Bicarbonate-reversible formate inhibition at the donor side of photosystem II

Hanna Wincencjusz, Suleyman I. Allakhverdiev, Vyacheslav V. Klimov, Hans J. Van Gorkom

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Flash-induced absorbance changes at 295 nm show that in the spinach Photosystem II preparation called DT-20 the four-step redox cycle of the oxygen evolving complex is blocked by very low formate concentrations, 50% at 10 μM, and fully restored by a 2-fold higher bicarbonate concentration. These concentrations are four orders of magnitude lower than those involved in the well-known acceptor-side inhibition by formate and did not affect 295 nm absorbance changes in thylakoids, 'BBY' PS II membrane fragments, or PS II core particles. The effect is not related to the chloride dependence of oxygen evolution and appears to be an all-or-none inhibition already present on the first flash.

Original languageEnglish
Pages (from-to)1-3
Number of pages3
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1273
Issue number1
DOIs
Publication statusPublished - Jan 11 1996

Keywords

  • Bicarbonate
  • Formate inhibition
  • Oxygen evolution
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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