Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon

Yusuke Kouzai, Yoshiteru Noutoshi, Komaki Inoue, Minami Shimizu, Yoshihiko Onda, Keiichi Mochida

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Plant defense inducers that mimic functions of the plant immune hormone salicylic acid (SA) often affect plant growth. Although benzothiadiazole (BTH), a synthetic analog of SA, has been widely used to protect crops from diseases by inducing plant defense responses, we recently demonstrated that SA, but not BTH, confers resistance against Rhizoctonia solani, the causal agent of sheath blight disease, in Brachypodium distachyon. Here, we demonstrated that BTH compromised the resistance of Bd3-1 and Gaz4, the two sheath blight-resistant accessions of B. distachyon, which activate SA-dependent signaling following challenge by R. solani. Moreover, upon analyzing our published RNA-seq data from B. distachyon treated with SA or BTH, we found that BTH specifically induces expression of genes related to chloroplast function and jasmonic acid (JA) signaling, suggesting that BTH attenuates R. solani resistance by perturbing growth-defense trade-offs and/or by inducing a JA response that may increase susceptibility to R. solani. Our findings demonstrated that BTH does not work as a simple mimic of SA in B. distachyon, and consequently may presumably cause unfavorable side effects through the transcriptional alteration, particularly with respect to R. solani resistance.

Original languageEnglish
Article number17358
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Brachypodium
Salicylic Acid
Rhizoctonia
Plant Diseases
Plant Growth Regulators
Chloroplasts
Growth
benzo-1,2,3-thiadiazole
RNA
Gene Expression

ASJC Scopus subject areas

  • General

Cite this

Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon. / Kouzai, Yusuke; Noutoshi, Yoshiteru; Inoue, Komaki; Shimizu, Minami; Onda, Yoshihiko; Mochida, Keiichi.

In: Scientific Reports, Vol. 8, No. 1, 17358, 01.12.2018.

Research output: Contribution to journalArticle

@article{3850c3d420af441c9f2b683789e2e0b1,
title = "Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon",
abstract = "Plant defense inducers that mimic functions of the plant immune hormone salicylic acid (SA) often affect plant growth. Although benzothiadiazole (BTH), a synthetic analog of SA, has been widely used to protect crops from diseases by inducing plant defense responses, we recently demonstrated that SA, but not BTH, confers resistance against Rhizoctonia solani, the causal agent of sheath blight disease, in Brachypodium distachyon. Here, we demonstrated that BTH compromised the resistance of Bd3-1 and Gaz4, the two sheath blight-resistant accessions of B. distachyon, which activate SA-dependent signaling following challenge by R. solani. Moreover, upon analyzing our published RNA-seq data from B. distachyon treated with SA or BTH, we found that BTH specifically induces expression of genes related to chloroplast function and jasmonic acid (JA) signaling, suggesting that BTH attenuates R. solani resistance by perturbing growth-defense trade-offs and/or by inducing a JA response that may increase susceptibility to R. solani. Our findings demonstrated that BTH does not work as a simple mimic of SA in B. distachyon, and consequently may presumably cause unfavorable side effects through the transcriptional alteration, particularly with respect to R. solani resistance.",
author = "Yusuke Kouzai and Yoshiteru Noutoshi and Komaki Inoue and Minami Shimizu and Yoshihiko Onda and Keiichi Mochida",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-35790-w",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Benzothiadiazole, a plant defense inducer, negatively regulates sheath blight resistance in Brachypodium distachyon

AU - Kouzai, Yusuke

AU - Noutoshi, Yoshiteru

AU - Inoue, Komaki

AU - Shimizu, Minami

AU - Onda, Yoshihiko

AU - Mochida, Keiichi

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Plant defense inducers that mimic functions of the plant immune hormone salicylic acid (SA) often affect plant growth. Although benzothiadiazole (BTH), a synthetic analog of SA, has been widely used to protect crops from diseases by inducing plant defense responses, we recently demonstrated that SA, but not BTH, confers resistance against Rhizoctonia solani, the causal agent of sheath blight disease, in Brachypodium distachyon. Here, we demonstrated that BTH compromised the resistance of Bd3-1 and Gaz4, the two sheath blight-resistant accessions of B. distachyon, which activate SA-dependent signaling following challenge by R. solani. Moreover, upon analyzing our published RNA-seq data from B. distachyon treated with SA or BTH, we found that BTH specifically induces expression of genes related to chloroplast function and jasmonic acid (JA) signaling, suggesting that BTH attenuates R. solani resistance by perturbing growth-defense trade-offs and/or by inducing a JA response that may increase susceptibility to R. solani. Our findings demonstrated that BTH does not work as a simple mimic of SA in B. distachyon, and consequently may presumably cause unfavorable side effects through the transcriptional alteration, particularly with respect to R. solani resistance.

AB - Plant defense inducers that mimic functions of the plant immune hormone salicylic acid (SA) often affect plant growth. Although benzothiadiazole (BTH), a synthetic analog of SA, has been widely used to protect crops from diseases by inducing plant defense responses, we recently demonstrated that SA, but not BTH, confers resistance against Rhizoctonia solani, the causal agent of sheath blight disease, in Brachypodium distachyon. Here, we demonstrated that BTH compromised the resistance of Bd3-1 and Gaz4, the two sheath blight-resistant accessions of B. distachyon, which activate SA-dependent signaling following challenge by R. solani. Moreover, upon analyzing our published RNA-seq data from B. distachyon treated with SA or BTH, we found that BTH specifically induces expression of genes related to chloroplast function and jasmonic acid (JA) signaling, suggesting that BTH attenuates R. solani resistance by perturbing growth-defense trade-offs and/or by inducing a JA response that may increase susceptibility to R. solani. Our findings demonstrated that BTH does not work as a simple mimic of SA in B. distachyon, and consequently may presumably cause unfavorable side effects through the transcriptional alteration, particularly with respect to R. solani resistance.

UR - http://www.scopus.com/inward/record.url?scp=85057143342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057143342&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-35790-w

DO - 10.1038/s41598-018-35790-w

M3 - Article

C2 - 30478396

AN - SCOPUS:85057143342

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 17358

ER -