Abstract
Benzyl isothiocyanate (BITC) is an organosulfur compound derived from cruciferous vegetables and papaya seeds. In this study, we investigated the effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. The treatment of BITC during the differentiation-inducing stage significantly ameliorated the lipid accumulation, whereas it had no inhibitory effect during the differentiation-maintaining stage. BITC also significantly suppressed the mRNA expression of the adipocyte-specific markers, such as CCAAT/enhancer-binding protein α (C/EBPα), C/EBPβ, C/EBPδ and peroxisome proliferator-activated receptor γ. BITC significantly inhibited the phosphorylation of extracellular signal-regulated kinase phosphorylation, whereas it enhanced that of AMP-activated protein kinase. Furthermore, BITC significantly suppressed the intracellular 2-deoxyglucose uptake as well as glucose transporter 4 expression. These results suggest that inhibition of the adipocyte differentiation and glucose uptake may mainly contribute to the inhibitory effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 2130-2139 |
| Number of pages | 10 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 82 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Jan 1 2018 |
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Keywords
- Adipocyte
- Adipogenesis
- Benzyl isothiocyanate
- Differentiation
- GLUT4
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Benzyl isothiocyanate ameliorates lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. / Liang, Ying; Sasaki, Ikumi; Takeda, Yuki; Zhu, Beiwei; Munemasa, Shintaro; Nakamura, Toshiyuki; Murata, Yoshiyuki; Nakamura, Yoshimasa.
In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 12, 01.01.2018, p. 2130-2139.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Benzyl isothiocyanate ameliorates lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation
AU - Liang, Ying
AU - Sasaki, Ikumi
AU - Takeda, Yuki
AU - Zhu, Beiwei
AU - Munemasa, Shintaro
AU - Nakamura, Toshiyuki
AU - Murata, Yoshiyuki
AU - Nakamura, Yoshimasa
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Benzyl isothiocyanate (BITC) is an organosulfur compound derived from cruciferous vegetables and papaya seeds. In this study, we investigated the effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. The treatment of BITC during the differentiation-inducing stage significantly ameliorated the lipid accumulation, whereas it had no inhibitory effect during the differentiation-maintaining stage. BITC also significantly suppressed the mRNA expression of the adipocyte-specific markers, such as CCAAT/enhancer-binding protein α (C/EBPα), C/EBPβ, C/EBPδ and peroxisome proliferator-activated receptor γ. BITC significantly inhibited the phosphorylation of extracellular signal-regulated kinase phosphorylation, whereas it enhanced that of AMP-activated protein kinase. Furthermore, BITC significantly suppressed the intracellular 2-deoxyglucose uptake as well as glucose transporter 4 expression. These results suggest that inhibition of the adipocyte differentiation and glucose uptake may mainly contribute to the inhibitory effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes.
AB - Benzyl isothiocyanate (BITC) is an organosulfur compound derived from cruciferous vegetables and papaya seeds. In this study, we investigated the effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. The treatment of BITC during the differentiation-inducing stage significantly ameliorated the lipid accumulation, whereas it had no inhibitory effect during the differentiation-maintaining stage. BITC also significantly suppressed the mRNA expression of the adipocyte-specific markers, such as CCAAT/enhancer-binding protein α (C/EBPα), C/EBPβ, C/EBPδ and peroxisome proliferator-activated receptor γ. BITC significantly inhibited the phosphorylation of extracellular signal-regulated kinase phosphorylation, whereas it enhanced that of AMP-activated protein kinase. Furthermore, BITC significantly suppressed the intracellular 2-deoxyglucose uptake as well as glucose transporter 4 expression. These results suggest that inhibition of the adipocyte differentiation and glucose uptake may mainly contribute to the inhibitory effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes.
KW - Adipocyte
KW - Adipogenesis
KW - Benzyl isothiocyanate
KW - Differentiation
KW - GLUT4
UR - http://www.scopus.com/inward/record.url?scp=85056355393&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056355393&partnerID=8YFLogxK
U2 - 10.1080/09168451.2018.1514247
DO - 10.1080/09168451.2018.1514247
M3 - Article
C2 - 30185113
AN - SCOPUS:85056355393
VL - 82
SP - 2130
EP - 2139
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 12
ER -