BEC, a novel enterotoxin of clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks

Shinya Yonogi, Shigeaki Matsuda, Takao Kawai, Tomoko Yoda, Tetsuya Harada, Yuko Kumeda, Kazuyoshi Goto, Hirotaka Hiyoshi, Shota Nakamura, Toshio Kodama, Tetsuya Iida

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Clostridium perfringens is a causative agent of food-borne gastroenteritis for which C. perfringens enterotoxin (CPE) has been considered an essential factor. Recently, we experienced two outbreaks of food-borne gastroenteritis in which non-CPE producers of C. perfringens were strongly suspected to be the cause. Here, we report a novel enterotoxin produced by C. perfringens isolates, BEC (binary enterotoxin of C. perfringens). Culture supernatants of the C. perfringens strains showed fluid-accumulating activity in rabbit ileal loop and suckling mouse assays. Purification of the enterotoxic substance in the supernatants and high-throughput sequencing of genomic DNA of the strains revealed BEC, composed of BECa and BECb. BECa and BECb displayed limited amino acid sequence similarity to other binary toxin family members, such as the C. perfringens iota toxin. The becAB genes were located on 54.5-kb pCP13-like plasmids. Recombinant BECb (rBECb) alone had fluid-accumulating activity in the suckling mouse assay. Although rBECa alone did not show enterotoxic activity, rBECa enhanced the enterotoxicity of rBECb when simultaneously administered in suckling mice. The entertoxicity of the mutant in which the becB gene was disrupted was dramatically decreased compared to that of the parental strain. rBECa showed an ADP-ribosylating activity on purified actin. Although we have not directly evaluated whether BECb delivers BECa into cells, rounding of Vero cells occurred only when cells were treated with both rBECa and rBECb. These results suggest that BEC is a novel enterotoxin of C. perfringens distinct from CPE, and that BEC-producing C. perfringens strains can be causative agents of acute gastroenteritis in humans. Additionally, the presence of becAB on nearly identical plasmids in distinct lineages of C. perfringens isolates suggests the involvement of horizontal gene transfer in the acquisition of the toxin genes.

Original languageEnglish
Pages (from-to)2390-2399
Number of pages10
JournalInfection and Immunity
Volume82
Issue number6
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Gastroenteritis
Disease Outbreaks
Clostridium perfringens
Plasmids
Clostridium enterotoxin
Genes
High-Throughput Nucleotide Sequencing
Food
Horizontal Gene Transfer
Vero Cells
Adenosine Diphosphate
Actins
Amino Acid Sequence
Rabbits

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases
  • Medicine(all)

Cite this

BEC, a novel enterotoxin of clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks. / Yonogi, Shinya; Matsuda, Shigeaki; Kawai, Takao; Yoda, Tomoko; Harada, Tetsuya; Kumeda, Yuko; Goto, Kazuyoshi; Hiyoshi, Hirotaka; Nakamura, Shota; Kodama, Toshio; Iida, Tetsuya.

In: Infection and Immunity, Vol. 82, No. 6, 2014, p. 2390-2399.

Research output: Contribution to journalArticle

Yonogi, S, Matsuda, S, Kawai, T, Yoda, T, Harada, T, Kumeda, Y, Goto, K, Hiyoshi, H, Nakamura, S, Kodama, T & Iida, T 2014, 'BEC, a novel enterotoxin of clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks', Infection and Immunity, vol. 82, no. 6, pp. 2390-2399. https://doi.org/10.1128/IAI.01759-14
Yonogi, Shinya ; Matsuda, Shigeaki ; Kawai, Takao ; Yoda, Tomoko ; Harada, Tetsuya ; Kumeda, Yuko ; Goto, Kazuyoshi ; Hiyoshi, Hirotaka ; Nakamura, Shota ; Kodama, Toshio ; Iida, Tetsuya. / BEC, a novel enterotoxin of clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks. In: Infection and Immunity. 2014 ; Vol. 82, No. 6. pp. 2390-2399.
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abstract = "Clostridium perfringens is a causative agent of food-borne gastroenteritis for which C. perfringens enterotoxin (CPE) has been considered an essential factor. Recently, we experienced two outbreaks of food-borne gastroenteritis in which non-CPE producers of C. perfringens were strongly suspected to be the cause. Here, we report a novel enterotoxin produced by C. perfringens isolates, BEC (binary enterotoxin of C. perfringens). Culture supernatants of the C. perfringens strains showed fluid-accumulating activity in rabbit ileal loop and suckling mouse assays. Purification of the enterotoxic substance in the supernatants and high-throughput sequencing of genomic DNA of the strains revealed BEC, composed of BECa and BECb. BECa and BECb displayed limited amino acid sequence similarity to other binary toxin family members, such as the C. perfringens iota toxin. The becAB genes were located on 54.5-kb pCP13-like plasmids. Recombinant BECb (rBECb) alone had fluid-accumulating activity in the suckling mouse assay. Although rBECa alone did not show enterotoxic activity, rBECa enhanced the enterotoxicity of rBECb when simultaneously administered in suckling mice. The entertoxicity of the mutant in which the becB gene was disrupted was dramatically decreased compared to that of the parental strain. rBECa showed an ADP-ribosylating activity on purified actin. Although we have not directly evaluated whether BECb delivers BECa into cells, rounding of Vero cells occurred only when cells were treated with both rBECa and rBECb. These results suggest that BEC is a novel enterotoxin of C. perfringens distinct from CPE, and that BEC-producing C. perfringens strains can be causative agents of acute gastroenteritis in humans. Additionally, the presence of becAB on nearly identical plasmids in distinct lineages of C. perfringens isolates suggests the involvement of horizontal gene transfer in the acquisition of the toxin genes.",
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