Bicarbonate requirement for the donor side of photosystem II

V. V. Klimov; S. I. Allakhverdiev; Ya M. Feyziev; S. V. Baranov

doi:10.1016/0014-5793(95)00327-6

Bicarbonate requirement for the donor side of photosystem II

V. V. Klimov, S. I. Allakhverdiev, Ya M. Feyziev, S. V. Baranov

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article

76 Citations (Scopus)

Abstract

Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO₃) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 μM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate effect' on the acceptor side of photosystem II. In preparations depleted of manganese the restoration of electron flow with 0.1-0.2 μM MnCl₂ (2-4 Mn per photosystem II reaction center) occurs only in the presence of bicarbonate and it is accompanied by an increased functional binding of manganese. Restoration of electron flow with diphenylcarbazide or NH₂OH does not require the addition of NaHCO₃. It is suggested that bicarbonate participates in the formation of the Mn-cluster capable of water oxidation or serves as a substrate for the water-oxidizing center.

Original language	English
Pages (from-to)	251-255
Number of pages	5
Journal	FEBS Letters
Volume	363
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)00327-6
Publication status	Published - Apr 24 1995

Keywords

Bicarbonate
Donor side
Manganese
Photosystem II

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Klimov, V. V., Allakhverdiev, S. I., Feyziev, Y. M., & Baranov, S. V. (1995). Bicarbonate requirement for the donor side of photosystem II. FEBS Letters, 363(3), 251-255. https://doi.org/10.1016/0014-5793(95)00327-6

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abstract = "Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 μM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate effect' on the acceptor side of photosystem II. In preparations depleted of manganese the restoration of electron flow with 0.1-0.2 μM MnCl2 (2-4 Mn per photosystem II reaction center) occurs only in the presence of bicarbonate and it is accompanied by an increased functional binding of manganese. Restoration of electron flow with diphenylcarbazide or NH2OH does not require the addition of NaHCO3. It is suggested that bicarbonate participates in the formation of the Mn-cluster capable of water oxidation or serves as a substrate for the water-oxidizing center.",

keywords = "Bicarbonate, Donor side, Manganese, Photosystem II",

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AU - Klimov, V. V.

AU - Allakhverdiev, S. I.

AU - Feyziev, Ya M.

AU - Baranov, S. V.

PY - 1995/4/24

Y1 - 1995/4/24

N2 - Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 μM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate effect' on the acceptor side of photosystem II. In preparations depleted of manganese the restoration of electron flow with 0.1-0.2 μM MnCl2 (2-4 Mn per photosystem II reaction center) occurs only in the presence of bicarbonate and it is accompanied by an increased functional binding of manganese. Restoration of electron flow with diphenylcarbazide or NH2OH does not require the addition of NaHCO3. It is suggested that bicarbonate participates in the formation of the Mn-cluster capable of water oxidation or serves as a substrate for the water-oxidizing center.

AB - Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 μM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate effect' on the acceptor side of photosystem II. In preparations depleted of manganese the restoration of electron flow with 0.1-0.2 μM MnCl2 (2-4 Mn per photosystem II reaction center) occurs only in the presence of bicarbonate and it is accompanied by an increased functional binding of manganese. Restoration of electron flow with diphenylcarbazide or NH2OH does not require the addition of NaHCO3. It is suggested that bicarbonate participates in the formation of the Mn-cluster capable of water oxidation or serves as a substrate for the water-oxidizing center.

KW - Bicarbonate

KW - Donor side

KW - Manganese

KW - Photosystem II

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