Bicarbonate may be required for ligation of manganese in the oxygen- evolving complex of photosystem II

V. V. Klimov, R. J. Hulsebosch, Suleyman Allakhverdiev, H. Wincencjusz, H. J. Van Gorkom, A. J. Hoff

Research output: Contribution to journalArticle

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Abstract

It was previously shown in the photosystem II membrane preparation DT- 20 that photoxidation of the oxygen-evolving manganese cluster was blocked by 0.1 mM formate, unless 0.2 mM bicarbonate was present as well [Wincenejusz, H., Allakhverdiev. S. I., Klimov, V. V., and Van Gorkom, H. J. (1996) Biochim. Biophys. Acta 1273, 1-3]. Here it is shown by measurements of EPR signal II that oxidation of the secondary electron donor, Y(Z), is not inhibited. However, the reduction of Y+/(Z) is greatly slowed and occurs largely by back reaction with reduced acceptors. Bicarbonate is shown to prevent the loss of fast electron donation to Y+(Z). The release of about one or two free Mn2+ per photosystem II during formate treatment, and the fact that these effects are mimicked by Mn-depletion, suggests that formate may act by replacing a bicarbonate which is essential for Mn binding. Irreversible light-induced rebinding in an EPR-silent form of Mn2+ that was added to Mn-depleted DT-20 was indeed found to depend on the presence of bicarbonate, as did the reconstitution in such material of both the fast electron donation to Y+/(Z) and the UV absorbance changes characteristic of a functional oxygen-evolving complex. It is concluded that bicarbonate may be an essential ligand of the functional Mn cluster.

Original languageEnglish
Pages (from-to)16277-16281
Number of pages5
JournalBiochemistry
Volume36
Issue number51
DOIs
Publication statusPublished - Dec 1 1997
Externally publishedYes

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formic acid
Photosystem II Protein Complex
Manganese
Bicarbonates
Ligation
Oxygen
Electrons
Paramagnetic resonance
Ligands
Membranes
Light
Oxidation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Klimov, V. V., Hulsebosch, R. J., Allakhverdiev, S., Wincencjusz, H., Van Gorkom, H. J., & Hoff, A. J. (1997). Bicarbonate may be required for ligation of manganese in the oxygen- evolving complex of photosystem II. Biochemistry, 36(51), 16277-16281. https://doi.org/10.1021/bi9717688

Bicarbonate may be required for ligation of manganese in the oxygen- evolving complex of photosystem II. / Klimov, V. V.; Hulsebosch, R. J.; Allakhverdiev, Suleyman; Wincencjusz, H.; Van Gorkom, H. J.; Hoff, A. J.

In: Biochemistry, Vol. 36, No. 51, 01.12.1997, p. 16277-16281.

Research output: Contribution to journalArticle

Klimov, VV, Hulsebosch, RJ, Allakhverdiev, S, Wincencjusz, H, Van Gorkom, HJ & Hoff, AJ 1997, 'Bicarbonate may be required for ligation of manganese in the oxygen- evolving complex of photosystem II', Biochemistry, vol. 36, no. 51, pp. 16277-16281. https://doi.org/10.1021/bi9717688
Klimov VV, Hulsebosch RJ, Allakhverdiev S, Wincencjusz H, Van Gorkom HJ, Hoff AJ. Bicarbonate may be required for ligation of manganese in the oxygen- evolving complex of photosystem II. Biochemistry. 1997 Dec 1;36(51):16277-16281. https://doi.org/10.1021/bi9717688
Klimov, V. V. ; Hulsebosch, R. J. ; Allakhverdiev, Suleyman ; Wincencjusz, H. ; Van Gorkom, H. J. ; Hoff, A. J. / Bicarbonate may be required for ligation of manganese in the oxygen- evolving complex of photosystem II. In: Biochemistry. 1997 ; Vol. 36, No. 51. pp. 16277-16281.
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