Behavior of enclosed sol- and gel-alginates in vivo

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Changes in the behavior of alginates, both non-gelled and gelled with calcium ions, in microcapsules after implantation in vivo were investigated using alginates enclosed in regenerated cellulose membrane. The mechanical properties of the alginates after soaking in simulated body fluid (SBF) and implantation in the peritoneal cavities of animals were determined. A stress-compression study on specimens soaked in SBF showed that the alginates in the microcapsules would have almost the same mechanical properties after several days of implantation independent of whether the enclosed alginate was non-gelled or gelled with calcium ions before implantation. The stresses at a particular compression point decreased with increasing incubation period for 3-6 days for the alginates gelled with calcium ions before soaking in SBF. In contrast, these values increased over the same period for the non-gelled alginates before soaking in SBF. In agreement with the results of the in vitro study, no significant differences were observed between the mechanical properties of the non-gelled and gelled alginates retrieved from the peritoneal cavities of DDY mice after 1 week of implantation.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalBiochemical Engineering Journal
Volume22
Issue number1
DOIs
Publication statusPublished - Dec 2004
Externally publishedYes

Fingerprint

Alginates
Alginate
Polymethyl Methacrylate
Sols
Gels
Body fluids
Body Fluids
Calcium
Peritoneal Cavity
Ions
Mechanical properties
Capsules
Ion implantation
Cellulose
Animals
Compaction
Membranes

Keywords

  • Algae
  • Biomedical
  • Immobilized cells
  • Microcapsule
  • Simulated body fluid
  • Tissue cell culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Chemical Engineering(all)

Cite this

Behavior of enclosed sol- and gel-alginates in vivo. / Sakai, Shinji; Ono, Tsutomu; Ijima, Hiroyuki; Kawakami, Koei.

In: Biochemical Engineering Journal, Vol. 22, No. 1, 12.2004, p. 19-24.

Research output: Contribution to journalArticle

Sakai, Shinji ; Ono, Tsutomu ; Ijima, Hiroyuki ; Kawakami, Koei. / Behavior of enclosed sol- and gel-alginates in vivo. In: Biochemical Engineering Journal. 2004 ; Vol. 22, No. 1. pp. 19-24.
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