Bicarbonate is an essential constituent of the water-oxidizing complex of photosystem II

Suleyman Allakhverdiev, I. Yruela, R. Picorel, V. V. Klimov

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

It is shown that restoration of photoinduced electron flow and O2 evolution with Mn2+ in Mn-depleted photosystem II (PSII) membrane fragments isolated from spinach chloroplasts is considerably increased with bicarbonate in the region pH 5.0-8.0 in bicarbonate-depleted medium. In buffered solutions equilibrated with the atmosphere (nondepleted of bicarbonate), the bicarbonate effect is observed only at pH lower than the pK of H2CO3 dissociation (6.4), which indicates that HCO3/- is the essential species for the restoration effect. The addition of just 2 Mn2+ atoms per one PSII reaction center is enough for the maximal reactivation when bicarbonate is present in the medium. Analysis of bicarbonate concentration dependence of the restoration effect reveals two binding sites for bicarbonate with apparent dissociation constant (K(d)) of ≃2.5 μM and 20-34 μM when 2,6- dichloro-p-benzoquinone is used as electron acceptor, while in the presence of silicomolybdate only the latter one remains. Similar bicarbonate concentration dependence of O2 evolution was obtained in untreated Mn- containing PSII membrane fragments. It is suggested that the K(d) of 20-34 μM is associated with the donor side of PSII while the location of the lower K(d) binding site is not quite clear. The conclusion is made that bicarbonate is an essential constituent of the water-oxidizing complex of PSII, important for its assembly and maintenance in the functionally active state.

Original languageEnglish
Pages (from-to)5050-5054
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number10
DOIs
Publication statusPublished - May 13 1997
Externally publishedYes

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Photosystem II Protein Complex
Bicarbonates
Water
Binding Sites
Electrons
Spinacia oleracea
Membranes
Chloroplasts
Atmosphere
Maintenance

Keywords

  • donor side
  • manganese
  • oxygen evolution

ASJC Scopus subject areas

  • General

Cite this

Bicarbonate is an essential constituent of the water-oxidizing complex of photosystem II. / Allakhverdiev, Suleyman; Yruela, I.; Picorel, R.; Klimov, V. V.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 10, 13.05.1997, p. 5050-5054.

Research output: Contribution to journalArticle

Allakhverdiev, Suleyman ; Yruela, I. ; Picorel, R. ; Klimov, V. V. / Bicarbonate is an essential constituent of the water-oxidizing complex of photosystem II. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 10. pp. 5050-5054.
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