Benzyl isothiocyanate ameliorates lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation

Ying Liang, Ikumi Sasaki, Yuki Takeda, Beiwei Zhu, Shintaro Munemasa, Toshiyuki Nakamura, Yoshiyuki Murata, Yoshimasa Nakamura

Research output: Contribution to journalArticle

Abstract

Benzyl isothiocyanate (BITC) is an organosulfur compound derived from cruciferous vegetables and papaya seeds. In this study, we investigated the effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. The treatment of BITC during the differentiation-inducing stage significantly ameliorated the lipid accumulation, whereas it had no inhibitory effect during the differentiation-maintaining stage. BITC also significantly suppressed the mRNA expression of the adipocyte-specific markers, such as CCAAT/enhancer-binding protein α (C/EBPα), C/EBPβ, C/EBPδ and peroxisome proliferator-activated receptor γ. BITC significantly inhibited the phosphorylation of extracellular signal-regulated kinase phosphorylation, whereas it enhanced that of AMP-activated protein kinase. Furthermore, BITC significantly suppressed the intracellular 2-deoxyglucose uptake as well as glucose transporter 4 expression. These results suggest that inhibition of the adipocyte differentiation and glucose uptake may mainly contribute to the inhibitory effect of BITC on the lipid accumulation in 3T3-L1 preadipocytes.

Original languageEnglish
Pages (from-to)2130-2139
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number12
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Adipocytes
Lipids
CCAAT-Enhancer-Binding Proteins
Phosphorylation
Carica
Peroxisome Proliferator-Activated Receptors
AMP-Activated Protein Kinases
Facilitative Glucose Transport Proteins
Extracellular Signal-Regulated MAP Kinases
Deoxyglucose
Vegetables
benzyl isothiocyanate
Seed
Seeds
Glucose
Messenger RNA

Keywords

  • Adipocyte
  • Adipogenesis
  • Benzyl isothiocyanate
  • Differentiation
  • GLUT4

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Benzyl isothiocyanate ameliorates lipid accumulation in 3T3-L1 preadipocytes during adipocyte differentiation. / Liang, Ying; Sasaki, Ikumi; Takeda, Yuki; Zhu, Beiwei; Munemasa, Shintaro; Nakamura, Toshiyuki; Murata, Yoshiyuki; Nakamura, Yoshimasa.

In: Bioscience, Biotechnology and Biochemistry, Vol. 82, No. 12, 01.01.2018, p. 2130-2139.

Research output: Contribution to journalArticle

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