Bicarbonate requirement for the donor side of photosystem II

V. V. Klimov, Suleyman Allakhverdiev, Ya M. Feyziev, S. V. Baranov

Research output: Contribution to journalArticle

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Abstract

Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 μM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate effect' on the acceptor side of photosystem II. In preparations depleted of manganese the restoration of electron flow with 0.1-0.2 μM MnCl2 (2-4 Mn per photosystem II reaction center) occurs only in the presence of bicarbonate and it is accompanied by an increased functional binding of manganese. Restoration of electron flow with diphenylcarbazide or NH2OH does not require the addition of NaHCO3. It is suggested that bicarbonate participates in the formation of the Mn-cluster capable of water oxidation or serves as a substrate for the water-oxidizing center.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalFEBS Letters
Volume363
Issue number3
DOIs
Publication statusPublished - Apr 24 1995
Externally publishedYes

Fingerprint

formic acid
Photosystem II Protein Complex
Bicarbonates
Restoration
Electrons
Manganese
Diphenylcarbazide
Water
Peas
Membranes
Oxidation
Substrates

Keywords

  • Bicarbonate
  • Donor side
  • Manganese
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Klimov, V. V., Allakhverdiev, S., Feyziev, Y. M., & Baranov, S. V. (1995). Bicarbonate requirement for the donor side of photosystem II. FEBS Letters, 363(3), 251-255. https://doi.org/10.1016/0014-5793(95)00327-6

Bicarbonate requirement for the donor side of photosystem II. / Klimov, V. V.; Allakhverdiev, Suleyman; Feyziev, Ya M.; Baranov, S. V.

In: FEBS Letters, Vol. 363, No. 3, 24.04.1995, p. 251-255.

Research output: Contribution to journalArticle

Klimov, VV, Allakhverdiev, S, Feyziev, YM & Baranov, SV 1995, 'Bicarbonate requirement for the donor side of photosystem II', FEBS Letters, vol. 363, no. 3, pp. 251-255. https://doi.org/10.1016/0014-5793(95)00327-6
Klimov VV, Allakhverdiev S, Feyziev YM, Baranov SV. Bicarbonate requirement for the donor side of photosystem II. FEBS Letters. 1995 Apr 24;363(3):251-255. https://doi.org/10.1016/0014-5793(95)00327-6
Klimov, V. V. ; Allakhverdiev, Suleyman ; Feyziev, Ya M. ; Baranov, S. V. / Bicarbonate requirement for the donor side of photosystem II. In: FEBS Letters. 1995 ; Vol. 363, No. 3. pp. 251-255.
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