Abstract
Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 μM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate effect' on the acceptor side of photosystem II. In preparations depleted of manganese the restoration of electron flow with 0.1-0.2 μM MnCl2 (2-4 Mn per photosystem II reaction center) occurs only in the presence of bicarbonate and it is accompanied by an increased functional binding of manganese. Restoration of electron flow with diphenylcarbazide or NH2OH does not require the addition of NaHCO3. It is suggested that bicarbonate participates in the formation of the Mn-cluster capable of water oxidation or serves as a substrate for the water-oxidizing center.
| Original language | English |
|---|---|
| Pages (from-to) | 251-255 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 363 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Apr 24 1995 |
| Externally published | Yes |
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Keywords
- Bicarbonate
- Donor side
- Manganese
- Photosystem II
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Bicarbonate requirement for the donor side of photosystem II. / Klimov, V. V.; Allakhverdiev, Suleyman; Feyziev, Ya M.; Baranov, S. V.
In: FEBS Letters, Vol. 363, No. 3, 24.04.1995, p. 251-255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bicarbonate requirement for the donor side of photosystem II
AU - Klimov, V. V.
AU - Allakhverdiev, Suleyman
AU - Feyziev, Ya M.
AU - Baranov, S. V.
PY - 1995/4/24
Y1 - 1995/4/24
N2 - Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 μM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate effect' on the acceptor side of photosystem II. In preparations depleted of manganese the restoration of electron flow with 0.1-0.2 μM MnCl2 (2-4 Mn per photosystem II reaction center) occurs only in the presence of bicarbonate and it is accompanied by an increased functional binding of manganese. Restoration of electron flow with diphenylcarbazide or NH2OH does not require the addition of NaHCO3. It is suggested that bicarbonate participates in the formation of the Mn-cluster capable of water oxidation or serves as a substrate for the water-oxidizing center.
AB - Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 μM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate effect' on the acceptor side of photosystem II. In preparations depleted of manganese the restoration of electron flow with 0.1-0.2 μM MnCl2 (2-4 Mn per photosystem II reaction center) occurs only in the presence of bicarbonate and it is accompanied by an increased functional binding of manganese. Restoration of electron flow with diphenylcarbazide or NH2OH does not require the addition of NaHCO3. It is suggested that bicarbonate participates in the formation of the Mn-cluster capable of water oxidation or serves as a substrate for the water-oxidizing center.
KW - Bicarbonate
KW - Donor side
KW - Manganese
KW - Photosystem II
UR - http://www.scopus.com/inward/record.url?scp=0028959530&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028959530&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(95)00327-6
DO - 10.1016/0014-5793(95)00327-6
M3 - Article
C2 - 7737410
AN - SCOPUS:0028959530
VL - 363
SP - 251
EP - 255
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -