6
JournalJournal of Photochemistry and Photobiology B: Biology
Volume129
DOIs
Publication statusPublished - Nov 12 2013
Externally publishedYes

Fingerprint

cytochromes
Bicarbonates
carbonates
Electrons
electrons
Pheophytins
formic acid
Photosystem II Protein Complex
photooxidation
chlorophylls
Chlorophyll
P-680
cytochrome b559
Magnesium Chloride
ethylenediaminetetraacetic acids
formates
Edetic Acid
Fluorescence
fluorescence
kinetics

Keywords

  • Bicarbonate
  • Manganese
  • Photosystem II
  • Reaction center
  • Silicomolybdate

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Bicarbonate stimulates the electron donation from Mn2+ to P 680 + in isolated D1/D2/cytochrome b559 complex. / Zharmukhamedov, S. K.; Allakhverdiev, Suleyman; Smolova, T. N.; Klimov, V. V.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 129, 12.11.2013, p. 87-92.

Research output: Contribution to journalArticle

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abstract = "Influence of bicarbonate on the efficiency of the electron donation from Mn2+ to P680+ in isolated D1/D2/cytochrome b559 complex was investigated. All the experiments were carried out in a medium depleted of HCO3-/CO2. Kinetics of photoinduced absorbance changes (ΔA) at different wavelengths and decrease of chlorophyll fluorescence yield (-ΔF) related to photoaccumulation of reduced pheophytin, the intermediary electron acceptor of photosystem II (PSII), in the presence of Mn2+ under anaerobic conditions were measured. Addition of bicarbonate (1 mM) increased the amplitude of these ΔA and -ΔF at least by a factor of 3. Measurements of the photoinduced ΔA, related to photooxidation of the primary electron donor of PSII, chlorophyll P680, were done in the presence of silicomolybdate as electron acceptor. These results show that the addition of 0.05 mM Mn2+ alone or jointly with 1 mM bicarbonate induces a 20{\%} and 70{\%}-decrease of the magnitude of the ΔA at 680 nm. The effect of Mn2+ (in the presence and absence of bicarbonate) was completely eliminated by the addition of 12 mM EDTA. All these bicarbonate effects were not observed if MgCl2 or formate were used instead of MnCl2 and bicarbonate, respectively. In the absence of Mn 2+, bicarbonate induced none of the mentioned above effects (increase of photoaccumulation of reduced pheophytin and decrease of photooxidation of P680). The presented data suggest that bicarbonate stimulates the electron donation from Mn2+ to D1/D2/cyt b559 reaction center evidently due to formation of easily oxidizable Mn-bicarbonate complexes.",
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T1 - Bicarbonate stimulates the electron donation from Mn2+ to P 680 + in isolated D1/D2/cytochrome b559 complex

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AU - Klimov, V. V.

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N2 - Influence of bicarbonate on the efficiency of the electron donation from Mn2+ to P680+ in isolated D1/D2/cytochrome b559 complex was investigated. All the experiments were carried out in a medium depleted of HCO3-/CO2. Kinetics of photoinduced absorbance changes (ΔA) at different wavelengths and decrease of chlorophyll fluorescence yield (-ΔF) related to photoaccumulation of reduced pheophytin, the intermediary electron acceptor of photosystem II (PSII), in the presence of Mn2+ under anaerobic conditions were measured. Addition of bicarbonate (1 mM) increased the amplitude of these ΔA and -ΔF at least by a factor of 3. Measurements of the photoinduced ΔA, related to photooxidation of the primary electron donor of PSII, chlorophyll P680, were done in the presence of silicomolybdate as electron acceptor. These results show that the addition of 0.05 mM Mn2+ alone or jointly with 1 mM bicarbonate induces a 20% and 70%-decrease of the magnitude of the ΔA at 680 nm. The effect of Mn2+ (in the presence and absence of bicarbonate) was completely eliminated by the addition of 12 mM EDTA. All these bicarbonate effects were not observed if MgCl2 or formate were used instead of MnCl2 and bicarbonate, respectively. In the absence of Mn 2+, bicarbonate induced none of the mentioned above effects (increase of photoaccumulation of reduced pheophytin and decrease of photooxidation of P680). The presented data suggest that bicarbonate stimulates the electron donation from Mn2+ to D1/D2/cyt b559 reaction center evidently due to formation of easily oxidizable Mn-bicarbonate complexes.

AB - Influence of bicarbonate on the efficiency of the electron donation from Mn2+ to P680+ in isolated D1/D2/cytochrome b559 complex was investigated. All the experiments were carried out in a medium depleted of HCO3-/CO2. Kinetics of photoinduced absorbance changes (ΔA) at different wavelengths and decrease of chlorophyll fluorescence yield (-ΔF) related to photoaccumulation of reduced pheophytin, the intermediary electron acceptor of photosystem II (PSII), in the presence of Mn2+ under anaerobic conditions were measured. Addition of bicarbonate (1 mM) increased the amplitude of these ΔA and -ΔF at least by a factor of 3. Measurements of the photoinduced ΔA, related to photooxidation of the primary electron donor of PSII, chlorophyll P680, were done in the presence of silicomolybdate as electron acceptor. These results show that the addition of 0.05 mM Mn2+ alone or jointly with 1 mM bicarbonate induces a 20% and 70%-decrease of the magnitude of the ΔA at 680 nm. The effect of Mn2+ (in the presence and absence of bicarbonate) was completely eliminated by the addition of 12 mM EDTA. All these bicarbonate effects were not observed if MgCl2 or formate were used instead of MnCl2 and bicarbonate, respectively. In the absence of Mn 2+, bicarbonate induced none of the mentioned above effects (increase of photoaccumulation of reduced pheophytin and decrease of photooxidation of P680). The presented data suggest that bicarbonate stimulates the electron donation from Mn2+ to D1/D2/cyt b559 reaction center evidently due to formation of easily oxidizable Mn-bicarbonate complexes.

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